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Regulation of lung cancer cell migration and invasion by reactive oxygen species and caveolin-1.
J Biol Chem. 2010 Dec 10; 285(50):38832-40.JB

Abstract

The acquired capability of tumor cells to migrate and invade neighboring tissues is associated with high metastatic potential and advanced stage of cancers. Recently, signaling molecules such as reactive oxygen species (ROS) and caveolin-1 (Cav-1) have been implicated in the aggressive behavior of cancer cells. However, the roles of specific ROS in cancer cell migration and Cav-1 regulation are unclear. We demonstrate here that Cav-1 plays an important role in the migration and invasion of human lung carcinoma H460 cells and that these effects are differentially regulated by cellular ROS. Using various known inhibitors and donors of ROS, we found that different ROS have different effects on Cav-1 expression and cell migration and invasion. Superoxide anion and hydrogen peroxide down-regulated Cav-1 expression and inhibited cell migration and invasion, whereas hydroxyl radical up-regulated the Cav-1 expression and promoted cell migration and invasion. The down-regulating effect of superoxide anion and hydrogen peroxide on Cav-1 is mediated through a transcription-independent mechanism that involves protein degradation via the ubiquitin-proteasome pathway. These results indicate the essential role of different ROS in cancer cell motility and through Cav-1 expression, which may provide a key mechanism controlling tumor progression and metastasis. The up-regulation of Cav-1 and cell motility by hydroxyl free radical suggests an important role of this ROS as a positive regulator of tumor progression.

Authors+Show Affiliations

Pharmaceutical Technology (International) Program, Chulalongkorn University, Bangkok 10330, Thailand.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20923773

Citation

Luanpitpong, Sudjit, et al. "Regulation of Lung Cancer Cell Migration and Invasion By Reactive Oxygen Species and Caveolin-1." The Journal of Biological Chemistry, vol. 285, no. 50, 2010, pp. 38832-40.
Luanpitpong S, Talbott SJ, Rojanasakul Y, et al. Regulation of lung cancer cell migration and invasion by reactive oxygen species and caveolin-1. J Biol Chem. 2010;285(50):38832-40.
Luanpitpong, S., Talbott, S. J., Rojanasakul, Y., Nimmannit, U., Pongrakhananon, V., Wang, L., & Chanvorachote, P. (2010). Regulation of lung cancer cell migration and invasion by reactive oxygen species and caveolin-1. The Journal of Biological Chemistry, 285(50), 38832-40. https://doi.org/10.1074/jbc.M110.124958
Luanpitpong S, et al. Regulation of Lung Cancer Cell Migration and Invasion By Reactive Oxygen Species and Caveolin-1. J Biol Chem. 2010 Dec 10;285(50):38832-40. PubMed PMID: 20923773.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Regulation of lung cancer cell migration and invasion by reactive oxygen species and caveolin-1. AU - Luanpitpong,Sudjit, AU - Talbott,Siera Jo, AU - Rojanasakul,Yon, AU - Nimmannit,Ubonthip, AU - Pongrakhananon,Varisa, AU - Wang,Liying, AU - Chanvorachote,Pithi, Y1 - 2010/10/05/ PY - 2010/10/7/entrez PY - 2010/10/7/pubmed PY - 2011/1/11/medline SP - 38832 EP - 40 JF - The Journal of biological chemistry JO - J. Biol. Chem. VL - 285 IS - 50 N2 - The acquired capability of tumor cells to migrate and invade neighboring tissues is associated with high metastatic potential and advanced stage of cancers. Recently, signaling molecules such as reactive oxygen species (ROS) and caveolin-1 (Cav-1) have been implicated in the aggressive behavior of cancer cells. However, the roles of specific ROS in cancer cell migration and Cav-1 regulation are unclear. We demonstrate here that Cav-1 plays an important role in the migration and invasion of human lung carcinoma H460 cells and that these effects are differentially regulated by cellular ROS. Using various known inhibitors and donors of ROS, we found that different ROS have different effects on Cav-1 expression and cell migration and invasion. Superoxide anion and hydrogen peroxide down-regulated Cav-1 expression and inhibited cell migration and invasion, whereas hydroxyl radical up-regulated the Cav-1 expression and promoted cell migration and invasion. The down-regulating effect of superoxide anion and hydrogen peroxide on Cav-1 is mediated through a transcription-independent mechanism that involves protein degradation via the ubiquitin-proteasome pathway. These results indicate the essential role of different ROS in cancer cell motility and through Cav-1 expression, which may provide a key mechanism controlling tumor progression and metastasis. The up-regulation of Cav-1 and cell motility by hydroxyl free radical suggests an important role of this ROS as a positive regulator of tumor progression. SN - 1083-351X UR - https://www.unboundmedicine.com/medline/citation/20923773/Regulation_of_lung_cancer_cell_migration_and_invasion_by_reactive_oxygen_species_and_caveolin_1_ L2 - http://www.jbc.org/cgi/pmidlookup?view=long&pmid=20923773 DB - PRIME DP - Unbound Medicine ER -