Tags

Type your tag names separated by a space and hit enter

Redox proteomics analysis of brains from subjects with amnestic mild cognitive impairment compared to brains from subjects with preclinical Alzheimer's disease: insights into memory loss in MCI.
J Alzheimers Dis. 2011; 23(2):257-69.JA

Abstract

Alzheimer's disease (AD) is a central nervous system disorder pathologically characterized by senile plaques, neurofibrillary tangles, and synapse loss. A small percentage of individuals with normal antemortem psychometric scores, after adjustments for age and education, meet the neuropathological criteria for amnestic mild cognitive impairment (MCI) or AD; these individuals have been termed 'preclinical' or 'asymptomatic' AD (PCAD). In this study, we employed the immunochemical slot-blot method and two-dimensional gel-based redox proteomics to observe differences in protein levels and oxidative modifications between groups with equal levels of AD pathology who differ in regards to clinical symptoms of memory impairment. Results of global oxidative stress measurements revealed significantly higher levels of protein carbonyls in the MCI inferior parietal lobule (IPL) relative to PCAD (and controls), despite equal levels of neuropathology. Proteomics analysis of the IPL revealed differences in protein levels and specific carbonylation that are consistent with preservation of memory in PCAD and apparent memory decline in MCI. Our data suggest that marked changes occur at the protein level in MCI that may cause or reflect memory loss and other AD symptoms.

Authors+Show Affiliations

Department of Chemistry, University of Kentucky, Lexington, KY 40506, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

20930294

Citation

Aluise, Christopher D., et al. "Redox Proteomics Analysis of Brains From Subjects With Amnestic Mild Cognitive Impairment Compared to Brains From Subjects With Preclinical Alzheimer's Disease: Insights Into Memory Loss in MCI." Journal of Alzheimer's Disease : JAD, vol. 23, no. 2, 2011, pp. 257-69.
Aluise CD, Robinson RA, Cai J, et al. Redox proteomics analysis of brains from subjects with amnestic mild cognitive impairment compared to brains from subjects with preclinical Alzheimer's disease: insights into memory loss in MCI. J Alzheimers Dis. 2011;23(2):257-69.
Aluise, C. D., Robinson, R. A., Cai, J., Pierce, W. M., Markesbery, W. R., & Butterfield, D. A. (2011). Redox proteomics analysis of brains from subjects with amnestic mild cognitive impairment compared to brains from subjects with preclinical Alzheimer's disease: insights into memory loss in MCI. Journal of Alzheimer's Disease : JAD, 23(2), 257-69. https://doi.org/10.3233/JAD-2010-101083
Aluise CD, et al. Redox Proteomics Analysis of Brains From Subjects With Amnestic Mild Cognitive Impairment Compared to Brains From Subjects With Preclinical Alzheimer's Disease: Insights Into Memory Loss in MCI. J Alzheimers Dis. 2011;23(2):257-69. PubMed PMID: 20930294.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Redox proteomics analysis of brains from subjects with amnestic mild cognitive impairment compared to brains from subjects with preclinical Alzheimer's disease: insights into memory loss in MCI. AU - Aluise,Christopher D, AU - Robinson,Renã A S, AU - Cai,Jian, AU - Pierce,William M, AU - Markesbery,William R, AU - Butterfield,D Allan, PY - 2010/10/9/entrez PY - 2010/10/12/pubmed PY - 2011/5/13/medline SP - 257 EP - 69 JF - Journal of Alzheimer's disease : JAD JO - J. Alzheimers Dis. VL - 23 IS - 2 N2 - Alzheimer's disease (AD) is a central nervous system disorder pathologically characterized by senile plaques, neurofibrillary tangles, and synapse loss. A small percentage of individuals with normal antemortem psychometric scores, after adjustments for age and education, meet the neuropathological criteria for amnestic mild cognitive impairment (MCI) or AD; these individuals have been termed 'preclinical' or 'asymptomatic' AD (PCAD). In this study, we employed the immunochemical slot-blot method and two-dimensional gel-based redox proteomics to observe differences in protein levels and oxidative modifications between groups with equal levels of AD pathology who differ in regards to clinical symptoms of memory impairment. Results of global oxidative stress measurements revealed significantly higher levels of protein carbonyls in the MCI inferior parietal lobule (IPL) relative to PCAD (and controls), despite equal levels of neuropathology. Proteomics analysis of the IPL revealed differences in protein levels and specific carbonylation that are consistent with preservation of memory in PCAD and apparent memory decline in MCI. Our data suggest that marked changes occur at the protein level in MCI that may cause or reflect memory loss and other AD symptoms. SN - 1875-8908 UR - https://www.unboundmedicine.com/medline/citation/20930294/Redox_proteomics_analysis_of_brains_from_subjects_with_amnestic_mild_cognitive_impairment_compared_to_brains_from_subjects_with_preclinical_Alzheimer's_disease:_insights_into_memory_loss_in_MCI_ L2 - https://content.iospress.com/openurl?genre=article&id=doi:10.3233/JAD-2010-101083 DB - PRIME DP - Unbound Medicine ER -