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Atp4b promoter directs the expression of Cre recombinase in gastric parietal cells of transgenic mice.
J Genet Genomics. 2010 Sep; 37(9):647-52.JG

Abstract

Parietal cells are one of the largest epithelium cells of the mucous membrane of the stomach that secrete hydrochloric acid. To study the function of gastric parietal cells during gastric epithelium homeostasis, we generated a transgenic mouse line, namely, Atp4b-Cre, in which the expression of Cre recombinase was controlled by a 1.0 kb promoter of mouse (-subunit of H(+)-, K(+)-ATPase gene (Atp4b). In order to test the tissue distribution and excision activity of Cre recombinase in vivo, the Atp4b-Cre transgenic mice were bred with the reporter strain ROSA26 and a mouse strain that carries Smad4 conditional alleles (Smad4(Co/Co)). Multiple-tissue PCR of Atp4b-Cre;Smad4(Co/+) mice revealed that the recombination only happened in the stomach. As indicated by LacZ staining, ROSA26;Atp4b-Cre double transgenic mice showed efficient expression of Cre recombinase within the gastric parietal cells. These results showed that this Atp4b-Cre mouse line could be used as a powerful tool to achieve conditional gene knockout in gastric parietal cells.

Authors+Show Affiliations

Genetic Laboratory of Development and Diseases, State Key Laboratory of Proteomics, Institute of Biotechnology, Beijing 100071, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20933217

Citation

Zhao, Zengming, et al. "Atp4b Promoter Directs the Expression of Cre Recombinase in Gastric Parietal Cells of Transgenic Mice." Journal of Genetics and Genomics = Yi Chuan Xue Bao, vol. 37, no. 9, 2010, pp. 647-52.
Zhao Z, Hou N, Sun Y, et al. Atp4b promoter directs the expression of Cre recombinase in gastric parietal cells of transgenic mice. J Genet Genomics. 2010;37(9):647-52.
Zhao, Z., Hou, N., Sun, Y., Teng, Y., & Yang, X. (2010). Atp4b promoter directs the expression of Cre recombinase in gastric parietal cells of transgenic mice. Journal of Genetics and Genomics = Yi Chuan Xue Bao, 37(9), 647-52. https://doi.org/10.1016/S1673-8527(09)60083-7
Zhao Z, et al. Atp4b Promoter Directs the Expression of Cre Recombinase in Gastric Parietal Cells of Transgenic Mice. J Genet Genomics. 2010;37(9):647-52. PubMed PMID: 20933217.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Atp4b promoter directs the expression of Cre recombinase in gastric parietal cells of transgenic mice. AU - Zhao,Zengming, AU - Hou,Ning, AU - Sun,Yanxun, AU - Teng,Yan, AU - Yang,Xiao, PY - 2010/04/20/received PY - 2010/08/02/revised PY - 2010/08/03/accepted PY - 2010/10/12/entrez PY - 2010/10/12/pubmed PY - 2011/4/19/medline SP - 647 EP - 52 JF - Journal of genetics and genomics = Yi chuan xue bao JO - J Genet Genomics VL - 37 IS - 9 N2 - Parietal cells are one of the largest epithelium cells of the mucous membrane of the stomach that secrete hydrochloric acid. To study the function of gastric parietal cells during gastric epithelium homeostasis, we generated a transgenic mouse line, namely, Atp4b-Cre, in which the expression of Cre recombinase was controlled by a 1.0 kb promoter of mouse (-subunit of H(+)-, K(+)-ATPase gene (Atp4b). In order to test the tissue distribution and excision activity of Cre recombinase in vivo, the Atp4b-Cre transgenic mice were bred with the reporter strain ROSA26 and a mouse strain that carries Smad4 conditional alleles (Smad4(Co/Co)). Multiple-tissue PCR of Atp4b-Cre;Smad4(Co/+) mice revealed that the recombination only happened in the stomach. As indicated by LacZ staining, ROSA26;Atp4b-Cre double transgenic mice showed efficient expression of Cre recombinase within the gastric parietal cells. These results showed that this Atp4b-Cre mouse line could be used as a powerful tool to achieve conditional gene knockout in gastric parietal cells. SN - 1673-8527 UR - https://www.unboundmedicine.com/medline/citation/20933217/Atp4b_promoter_directs_the_expression_of_Cre_recombinase_in_gastric_parietal_cells_of_transgenic_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1673-8527(09)60083-7 DB - PRIME DP - Unbound Medicine ER -