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Colistin-tobramycin combinations are superior to monotherapy concerning the killing of biofilm Pseudomonas aeruginosa.
J Infect Dis. 2010 Nov 15; 202(10):1585-92.JI

Abstract

BACKGROUND

Antibiotic combination therapy might be more efficient than single antibiotics to combat Pseudomonas aeruginosa biofilms in the airways of patients with cystic fibrosis. We tested the ability of colistin sulphate-tobramycin combinations and single antibiotics to kill P. aeruginosa biofilms.

METHODS

P. aeruginosa biofilms were generated in vitro and in rat lungs. In a pilot study, 5 patients with cystic fibrosis inhaled colistin and then tobramycin for 4 weeks. The changes in P. aeruginosa counts and lung function were assessed before and after therapy.

RESULTS

Antibiotic combination therapy significantly reduced the number of P. aeruginosa cells in P. aeruginosa biofilm models in vitro. When rats were challenged with 1 x 10(7) cfu of P. aeruginosa, which was embedded in alginate beads, mortality rates, lung pathologic findings, and bacterial colony-forming unit counts were significantly lower after 7 days in animals receiving antibiotic combination than in animals receiving single antibiotics. In patients with cystic fibrosis, inhaled colistin-tobramycin was well tolerated and resulted in a mean decrease of 2.52 + /- 2.5 log(10) cfu of P. aeruginosa per milliliter of sputum (P = .027). Measurements of forced expiratory volume in 1 s, obtained both before and after the study, did not differ significantly.

CONCLUSION

Colistin-tobramycin combinations are more efficient than respective single antibiotics for killing P. aeruginosa in biofilms in vitro, and they significantly reduced P. aeruginosa cell counts in a rat lung infection model and in patients with cystic fibrosis.

Authors+Show Affiliations

Institute of Medical Microbiology and Hygiene, Universitätsklinikum, University of Tübingen, Tübingen, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20942647

Citation

Herrmann, Gloria, et al. "Colistin-tobramycin Combinations Are Superior to Monotherapy Concerning the Killing of Biofilm Pseudomonas Aeruginosa." The Journal of Infectious Diseases, vol. 202, no. 10, 2010, pp. 1585-92.
Herrmann G, Yang L, Wu H, et al. Colistin-tobramycin combinations are superior to monotherapy concerning the killing of biofilm Pseudomonas aeruginosa. J Infect Dis. 2010;202(10):1585-92.
Herrmann, G., Yang, L., Wu, H., Song, Z., Wang, H., Høiby, N., Ulrich, M., Molin, S., Riethmüller, J., & Döring, G. (2010). Colistin-tobramycin combinations are superior to monotherapy concerning the killing of biofilm Pseudomonas aeruginosa. The Journal of Infectious Diseases, 202(10), 1585-92. https://doi.org/10.1086/656788
Herrmann G, et al. Colistin-tobramycin Combinations Are Superior to Monotherapy Concerning the Killing of Biofilm Pseudomonas Aeruginosa. J Infect Dis. 2010 Nov 15;202(10):1585-92. PubMed PMID: 20942647.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Colistin-tobramycin combinations are superior to monotherapy concerning the killing of biofilm Pseudomonas aeruginosa. AU - Herrmann,Gloria, AU - Yang,Liang, AU - Wu,Hong, AU - Song,Zhijun, AU - Wang,Hengzhuang, AU - Høiby,Niels, AU - Ulrich,Martina, AU - Molin,Søren, AU - Riethmüller,Joachim, AU - Döring,Gerd, Y1 - 2010/10/13/ PY - 2010/10/15/entrez PY - 2010/10/15/pubmed PY - 2010/12/14/medline SP - 1585 EP - 92 JF - The Journal of infectious diseases JO - J Infect Dis VL - 202 IS - 10 N2 - BACKGROUND: Antibiotic combination therapy might be more efficient than single antibiotics to combat Pseudomonas aeruginosa biofilms in the airways of patients with cystic fibrosis. We tested the ability of colistin sulphate-tobramycin combinations and single antibiotics to kill P. aeruginosa biofilms. METHODS: P. aeruginosa biofilms were generated in vitro and in rat lungs. In a pilot study, 5 patients with cystic fibrosis inhaled colistin and then tobramycin for 4 weeks. The changes in P. aeruginosa counts and lung function were assessed before and after therapy. RESULTS: Antibiotic combination therapy significantly reduced the number of P. aeruginosa cells in P. aeruginosa biofilm models in vitro. When rats were challenged with 1 x 10(7) cfu of P. aeruginosa, which was embedded in alginate beads, mortality rates, lung pathologic findings, and bacterial colony-forming unit counts were significantly lower after 7 days in animals receiving antibiotic combination than in animals receiving single antibiotics. In patients with cystic fibrosis, inhaled colistin-tobramycin was well tolerated and resulted in a mean decrease of 2.52 + /- 2.5 log(10) cfu of P. aeruginosa per milliliter of sputum (P = .027). Measurements of forced expiratory volume in 1 s, obtained both before and after the study, did not differ significantly. CONCLUSION: Colistin-tobramycin combinations are more efficient than respective single antibiotics for killing P. aeruginosa in biofilms in vitro, and they significantly reduced P. aeruginosa cell counts in a rat lung infection model and in patients with cystic fibrosis. SN - 1537-6613 UR - https://www.unboundmedicine.com/medline/citation/20942647/Colistin_tobramycin_combinations_are_superior_to_monotherapy_concerning_the_killing_of_biofilm_Pseudomonas_aeruginosa_ L2 - https://academic.oup.com/jid/article-lookup/doi/10.1086/656788 DB - PRIME DP - Unbound Medicine ER -