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Electrospun chitosan-graft-poly (ε -caprolactone)/poly (ε-caprolactone) cationic nanofibrous mats as potential scaffolds for skin tissue engineering.
Int J Biol Macromol 2011; 48(1):13-9IJ

Abstract

This research is aimed to develop cationic nanofibrous mats with improved cellular adhesion profiles and stability of three-dimensional fibrous structure as potential scaffolds for skin tissue engineering. Firstly, amino-remained chitosan-graft-poly (ɛ-caprolactone) (CS-g-PCL) was synthesized with a facile one-step manner by grafting ɛ-caprolactone oligomers onto the hydroxyl groups of CS via ring-opening polymerization by using methanesulfonic acid as solvent and catalyst. And then, CS-g-PCL/PCL nanofibrous mats were obtained by electrospinning of CS-g-PCL/PCL mixed solution. Scanning electron microscopy (SEM) images showed that the morphologies and diameters of the nanofibers were mainly affected by the weight ratio of CS-g-PCL to PCL. The enrichment of amino groups on the nanofiber surface was confirmed by X-ray photoelectron spectroscopy (XPS). With the increase of CS-g-PCL in CS-g-PCL/PCL nanofiber, the content of amino groups on the nanofiber surface increased, which resulted in the increase of zeta-potential of nanofibers. Studies on cell-scaffold interaction were carried out by culturing mouse fibroblast cells (L929) on CS-g-PCL/PCL nanofibrous mats with various contents of CS-g-PCL by assessing the growth, proliferation and morphologies of cells. The results of MTS assay and SEM observation showed that CS-g-PCL/PCL (2/8) mats with a moderate surface zeta-potential (ζ=3mV) were the best in promoting the cell attachment and proliferation. Toluidine blue staining further confirmed that L929 cells grew well and exhibited a normal morphology on the CS-g-PCL/PCL (2/8) mats. These results suggested the potential utilization of CS-g-PCL/PCL (2/8) nanofibrous mats for skin tissue engineering.

Authors+Show Affiliations

Institutes for Advanced Interdisciplinary Research, East China Normal University, No. 3663, North Zhongshan Rd., Shanghai 200062, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20933540

Citation

Chen, Honglin, et al. "Electrospun Chitosan-graft-poly (ε -caprolactone)/poly (ε-caprolactone) Cationic Nanofibrous Mats as Potential Scaffolds for Skin Tissue Engineering." International Journal of Biological Macromolecules, vol. 48, no. 1, 2011, pp. 13-9.
Chen H, Huang J, Yu J, et al. Electrospun chitosan-graft-poly (ε -caprolactone)/poly (ε-caprolactone) cationic nanofibrous mats as potential scaffolds for skin tissue engineering. Int J Biol Macromol. 2011;48(1):13-9.
Chen, H., Huang, J., Yu, J., Liu, S., & Gu, P. (2011). Electrospun chitosan-graft-poly (ε -caprolactone)/poly (ε-caprolactone) cationic nanofibrous mats as potential scaffolds for skin tissue engineering. International Journal of Biological Macromolecules, 48(1), pp. 13-9. doi:10.1016/j.ijbiomac.2010.09.019.
Chen H, et al. Electrospun Chitosan-graft-poly (ε -caprolactone)/poly (ε-caprolactone) Cationic Nanofibrous Mats as Potential Scaffolds for Skin Tissue Engineering. Int J Biol Macromol. 2011 Jan 1;48(1):13-9. PubMed PMID: 20933540.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Electrospun chitosan-graft-poly (ε -caprolactone)/poly (ε-caprolactone) cationic nanofibrous mats as potential scaffolds for skin tissue engineering. AU - Chen,Honglin, AU - Huang,Jin, AU - Yu,Jiahui, AU - Liu,Shiyuan, AU - Gu,Ping, Y1 - 2010/10/08/ PY - 2010/07/22/received PY - 2010/09/07/revised PY - 2010/09/10/accepted PY - 2010/10/12/entrez PY - 2010/10/12/pubmed PY - 2011/4/5/medline SP - 13 EP - 9 JF - International journal of biological macromolecules JO - Int. J. Biol. Macromol. VL - 48 IS - 1 N2 - This research is aimed to develop cationic nanofibrous mats with improved cellular adhesion profiles and stability of three-dimensional fibrous structure as potential scaffolds for skin tissue engineering. Firstly, amino-remained chitosan-graft-poly (ɛ-caprolactone) (CS-g-PCL) was synthesized with a facile one-step manner by grafting ɛ-caprolactone oligomers onto the hydroxyl groups of CS via ring-opening polymerization by using methanesulfonic acid as solvent and catalyst. And then, CS-g-PCL/PCL nanofibrous mats were obtained by electrospinning of CS-g-PCL/PCL mixed solution. Scanning electron microscopy (SEM) images showed that the morphologies and diameters of the nanofibers were mainly affected by the weight ratio of CS-g-PCL to PCL. The enrichment of amino groups on the nanofiber surface was confirmed by X-ray photoelectron spectroscopy (XPS). With the increase of CS-g-PCL in CS-g-PCL/PCL nanofiber, the content of amino groups on the nanofiber surface increased, which resulted in the increase of zeta-potential of nanofibers. Studies on cell-scaffold interaction were carried out by culturing mouse fibroblast cells (L929) on CS-g-PCL/PCL nanofibrous mats with various contents of CS-g-PCL by assessing the growth, proliferation and morphologies of cells. The results of MTS assay and SEM observation showed that CS-g-PCL/PCL (2/8) mats with a moderate surface zeta-potential (ζ=3mV) were the best in promoting the cell attachment and proliferation. Toluidine blue staining further confirmed that L929 cells grew well and exhibited a normal morphology on the CS-g-PCL/PCL (2/8) mats. These results suggested the potential utilization of CS-g-PCL/PCL (2/8) nanofibrous mats for skin tissue engineering. SN - 1879-0003 UR - https://www.unboundmedicine.com/medline/citation/20933540/Electrospun_chitosan_graft_poly__ε__caprolactone_/poly__ε_caprolactone__cationic_nanofibrous_mats_as_potential_scaffolds_for_skin_tissue_engineering_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0141-8130(10)00299-0 DB - PRIME DP - Unbound Medicine ER -