Tags

Type your tag names separated by a space and hit enter

Distribution of myogenic progenitor cells and myonuclei is altered in women with vs. those without chronically painful trapezius muscle.
J Appl Physiol (1985). 2010 Dec; 109(6):1920-9.JA

Abstract

It is hypothesized that repeated recruitment of low-threshold motor units is an underlying cause of chronic pain in trapezius myalgia. This study investigated the distribution of satellite cells (SCs), myonuclei, and macrophages in muscle biopsies from the trapezius muscle of 42 women performing repetitive manual work, diagnosed with trapezius myalgia (MYA; 44 ± 8 yr; mean ± SD) and 20 matched healthy controls (CON; 45 ± 9 yr). Our hypothesis was that muscle of MYA, in particular type I fibers, would demonstrate higher numbers of SCs, myonuclei, and macrophages compared with CON. SCs were identified on muscle cross sections by combined immunohistochemical staining for Pax7, type I myosin, and laminin, allowing the number of SCs associated with type I and II fibers to be determined. We observed a pattern of SC distribution in MYA previously only reported for individuals above 70 yr of age. Compared with CON, MYA demonstrated 19% more SCs per fiber associated with type I fibers (MYA 0.098 ± 0.039 vs. CON 0.079 ± 0.031; P < 0.05) and 40% fewer SCs associated with type II fibers (MYA 0.047 ± 0.017 vs. CON 0.066 ± 0.035; P < 0.05). The finding of similar numbers of macrophages between the two groups was not in line with our hypothesis and suggests that the elevated SC content of MYA was not due to heightened inflammatory cell contents, but rather to provide new myonuclei. The findings of greater numbers of SCs in type I fibers of muscle subjected to repeated low-intensity work support our hypothesis and provide new insight into stimuli capable of regulating SC content.

Authors+Show Affiliations

Institute of Sports Medicine Copenhagen, Bispebjerg Hospital, Bldg. 8, Bispebjerg Bakke 23, 2400 Copenhagen NV, Denmark. abigail.mackey@gmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20930124

Citation

Mackey, Abigail L., et al. "Distribution of Myogenic Progenitor Cells and Myonuclei Is Altered in Women With Vs. Those Without Chronically Painful Trapezius Muscle." Journal of Applied Physiology (Bethesda, Md. : 1985), vol. 109, no. 6, 2010, pp. 1920-9.
Mackey AL, Andersen LL, Frandsen U, et al. Distribution of myogenic progenitor cells and myonuclei is altered in women with vs. those without chronically painful trapezius muscle. J Appl Physiol (1985). 2010;109(6):1920-9.
Mackey, A. L., Andersen, L. L., Frandsen, U., Suetta, C., & Sjøgaard, G. (2010). Distribution of myogenic progenitor cells and myonuclei is altered in women with vs. those without chronically painful trapezius muscle. Journal of Applied Physiology (Bethesda, Md. : 1985), 109(6), 1920-9. https://doi.org/10.1152/japplphysiol.00789.2010
Mackey AL, et al. Distribution of Myogenic Progenitor Cells and Myonuclei Is Altered in Women With Vs. Those Without Chronically Painful Trapezius Muscle. J Appl Physiol (1985). 2010;109(6):1920-9. PubMed PMID: 20930124.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Distribution of myogenic progenitor cells and myonuclei is altered in women with vs. those without chronically painful trapezius muscle. AU - Mackey,Abigail L, AU - Andersen,Lars L, AU - Frandsen,Ulrik, AU - Suetta,Charlotte, AU - Sjøgaard,Gisela, Y1 - 2010/10/07/ PY - 2010/10/9/entrez PY - 2010/10/12/pubmed PY - 2011/3/26/medline SP - 1920 EP - 9 JF - Journal of applied physiology (Bethesda, Md. : 1985) JO - J Appl Physiol (1985) VL - 109 IS - 6 N2 - It is hypothesized that repeated recruitment of low-threshold motor units is an underlying cause of chronic pain in trapezius myalgia. This study investigated the distribution of satellite cells (SCs), myonuclei, and macrophages in muscle biopsies from the trapezius muscle of 42 women performing repetitive manual work, diagnosed with trapezius myalgia (MYA; 44 ± 8 yr; mean ± SD) and 20 matched healthy controls (CON; 45 ± 9 yr). Our hypothesis was that muscle of MYA, in particular type I fibers, would demonstrate higher numbers of SCs, myonuclei, and macrophages compared with CON. SCs were identified on muscle cross sections by combined immunohistochemical staining for Pax7, type I myosin, and laminin, allowing the number of SCs associated with type I and II fibers to be determined. We observed a pattern of SC distribution in MYA previously only reported for individuals above 70 yr of age. Compared with CON, MYA demonstrated 19% more SCs per fiber associated with type I fibers (MYA 0.098 ± 0.039 vs. CON 0.079 ± 0.031; P < 0.05) and 40% fewer SCs associated with type II fibers (MYA 0.047 ± 0.017 vs. CON 0.066 ± 0.035; P < 0.05). The finding of similar numbers of macrophages between the two groups was not in line with our hypothesis and suggests that the elevated SC content of MYA was not due to heightened inflammatory cell contents, but rather to provide new myonuclei. The findings of greater numbers of SCs in type I fibers of muscle subjected to repeated low-intensity work support our hypothesis and provide new insight into stimuli capable of regulating SC content. SN - 1522-1601 UR - https://www.unboundmedicine.com/medline/citation/20930124/Distribution_of_myogenic_progenitor_cells_and_myonuclei_is_altered_in_women_with_vs__those_without_chronically_painful_trapezius_muscle_ L2 - https://journals.physiology.org/doi/10.1152/japplphysiol.00789.2010?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -