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Development of a sequence-characterized amplified region marker-targeted quantitative PCR assay for strain-specific detection of Oenococcus oeni during wine malolactic fermentation.
Appl Environ Microbiol. 2010 Dec; 76(23):7765-74.AE

Abstract

Control over malolactic fermentation (MLF) is a difficult goal in winemaking and needs rapid methods to monitor Oenococcus oeni malolactic starters (MLS) in a stressful environment such as wine. In this study, we describe a novel quantitative PCR (QPCR) assay enabling the detection of an O. oeni strain during MLF without culturing. O. oeni strain LB221 was used as a model to develop a strain-specific sequence-characterized amplified region (SCAR) marker derived from a discriminatory OPA20-based randomly amplified polymorphic DNA (RAPD) band. The 5' and 3' flanking regions and the copy number of the SCAR marker were characterized using inverse PCR and Southern blotting, respectively. Primer pairs targeting the SCAR sequence enabled strain-specific detection without cross amplification of other O. oeni strains or wine species of lactic acid bacteria (LAB), acetic acid bacteria (AAB), and yeasts. The SCAR-QPCR assay was linear over a range of cell concentrations (7 log units) and detected as few as 2.2 × 10(2) CFU per ml of red wine with good quantification effectiveness, as shown by the correlation of QPCR and plate counting results. Therefore, the cultivation-independent monitoring of a single O. oeni strain in wine based on a SCAR marker represents a rapid and effective strain-specific approach. This strategy can be adopted to develop easy and rapid detection techniques for monitoring the implantation of inoculated O. oeni MLS on the indigenous LAB population, reducing the risk of unsuccessful MLF.

Authors+Show Affiliations

Department of Agricultural and Food Sciences, University of Modena and Reggio Emilia, Via Amendola 2, Padiglione Besta, 42100 Reggio Emilia, Italy. lisa.solieri@unimore.itNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

20935116

Citation

Solieri, Lisa, and Paolo Giudici. "Development of a Sequence-characterized Amplified Region Marker-targeted Quantitative PCR Assay for Strain-specific Detection of Oenococcus Oeni During Wine Malolactic Fermentation." Applied and Environmental Microbiology, vol. 76, no. 23, 2010, pp. 7765-74.
Solieri L, Giudici P. Development of a sequence-characterized amplified region marker-targeted quantitative PCR assay for strain-specific detection of Oenococcus oeni during wine malolactic fermentation. Appl Environ Microbiol. 2010;76(23):7765-74.
Solieri, L., & Giudici, P. (2010). Development of a sequence-characterized amplified region marker-targeted quantitative PCR assay for strain-specific detection of Oenococcus oeni during wine malolactic fermentation. Applied and Environmental Microbiology, 76(23), 7765-74. https://doi.org/10.1128/AEM.00929-10
Solieri L, Giudici P. Development of a Sequence-characterized Amplified Region Marker-targeted Quantitative PCR Assay for Strain-specific Detection of Oenococcus Oeni During Wine Malolactic Fermentation. Appl Environ Microbiol. 2010;76(23):7765-74. PubMed PMID: 20935116.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of a sequence-characterized amplified region marker-targeted quantitative PCR assay for strain-specific detection of Oenococcus oeni during wine malolactic fermentation. AU - Solieri,Lisa, AU - Giudici,Paolo, Y1 - 2010/10/08/ PY - 2010/10/12/entrez PY - 2010/10/12/pubmed PY - 2011/2/26/medline SP - 7765 EP - 74 JF - Applied and environmental microbiology JO - Appl. Environ. Microbiol. VL - 76 IS - 23 N2 - Control over malolactic fermentation (MLF) is a difficult goal in winemaking and needs rapid methods to monitor Oenococcus oeni malolactic starters (MLS) in a stressful environment such as wine. In this study, we describe a novel quantitative PCR (QPCR) assay enabling the detection of an O. oeni strain during MLF without culturing. O. oeni strain LB221 was used as a model to develop a strain-specific sequence-characterized amplified region (SCAR) marker derived from a discriminatory OPA20-based randomly amplified polymorphic DNA (RAPD) band. The 5' and 3' flanking regions and the copy number of the SCAR marker were characterized using inverse PCR and Southern blotting, respectively. Primer pairs targeting the SCAR sequence enabled strain-specific detection without cross amplification of other O. oeni strains or wine species of lactic acid bacteria (LAB), acetic acid bacteria (AAB), and yeasts. The SCAR-QPCR assay was linear over a range of cell concentrations (7 log units) and detected as few as 2.2 × 10(2) CFU per ml of red wine with good quantification effectiveness, as shown by the correlation of QPCR and plate counting results. Therefore, the cultivation-independent monitoring of a single O. oeni strain in wine based on a SCAR marker represents a rapid and effective strain-specific approach. This strategy can be adopted to develop easy and rapid detection techniques for monitoring the implantation of inoculated O. oeni MLS on the indigenous LAB population, reducing the risk of unsuccessful MLF. SN - 1098-5336 UR - https://www.unboundmedicine.com/medline/citation/20935116/Development_of_a_sequence_characterized_amplified_region_marker_targeted_quantitative_PCR_assay_for_strain_specific_detection_of_Oenococcus_oeni_during_wine_malolactic_fermentation_ L2 - http://aem.asm.org/cgi/pmidlookup?view=long&pmid=20935116 DB - PRIME DP - Unbound Medicine ER -