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Sugar-hormone cross-talk in seed development: two redundant pathways of IAA biosynthesis are regulated differentially in the invertase-deficient miniature1 (mn1) seed mutant in maize.
Mol Plant. 2010 Nov; 3(6):1026-36.MP

Abstract

The miniature1 (mn1) seed phenotype is a loss-of-function mutation at the Mn1 locus that encodes a cell wall invertase; its deficiency leads to pleiotropic changes including altered sugar levels and decreased levels of IAA throughout seed development. To understand the molecular details of such a sugar-hormone relationship, we have initiated studies on IAA biosynthesis genes in developing seeds of maize. Two tryptophan-dependent pathways of IAA biosynthesis, tryptamine (TAM) and indole-3-pyruvic acid (IPA), are of particular interest. We report on molecular isolation and characterization of an endosperm-specific ZmTARelated1 (ZmTar1) gene of the IPA branch; we have also reported recently on ZmYuc1 gene in the TAM branch. Comparative gene expression analyses here have shown that (1) the ZmTar1 transcripts were approximately 10-fold higher levels than the ZmYuc1; (2) although both genes showed the highest level of expression at 8-12 d after pollination (DAP) coincident with an early peak in IAA levels, the two showed highly divergent (antagonistic) response at 12 and 16 DAP but similar patterns at 20 and 28 DAP in the Mn1 and mn1 endosperm. The Western blot analyses for the ZmTAR1 protein, however, displayed disconcordant protein/transcript expression patterns. Overall, these data report novel observations on redundant trp-dependent pathways of auxin biosynthesis in developing seeds of maize, and suggest that homeostatic control of IAA in this important sink is highly complex and may be regulated by both sucrose metabolism and developmental signals.

Authors+Show Affiliations

USDA-ARS, Center for Medical, Agricultural and Veterinary Entomology, Gainesville, FL 32608-1069, USA. Prem.Chourey@ars.usda.govNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20924026

Citation

Chourey, Prem S., et al. "Sugar-hormone Cross-talk in Seed Development: Two Redundant Pathways of IAA Biosynthesis Are Regulated Differentially in the Invertase-deficient Miniature1 (mn1) Seed Mutant in Maize." Molecular Plant, vol. 3, no. 6, 2010, pp. 1026-36.
Chourey PS, Li QB, Kumar D. Sugar-hormone cross-talk in seed development: two redundant pathways of IAA biosynthesis are regulated differentially in the invertase-deficient miniature1 (mn1) seed mutant in maize. Mol Plant. 2010;3(6):1026-36.
Chourey, P. S., Li, Q. B., & Kumar, D. (2010). Sugar-hormone cross-talk in seed development: two redundant pathways of IAA biosynthesis are regulated differentially in the invertase-deficient miniature1 (mn1) seed mutant in maize. Molecular Plant, 3(6), 1026-36. https://doi.org/10.1093/mp/ssq057
Chourey PS, Li QB, Kumar D. Sugar-hormone Cross-talk in Seed Development: Two Redundant Pathways of IAA Biosynthesis Are Regulated Differentially in the Invertase-deficient Miniature1 (mn1) Seed Mutant in Maize. Mol Plant. 2010;3(6):1026-36. PubMed PMID: 20924026.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sugar-hormone cross-talk in seed development: two redundant pathways of IAA biosynthesis are regulated differentially in the invertase-deficient miniature1 (mn1) seed mutant in maize. AU - Chourey,Prem S, AU - Li,Qin-Bao, AU - Kumar,Dibyendu, Y1 - 2010/10/05/ PY - 2010/10/7/entrez PY - 2010/10/7/pubmed PY - 2011/4/20/medline SP - 1026 EP - 36 JF - Molecular plant JO - Mol Plant VL - 3 IS - 6 N2 - The miniature1 (mn1) seed phenotype is a loss-of-function mutation at the Mn1 locus that encodes a cell wall invertase; its deficiency leads to pleiotropic changes including altered sugar levels and decreased levels of IAA throughout seed development. To understand the molecular details of such a sugar-hormone relationship, we have initiated studies on IAA biosynthesis genes in developing seeds of maize. Two tryptophan-dependent pathways of IAA biosynthesis, tryptamine (TAM) and indole-3-pyruvic acid (IPA), are of particular interest. We report on molecular isolation and characterization of an endosperm-specific ZmTARelated1 (ZmTar1) gene of the IPA branch; we have also reported recently on ZmYuc1 gene in the TAM branch. Comparative gene expression analyses here have shown that (1) the ZmTar1 transcripts were approximately 10-fold higher levels than the ZmYuc1; (2) although both genes showed the highest level of expression at 8-12 d after pollination (DAP) coincident with an early peak in IAA levels, the two showed highly divergent (antagonistic) response at 12 and 16 DAP but similar patterns at 20 and 28 DAP in the Mn1 and mn1 endosperm. The Western blot analyses for the ZmTAR1 protein, however, displayed disconcordant protein/transcript expression patterns. Overall, these data report novel observations on redundant trp-dependent pathways of auxin biosynthesis in developing seeds of maize, and suggest that homeostatic control of IAA in this important sink is highly complex and may be regulated by both sucrose metabolism and developmental signals. SN - 1752-9867 UR - https://www.unboundmedicine.com/medline/citation/20924026/Sugar_hormone_cross_talk_in_seed_development:_two_redundant_pathways_of_IAA_biosynthesis_are_regulated_differentially_in_the_invertase_deficient_miniature1__mn1__seed_mutant_in_maize_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1674-2052(14)60551-7 DB - PRIME DP - Unbound Medicine ER -