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Presence of antibiotic-resistant commensal bacteria in samples from agricultural, city, and national park environments evaluated by standard culture and real-time PCR methods.
Can J Microbiol. 2010 Sep; 56(9):761-70.CJ

Abstract

This study examined the presence of antibiotic-resistant commensal bacteria among cattle operations representing areas heavily affected by agriculture, city locations representing areas affected by urban activities and indirectly affected by agriculture, and a national park representing an area not affected by agriculture. A total of 288 soil, fecal floor, and water samples were collected from cattle operations, from the city of Fort Collins, and from Rocky Mountain National Park (RMNP) in Colorado. In addition, a total of 42 new and unused feed, unused bedding, compost, and manure samples were obtained from the cattle operations. Total, tetracycline-resistant, and ceftiofur-resistant bacterial populations were enumerated by both standard culture plating and real-time PCR methods. Only wastewater samples from the cattle operations demonstrated both higher tetracycline-resistant bacterial counts (enumerated by the culture plating method) and tetracycline resistance gene copies (quantified by real-time PCR) compared to water samples collected from non-farm environments. The ceftiofur resistance gene, blaCMY-2, was not detectable in any of the samples, while the tetracycline resistance genes examined in this study, tet(B), tet(C), tet(W), and tet(O), were detected in all types of tested samples, except soil samples from RMNP. Tetracycline resistance gene pools quantified from the tet(O) and tet(W) genes were bigger than those from the tet(B) and tet(C) genes in fecal and water samples. Although only limited resistance genes, instead of a full set, were selected for real-time PCR quantification in this study, our results point to the need for further studies to determine natural and urban impacts on antibiotic resistance.

Authors+Show Affiliations

Center for Meat Safety and Quality, Food Safety Cluster, Department of Animal Sciences, Colorado State University, 1171 Campus Delivery, Fort Collins, CO 80523-1171, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20921986

Citation

Yang, Hua, et al. "Presence of Antibiotic-resistant Commensal Bacteria in Samples From Agricultural, City, and National Park Environments Evaluated By Standard Culture and Real-time PCR Methods." Canadian Journal of Microbiology, vol. 56, no. 9, 2010, pp. 761-70.
Yang H, Byelashov OA, Geornaras I, et al. Presence of antibiotic-resistant commensal bacteria in samples from agricultural, city, and national park environments evaluated by standard culture and real-time PCR methods. Can J Microbiol. 2010;56(9):761-70.
Yang, H., Byelashov, O. A., Geornaras, I., Goodridge, L. D., Nightingale, K. K., Belk, K. E., Smith, G. C., & Sofos, J. N. (2010). Presence of antibiotic-resistant commensal bacteria in samples from agricultural, city, and national park environments evaluated by standard culture and real-time PCR methods. Canadian Journal of Microbiology, 56(9), 761-70. https://doi.org/10.1139/w10-060
Yang H, et al. Presence of Antibiotic-resistant Commensal Bacteria in Samples From Agricultural, City, and National Park Environments Evaluated By Standard Culture and Real-time PCR Methods. Can J Microbiol. 2010;56(9):761-70. PubMed PMID: 20921986.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Presence of antibiotic-resistant commensal bacteria in samples from agricultural, city, and national park environments evaluated by standard culture and real-time PCR methods. AU - Yang,Hua, AU - Byelashov,Oleksandr A, AU - Geornaras,Ifigenia, AU - Goodridge,Lawrence D, AU - Nightingale,Kendra K, AU - Belk,Keith E, AU - Smith,Gary C, AU - Sofos,John N, PY - 2010/10/6/entrez PY - 2010/10/6/pubmed PY - 2010/12/14/medline SP - 761 EP - 70 JF - Canadian journal of microbiology JO - Can J Microbiol VL - 56 IS - 9 N2 - This study examined the presence of antibiotic-resistant commensal bacteria among cattle operations representing areas heavily affected by agriculture, city locations representing areas affected by urban activities and indirectly affected by agriculture, and a national park representing an area not affected by agriculture. A total of 288 soil, fecal floor, and water samples were collected from cattle operations, from the city of Fort Collins, and from Rocky Mountain National Park (RMNP) in Colorado. In addition, a total of 42 new and unused feed, unused bedding, compost, and manure samples were obtained from the cattle operations. Total, tetracycline-resistant, and ceftiofur-resistant bacterial populations were enumerated by both standard culture plating and real-time PCR methods. Only wastewater samples from the cattle operations demonstrated both higher tetracycline-resistant bacterial counts (enumerated by the culture plating method) and tetracycline resistance gene copies (quantified by real-time PCR) compared to water samples collected from non-farm environments. The ceftiofur resistance gene, blaCMY-2, was not detectable in any of the samples, while the tetracycline resistance genes examined in this study, tet(B), tet(C), tet(W), and tet(O), were detected in all types of tested samples, except soil samples from RMNP. Tetracycline resistance gene pools quantified from the tet(O) and tet(W) genes were bigger than those from the tet(B) and tet(C) genes in fecal and water samples. Although only limited resistance genes, instead of a full set, were selected for real-time PCR quantification in this study, our results point to the need for further studies to determine natural and urban impacts on antibiotic resistance. SN - 1480-3275 UR - https://www.unboundmedicine.com/medline/citation/20921986/Presence_of_antibiotic_resistant_commensal_bacteria_in_samples_from_agricultural_city_and_national_park_environments_evaluated_by_standard_culture_and_real_time_PCR_methods_ L2 - https://cdnsciencepub.com/doi/10.1139/w10-060?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -