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Molecular characterization and antimicrobial susceptibility of extended-spectrum {beta}-lactamase-producing Enterobacteriaceae isolates at a tertiary-care centre in Monterrey, Mexico.
J Med Microbiol. 2011 Jan; 60(Pt 1):84-90.JM

Abstract

Our objective was to analyse phenotypic and genetic data of extended-spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae, Enterobacter cloacae, Escherichia coli and Serratia marcescens that cause infections in our hospital. Over a 3 year period, 342 randomly selected clinical Enterobacteriaceae isolates were tested for ESBL production and evaluated for the presence of the β-lactamase genes bla(SHV), bla(TEM,) bla(CTX-M) and bla(TLA-1). The antibiotic susceptibilities of these isolates were also determined, and the clonality of the isolates was assessed by PFGE. Based on our analyses, 33/92 (35.9 %) K. pneumoniae, 31/87 (35.6 %) Enterobacter cloacae, 24/80 (30 %) E. coli and 17/83 (20.5 %) S. marcescens were identified as ESBL producers. The presence of TEM, SHV or CTX ESBL types was detected in 99/105 (94 %) of the isolates. TLA-1 was not detected in any of the 105 isolates. The dominant ESBL types were bla(SHV-5) (n=33), bla(SHV12) (n=31) and bla(CTX-M-15) (n=30). The predominant ESBL identified in E. coli and Enterobacter cloacae isolates was CTX-M-15, whereas in K. pneumoniae and S. marcescens the predominant types were SHV-12 and SHV-5, respectively. PFGE genotyping revealed two main genetic patterns in the K. pneumoniae isolates, types SHV-12 and TEM-1+SHV-5. An outbreak caused by Enterobacter cloacae SHV-5+CTX-M-15 was detected. In contrast, most ESBL-producing isolates of E. coli and S. marcescens did not have similar PFGE banding patterns and thus were not genetically similar. Enterobacteriaceae are a concern in our hospital, especially K. pneumoniae and Enterobacter cloacae. Our results confirm that the CTX-M-15 ESBL type has spread rapidly in the hospital, and thus requires careful monitoring.

Authors+Show Affiliations

Departamento de Microbiología, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, Mexico. elvira_garza_gzz@yahoo.comNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20930052

Citation

Garza-González, Elvira, et al. "Molecular Characterization and Antimicrobial Susceptibility of Extended-spectrum {beta}-lactamase-producing Enterobacteriaceae Isolates at a Tertiary-care Centre in Monterrey, Mexico." Journal of Medical Microbiology, vol. 60, no. Pt 1, 2011, pp. 84-90.
Garza-González E, Mendoza Ibarra SI, Llaca-Díaz JM, et al. Molecular characterization and antimicrobial susceptibility of extended-spectrum {beta}-lactamase-producing Enterobacteriaceae isolates at a tertiary-care centre in Monterrey, Mexico. J Med Microbiol. 2011;60(Pt 1):84-90.
Garza-González, E., Mendoza Ibarra, S. I., Llaca-Díaz, J. M., & Gonzalez, G. M. (2011). Molecular characterization and antimicrobial susceptibility of extended-spectrum {beta}-lactamase-producing Enterobacteriaceae isolates at a tertiary-care centre in Monterrey, Mexico. Journal of Medical Microbiology, 60(Pt 1), 84-90. https://doi.org/10.1099/jmm.0.022970-0
Garza-González E, et al. Molecular Characterization and Antimicrobial Susceptibility of Extended-spectrum {beta}-lactamase-producing Enterobacteriaceae Isolates at a Tertiary-care Centre in Monterrey, Mexico. J Med Microbiol. 2011;60(Pt 1):84-90. PubMed PMID: 20930052.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular characterization and antimicrobial susceptibility of extended-spectrum {beta}-lactamase-producing Enterobacteriaceae isolates at a tertiary-care centre in Monterrey, Mexico. AU - Garza-González,Elvira, AU - Mendoza Ibarra,Sandra Iveth, AU - Llaca-Díaz,Jorge M, AU - Gonzalez,Gloria M, Y1 - 2010/10/07/ PY - 2010/10/9/entrez PY - 2010/10/12/pubmed PY - 2011/1/12/medline SP - 84 EP - 90 JF - Journal of medical microbiology JO - J. Med. Microbiol. VL - 60 IS - Pt 1 N2 - Our objective was to analyse phenotypic and genetic data of extended-spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae, Enterobacter cloacae, Escherichia coli and Serratia marcescens that cause infections in our hospital. Over a 3 year period, 342 randomly selected clinical Enterobacteriaceae isolates were tested for ESBL production and evaluated for the presence of the β-lactamase genes bla(SHV), bla(TEM,) bla(CTX-M) and bla(TLA-1). The antibiotic susceptibilities of these isolates were also determined, and the clonality of the isolates was assessed by PFGE. Based on our analyses, 33/92 (35.9 %) K. pneumoniae, 31/87 (35.6 %) Enterobacter cloacae, 24/80 (30 %) E. coli and 17/83 (20.5 %) S. marcescens were identified as ESBL producers. The presence of TEM, SHV or CTX ESBL types was detected in 99/105 (94 %) of the isolates. TLA-1 was not detected in any of the 105 isolates. The dominant ESBL types were bla(SHV-5) (n=33), bla(SHV12) (n=31) and bla(CTX-M-15) (n=30). The predominant ESBL identified in E. coli and Enterobacter cloacae isolates was CTX-M-15, whereas in K. pneumoniae and S. marcescens the predominant types were SHV-12 and SHV-5, respectively. PFGE genotyping revealed two main genetic patterns in the K. pneumoniae isolates, types SHV-12 and TEM-1+SHV-5. An outbreak caused by Enterobacter cloacae SHV-5+CTX-M-15 was detected. In contrast, most ESBL-producing isolates of E. coli and S. marcescens did not have similar PFGE banding patterns and thus were not genetically similar. Enterobacteriaceae are a concern in our hospital, especially K. pneumoniae and Enterobacter cloacae. Our results confirm that the CTX-M-15 ESBL type has spread rapidly in the hospital, and thus requires careful monitoring. SN - 1473-5644 UR - https://www.unboundmedicine.com/medline/citation/20930052/Molecular_characterization_and_antimicrobial_susceptibility_of_extended_spectrum_{beta}_lactamase_producing_Enterobacteriaceae_isolates_at_a_tertiary_care_centre_in_Monterrey_Mexico_ L2 - http://jmm.microbiologyresearch.org/pubmed/content/journal/jmm/10.1099/jmm.0.022970-0 DB - PRIME DP - Unbound Medicine ER -