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Effects of insulin-like growth factor-1 on expression of sensory neuropeptides in cultured dorsal root ganglion neurons in the absence or presence of glutamate.
Int J Neurosci. 2010 Nov; 120(11):698-702.IJ

Abstract

Insulin-like growth factor-1 (IGF-1) is a neurotrophic factor and plays an important role in promoting axonal growth from dorsal root ganglion (DRG) neurons. Whether IGF-1 could influence expression of sensory neuropeptides substance P (SP) and calcitonin gene-related peptide (CGRP) of the cultured DRG neurons with excitotoxicity induced by glutamate (Glu) remains unknown. In the present study, primary cultured DRG neurons were used to determine the effects of IGF-1 on expression of SP and CGRP of the neurons with Glu-induced excitotoxicity. The DRG neurons were dissociated and cultured for 48 hr and then exposed to Glu (0.2 mmol/L), IGF-1 (20 nmol/L), and Glu (0.2 mmol/L) plus IGF-1 (20 nmol/L) for additional 12 hr. The DRG neurons were continuously exposed to growth media as control. After that, all the above cultured DRG neurons were processed for detecting SP and CGRP expression by Western blot analysis. The expression of SP and CGRP increased significantly in primary cultured DRG neurons in the presence of IGF-1. The ability of IGF-1 on SP and CGRP expression may play a role in neurogenic inflammation or nociception.

Authors+Show Affiliations

Department of Anatomy, Shandong University School of Medicine, Jinan, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20942583

Citation

Liu, Zhen, et al. "Effects of Insulin-like Growth Factor-1 On Expression of Sensory Neuropeptides in Cultured Dorsal Root Ganglion Neurons in the Absence or Presence of Glutamate." The International Journal of Neuroscience, vol. 120, no. 11, 2010, pp. 698-702.
Liu Z, Liu H, Yang X, et al. Effects of insulin-like growth factor-1 on expression of sensory neuropeptides in cultured dorsal root ganglion neurons in the absence or presence of glutamate. Int J Neurosci. 2010;120(11):698-702.
Liu, Z., Liu, H., Yang, X., Xu, X., Zhang, W., & Li, Z. (2010). Effects of insulin-like growth factor-1 on expression of sensory neuropeptides in cultured dorsal root ganglion neurons in the absence or presence of glutamate. The International Journal of Neuroscience, 120(11), 698-702. https://doi.org/10.3109/00207454.2010.513463
Liu Z, et al. Effects of Insulin-like Growth Factor-1 On Expression of Sensory Neuropeptides in Cultured Dorsal Root Ganglion Neurons in the Absence or Presence of Glutamate. Int J Neurosci. 2010;120(11):698-702. PubMed PMID: 20942583.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of insulin-like growth factor-1 on expression of sensory neuropeptides in cultured dorsal root ganglion neurons in the absence or presence of glutamate. AU - Liu,Zhen, AU - Liu,Huaxiang, AU - Yang,Xiangdong, AU - Xu,Xiaofeng, AU - Zhang,Weiwei, AU - Li,Zhenzhong, PY - 2010/10/15/entrez PY - 2010/10/15/pubmed PY - 2011/5/20/medline SP - 698 EP - 702 JF - The International journal of neuroscience JO - Int. J. Neurosci. VL - 120 IS - 11 N2 - Insulin-like growth factor-1 (IGF-1) is a neurotrophic factor and plays an important role in promoting axonal growth from dorsal root ganglion (DRG) neurons. Whether IGF-1 could influence expression of sensory neuropeptides substance P (SP) and calcitonin gene-related peptide (CGRP) of the cultured DRG neurons with excitotoxicity induced by glutamate (Glu) remains unknown. In the present study, primary cultured DRG neurons were used to determine the effects of IGF-1 on expression of SP and CGRP of the neurons with Glu-induced excitotoxicity. The DRG neurons were dissociated and cultured for 48 hr and then exposed to Glu (0.2 mmol/L), IGF-1 (20 nmol/L), and Glu (0.2 mmol/L) plus IGF-1 (20 nmol/L) for additional 12 hr. The DRG neurons were continuously exposed to growth media as control. After that, all the above cultured DRG neurons were processed for detecting SP and CGRP expression by Western blot analysis. The expression of SP and CGRP increased significantly in primary cultured DRG neurons in the presence of IGF-1. The ability of IGF-1 on SP and CGRP expression may play a role in neurogenic inflammation or nociception. SN - 1563-5279 UR - https://www.unboundmedicine.com/medline/citation/20942583/Effects_of_insulin_like_growth_factor_1_on_expression_of_sensory_neuropeptides_in_cultured_dorsal_root_ganglion_neurons_in_the_absence_or_presence_of_glutamate_ L2 - http://www.tandfonline.com/doi/full/10.3109/00207454.2010.513463 DB - PRIME DP - Unbound Medicine ER -