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Protective effect of edaravone against renal ischemia/reperfusion injury and compared with ischemic postconditioning in rats.
Yao Xue Xue Bao. 2010 Jul; 45(7):840-8.YX

Abstract

The aim of this study is to clarify whether edaravone postconditioning had protective effect against renal ischemia/reperfusion injury and to compare the protective effect between ischemic postconditioning and edaravone postconditioning. Rats were subjected to 45 min ischemia followed by 24 h reperfusion. The rats were randomly assigned to seven groups: a sham-operated control group, an ischemia/reperfusion group, an ischemic postconditioning group, a normal saline vehicle postconditioning group and an edaravone postconditioning (1, 3, and 6 mg x kg(-1)) group. Renal function was assessed by serum creatinine and BUN concentration, while histological damage of renal tissue was assessed with HE staining. MDA content and SOD activity of renal tissue were determined. TUNEL staining was performed to analyze the apoptosis of the tubular epithelial cells, the protein level of Bcl-2 and Bax in renal tissue was examined by Western blotting. Compared to the ischemia/reperfusion group, edaravone postconditioning significantly decreased serum creatinine and BUN concentration, and ameliorated histological damage of renal tissue. MDA was less after 24 h reperfusion in the edaravone postconditioning group than that in the ischemia/reperfusion group, consistent with an increase in SOD activity. In addition, edaravone postconditioning decreased TUNEL-positive cells and Bax expression, and increased Bcl-2 expression. Results detected in the edaravone postconditioning group showed no significant difference from the ischemic postconditioning group. Edaravone administered during the last 3 min of ischemia, prior to reperfusion induces a pharmacological postconditioning in vivo against renal ischemia/reperfusion injury in rats. This protection is similar to that observed with ischemic postconditioning.

Authors+Show Affiliations

Department of Pharmacology, Xuzhou Medical College, Xuzhou 221002, China.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20931780

Citation

Li, Yan, et al. "Protective Effect of Edaravone Against Renal Ischemia/reperfusion Injury and Compared With Ischemic Postconditioning in Rats." Yao Xue Xue Bao = Acta Pharmaceutica Sinica, vol. 45, no. 7, 2010, pp. 840-8.
Li Y, Xia AZ, Xing SH. Protective effect of edaravone against renal ischemia/reperfusion injury and compared with ischemic postconditioning in rats. Yao Xue Xue Bao. 2010;45(7):840-8.
Li, Y., Xia, A. Z., & Xing, S. H. (2010). Protective effect of edaravone against renal ischemia/reperfusion injury and compared with ischemic postconditioning in rats. Yao Xue Xue Bao = Acta Pharmaceutica Sinica, 45(7), 840-8.
Li Y, Xia AZ, Xing SH. Protective Effect of Edaravone Against Renal Ischemia/reperfusion Injury and Compared With Ischemic Postconditioning in Rats. Yao Xue Xue Bao. 2010;45(7):840-8. PubMed PMID: 20931780.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effect of edaravone against renal ischemia/reperfusion injury and compared with ischemic postconditioning in rats. AU - Li,Yan, AU - Xia,An-zhou, AU - Xing,Shu-hua, PY - 2010/10/12/entrez PY - 2010/10/12/pubmed PY - 2012/8/14/medline SP - 840 EP - 8 JF - Yao xue xue bao = Acta pharmaceutica Sinica JO - Yao Xue Xue Bao VL - 45 IS - 7 N2 - The aim of this study is to clarify whether edaravone postconditioning had protective effect against renal ischemia/reperfusion injury and to compare the protective effect between ischemic postconditioning and edaravone postconditioning. Rats were subjected to 45 min ischemia followed by 24 h reperfusion. The rats were randomly assigned to seven groups: a sham-operated control group, an ischemia/reperfusion group, an ischemic postconditioning group, a normal saline vehicle postconditioning group and an edaravone postconditioning (1, 3, and 6 mg x kg(-1)) group. Renal function was assessed by serum creatinine and BUN concentration, while histological damage of renal tissue was assessed with HE staining. MDA content and SOD activity of renal tissue were determined. TUNEL staining was performed to analyze the apoptosis of the tubular epithelial cells, the protein level of Bcl-2 and Bax in renal tissue was examined by Western blotting. Compared to the ischemia/reperfusion group, edaravone postconditioning significantly decreased serum creatinine and BUN concentration, and ameliorated histological damage of renal tissue. MDA was less after 24 h reperfusion in the edaravone postconditioning group than that in the ischemia/reperfusion group, consistent with an increase in SOD activity. In addition, edaravone postconditioning decreased TUNEL-positive cells and Bax expression, and increased Bcl-2 expression. Results detected in the edaravone postconditioning group showed no significant difference from the ischemic postconditioning group. Edaravone administered during the last 3 min of ischemia, prior to reperfusion induces a pharmacological postconditioning in vivo against renal ischemia/reperfusion injury in rats. This protection is similar to that observed with ischemic postconditioning. SN - 0513-4870 UR - https://www.unboundmedicine.com/medline/citation/20931780/Protective_effect_of_edaravone_against_renal_ischemia/reperfusion_injury_and_compared_with_ischemic_postconditioning_in_rats_ DB - PRIME DP - Unbound Medicine ER -