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Enzyme immunoassay detection of antibodies in canine blastomycosis using Blastomyces dermatitidis lysate antigens.
Mycoses. 1990 Sep-Oct; 33(9-10):483-9.M

Abstract

A Blastomyces dermatitidis yeast phase lysate antigen (T-58) prepared in our laboratory was used in an enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies in 201 serum specimens from dogs with blastomycosis. In addition 36 sera from non-infected dogs from an endemic area for blastomycosis and 36 sera from non-infected dogs residing in a blastomycosis non-endemic area were assayed. Sensitivity values ranged from 96.2% (53 pre-treatment sera) to 88.5% (148 post-treatment sera) with the specimens from dogs with confirmed blastomycosis. Minimal reactivity was experienced with the sera from non-infected dogs. Positive reactions were obtained with 8.3% of the endemic area sera and with 5.6% of the sera from the non-endemic area. Mean ELISA index values ranged from 7.66 with the pretreatment sera to 1.11 and 1.03 with the endemic area sera and non-endemic area specimens respectively.

Authors+Show Affiliations

Seawell BW
Department of Biological Sciences, Idaho State University, Pocatello.
Legendre AM
No affiliation info available
Scalarone GM
No affiliation info available

MeSH

AnimalsAntibodies, FungalAntigens, FungalBlastomycesBlastomycosisDog DiseasesDogsEnzyme-Linked Immunosorbent AssaySensitivity and Specificity

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2093845

Citation

Seawell, B W., et al. "Enzyme Immunoassay Detection of Antibodies in Canine Blastomycosis Using Blastomyces Dermatitidis Lysate Antigens." Mycoses, vol. 33, no. 9-10, 1990, pp. 483-9.
Seawell BW, Legendre AM, Scalarone GM. Enzyme immunoassay detection of antibodies in canine blastomycosis using Blastomyces dermatitidis lysate antigens. Mycoses. 1990;33(9-10):483-9.
Seawell, B. W., Legendre, A. M., & Scalarone, G. M. (1990). Enzyme immunoassay detection of antibodies in canine blastomycosis using Blastomyces dermatitidis lysate antigens. Mycoses, 33(9-10), 483-9.
Seawell BW, Legendre AM, Scalarone GM. Enzyme Immunoassay Detection of Antibodies in Canine Blastomycosis Using Blastomyces Dermatitidis Lysate Antigens. Mycoses. 1990 Sep-Oct;33(9-10):483-9. PubMed PMID: 2093845.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enzyme immunoassay detection of antibodies in canine blastomycosis using Blastomyces dermatitidis lysate antigens. AU - Seawell,B W, AU - Legendre,A M, AU - Scalarone,G M, PY - 1990/9/1/pubmed PY - 1990/9/1/medline PY - 1990/9/1/entrez SP - 483 EP - 9 JF - Mycoses JO - Mycoses VL - 33 IS - 9-10 N2 - A Blastomyces dermatitidis yeast phase lysate antigen (T-58) prepared in our laboratory was used in an enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies in 201 serum specimens from dogs with blastomycosis. In addition 36 sera from non-infected dogs from an endemic area for blastomycosis and 36 sera from non-infected dogs residing in a blastomycosis non-endemic area were assayed. Sensitivity values ranged from 96.2% (53 pre-treatment sera) to 88.5% (148 post-treatment sera) with the specimens from dogs with confirmed blastomycosis. Minimal reactivity was experienced with the sera from non-infected dogs. Positive reactions were obtained with 8.3% of the endemic area sera and with 5.6% of the sera from the non-endemic area. Mean ELISA index values ranged from 7.66 with the pretreatment sera to 1.11 and 1.03 with the endemic area sera and non-endemic area specimens respectively. SN - 0933-7407 UR - https://www.unboundmedicine.com/medline/citation/2093845/Enzyme_immunoassay_detection_of_antibodies_in_canine_blastomycosis_using_Blastomyces_dermatitidis_lysate_antigens_ DB - PRIME DP - Unbound Medicine ER -