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[Pernicious anaemia--diagnostic benefit of the detection of autoantibodies against intrinsic factor and gastric parietal cells antigen H+/K+ ATPase].
Epidemiol Mikrobiol Imunol. 2010 Aug; 59(3):126-32.EM

Abstract

BACKGROUND

Pernicious anaemia is an autoimmune disease that causes acquired vitamin B12 deficiency. The diagnostic process includes the detection of typical changes in the blood count, low serum levels of vitamin B12, endoscopic and histological signs of gastritis and autoantibodies against the gastric parietal cells antigen H+/K+ ATPase and intrinsic factor.

OBJECTIVE

Our aims were to establish immunological tests for the detection of autoantibodies against intrinsic factor and target gastric parietal cell antigen H+/K+ ATPase and to evaluate their diagnostic benefits in patients with pernicious anaemia.

MATERIAL AND METHODS

Sera from 95 patients were tested for autoantibodies against H+/K+ ATPase and intrinsic factor by multiplex Luminex assay. The results were compared with those of the immunofluorescence assay for the detection of autoantibodies against gastric parietal cells and with the diagnostic criteria.

RESULTS

The autoantibodies against gastric parietal cell H+/K+ ATPase had a sensitivity of 68.2% with a specificity of 91.7% for the diagnosis of pernicious anaemia. The respective rates for the autoantibodies against intrinsic factor were 40.9% and 98.6%. The combined sensitivity and specificity rates for both autoantibodies were 86.36% and 90.28%, respectively, the combined positive predictive value was 73.08% and the combined negative predictive value was 95.59%.

CONCLUSION

The detection of both autoantibodies is helpful in diagnosing pernicious anaemia and the combination of the two assays increases diagnostic sensitivity.

Authors+Show Affiliations

Oddelení klinické biochemie, hematologie a imunologie, Nemocnice Na Homolce, Praha. lenka.sedlackova@homolka.czNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

cze

PubMed ID

20925249

Citation

Sedláková, L, et al. "[Pernicious Anaemia--diagnostic Benefit of the Detection of Autoantibodies Against Intrinsic Factor and Gastric Parietal Cells Antigen H+/K+ ATPase]." Epidemiologie, Mikrobiologie, Imunologie : Casopis Spolecnosti Pro Epidemiologii a Mikrobiologii Ceske Lekarske Spolecnosti J.E. Purkyne, vol. 59, no. 3, 2010, pp. 126-32.
Sedláková L, Dubská L, Průcha M. [Pernicious anaemia--diagnostic benefit of the detection of autoantibodies against intrinsic factor and gastric parietal cells antigen H+/K+ ATPase]. Epidemiol Mikrobiol Imunol. 2010;59(3):126-32.
Sedláková, L., Dubská, L., & Průcha, M. (2010). [Pernicious anaemia--diagnostic benefit of the detection of autoantibodies against intrinsic factor and gastric parietal cells antigen H+/K+ ATPase]. Epidemiologie, Mikrobiologie, Imunologie : Casopis Spolecnosti Pro Epidemiologii a Mikrobiologii Ceske Lekarske Spolecnosti J.E. Purkyne, 59(3), 126-32.
Sedláková L, Dubská L, Průcha M. [Pernicious Anaemia--diagnostic Benefit of the Detection of Autoantibodies Against Intrinsic Factor and Gastric Parietal Cells Antigen H+/K+ ATPase]. Epidemiol Mikrobiol Imunol. 2010;59(3):126-32. PubMed PMID: 20925249.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Pernicious anaemia--diagnostic benefit of the detection of autoantibodies against intrinsic factor and gastric parietal cells antigen H+/K+ ATPase]. AU - Sedláková,L, AU - Dubská,L, AU - Průcha,M, PY - 2010/10/8/entrez PY - 2010/10/12/pubmed PY - 2010/10/29/medline SP - 126 EP - 32 JF - Epidemiologie, mikrobiologie, imunologie : casopis Spolecnosti pro epidemiologii a mikrobiologii Ceske lekarske spolecnosti J.E. Purkyne JO - Epidemiol Mikrobiol Imunol VL - 59 IS - 3 N2 - BACKGROUND: Pernicious anaemia is an autoimmune disease that causes acquired vitamin B12 deficiency. The diagnostic process includes the detection of typical changes in the blood count, low serum levels of vitamin B12, endoscopic and histological signs of gastritis and autoantibodies against the gastric parietal cells antigen H+/K+ ATPase and intrinsic factor. OBJECTIVE: Our aims were to establish immunological tests for the detection of autoantibodies against intrinsic factor and target gastric parietal cell antigen H+/K+ ATPase and to evaluate their diagnostic benefits in patients with pernicious anaemia. MATERIAL AND METHODS: Sera from 95 patients were tested for autoantibodies against H+/K+ ATPase and intrinsic factor by multiplex Luminex assay. The results were compared with those of the immunofluorescence assay for the detection of autoantibodies against gastric parietal cells and with the diagnostic criteria. RESULTS: The autoantibodies against gastric parietal cell H+/K+ ATPase had a sensitivity of 68.2% with a specificity of 91.7% for the diagnosis of pernicious anaemia. The respective rates for the autoantibodies against intrinsic factor were 40.9% and 98.6%. The combined sensitivity and specificity rates for both autoantibodies were 86.36% and 90.28%, respectively, the combined positive predictive value was 73.08% and the combined negative predictive value was 95.59%. CONCLUSION: The detection of both autoantibodies is helpful in diagnosing pernicious anaemia and the combination of the two assays increases diagnostic sensitivity. SN - 1210-7913 UR - https://www.unboundmedicine.com/medline/citation/20925249/[Pernicious_anaemia__diagnostic_benefit_of_the_detection_of_autoantibodies_against_intrinsic_factor_and_gastric_parietal_cells_antigen_H+/K+_ATPase]_ DB - PRIME DP - Unbound Medicine ER -