Tags

Type your tag names separated by a space and hit enter

Proinflammatory effect of cholesterol and its oxidation products on CaCo-2 human enterocyte-like cells: effective protection by epigallocatechin-3-gallate.
Free Radic Biol Med. 2010 Dec 15; 49(12):2049-57.FR

Abstract

Cholesterol and its oxidation products, namely oxysterols, have very recently been shown to potentially interfere with homeostasis of the human digestive tract, by promoting and sustaining irreversible damage of the colonic epithelial layer. This report concerns the strong proinflammatory action that a dietary oxysterol mixture and, to a lesser extent, an identical concentration of unoxidized cholesterol exert on CaCo-2 colonic epithelial cells by up-regulating both expression and synthesis of interleukin 8. The oxysterol mixture and its most effective component, 7β-hydroxycholesterol, are also shown to markedly enhance the expression of key inflammatory and chemotactic cytokines in colonic epithelial cells, more efficiently than unoxidized cholesterol. The sterols' proinflammatory effect seems to be mediated by enhanced activation of NOX1, because it is prevented by pretreatment of the cells with DPI, a selective inhibitor of this oxidase. Importantly, NOX1 hyperactivation by the oxysterol mixture or cholesterol was fully prevented by CaCo-2 cell preincubation with epigallocatechin-3-gallate. Consistently, supplementation with this compound fully protected colonic epithelial cells against overexpression of inflammatory and chemotactic genes induced by the sterols investigated.

Authors+Show Affiliations

Department of Clinical and Biological Sciences, University of Turin at San Luigi Gonzaga Hospital, Turin, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20923702

Citation

Mascia, Cinzia, et al. "Proinflammatory Effect of Cholesterol and Its Oxidation Products On CaCo-2 Human Enterocyte-like Cells: Effective Protection By Epigallocatechin-3-gallate." Free Radical Biology & Medicine, vol. 49, no. 12, 2010, pp. 2049-57.
Mascia C, Maina M, Chiarpotto E, et al. Proinflammatory effect of cholesterol and its oxidation products on CaCo-2 human enterocyte-like cells: effective protection by epigallocatechin-3-gallate. Free Radic Biol Med. 2010;49(12):2049-57.
Mascia, C., Maina, M., Chiarpotto, E., Leonarduzzi, G., Poli, G., & Biasi, F. (2010). Proinflammatory effect of cholesterol and its oxidation products on CaCo-2 human enterocyte-like cells: effective protection by epigallocatechin-3-gallate. Free Radical Biology & Medicine, 49(12), 2049-57. https://doi.org/10.1016/j.freeradbiomed.2010.09.033
Mascia C, et al. Proinflammatory Effect of Cholesterol and Its Oxidation Products On CaCo-2 Human Enterocyte-like Cells: Effective Protection By Epigallocatechin-3-gallate. Free Radic Biol Med. 2010 Dec 15;49(12):2049-57. PubMed PMID: 20923702.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Proinflammatory effect of cholesterol and its oxidation products on CaCo-2 human enterocyte-like cells: effective protection by epigallocatechin-3-gallate. AU - Mascia,Cinzia, AU - Maina,Marco, AU - Chiarpotto,Elena, AU - Leonarduzzi,Gabriella, AU - Poli,Giuseppe, AU - Biasi,Fiorella, Y1 - 2010/10/27/ PY - 2010/08/12/received PY - 2010/09/24/revised PY - 2010/09/28/accepted PY - 2010/10/7/entrez PY - 2010/10/7/pubmed PY - 2011/8/19/medline SP - 2049 EP - 57 JF - Free radical biology & medicine JO - Free Radic Biol Med VL - 49 IS - 12 N2 - Cholesterol and its oxidation products, namely oxysterols, have very recently been shown to potentially interfere with homeostasis of the human digestive tract, by promoting and sustaining irreversible damage of the colonic epithelial layer. This report concerns the strong proinflammatory action that a dietary oxysterol mixture and, to a lesser extent, an identical concentration of unoxidized cholesterol exert on CaCo-2 colonic epithelial cells by up-regulating both expression and synthesis of interleukin 8. The oxysterol mixture and its most effective component, 7β-hydroxycholesterol, are also shown to markedly enhance the expression of key inflammatory and chemotactic cytokines in colonic epithelial cells, more efficiently than unoxidized cholesterol. The sterols' proinflammatory effect seems to be mediated by enhanced activation of NOX1, because it is prevented by pretreatment of the cells with DPI, a selective inhibitor of this oxidase. Importantly, NOX1 hyperactivation by the oxysterol mixture or cholesterol was fully prevented by CaCo-2 cell preincubation with epigallocatechin-3-gallate. Consistently, supplementation with this compound fully protected colonic epithelial cells against overexpression of inflammatory and chemotactic genes induced by the sterols investigated. SN - 1873-4596 UR - https://www.unboundmedicine.com/medline/citation/20923702/Proinflammatory_effect_of_cholesterol_and_its_oxidation_products_on_CaCo_2_human_enterocyte_like_cells:_effective_protection_by_epigallocatechin_3_gallate_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0891-5849(10)00598-8 DB - PRIME DP - Unbound Medicine ER -