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[Serum markers of oxidative stress in infertile women with endometriosis].
Rev Bras Ginecol Obstet. 2010 Jun; 32(6):279-85.RB

Abstract

PURPOSE

to compare serum markers of oxidative stress between infertile patients with and without endometriosis and to assess the association of these markers with disease staging.

METHODS

this was a prospective study conducted on 112 consecutive infertile, non-obese patients younger than 39 years, divided into two groups: Endometriosis (n=48, 26 with minimal and mild endometriosis - Stage I/II, and 22 with moderate and severe endometriosis - Stage III/IV) and Control (n=64, with tubal and/or male factor infertility). Blood samples were collected during the early follicular phase of the menstrual cycle for the analysis of serum malondialdehyde, glutathione and total hydroxyperoxide levels by spectrophotometry and of vitamin E by high performance liquid chromatography. The results were compared between the endometriosis and control groups, stage I/II endometriosis and control, stage III/IV endometriosis and control, and between the two endometriosis subgroups. The level of significance was set at 5% (p < 0.05) in all analyses.

RESULTS

vitamin E and glutathione levels were lower in the serum of infertile women with moderate/severe endometriosis (21.7 ± 6.0 mMol/L and 159.6 ± 77.2 nMol/g protein, respectively) compared to women with minimal and mild endometriosis (28.3 ± 14.4 mMol/L and 199.6 ± 56.1 nMol/g protein, respectively). Total hydroxyperoxide levels were significantly higher in the endometriosis group (8.9 ± 1.8 µMol/g protein) than in the Control Group (8.0 ± 2 µMol/g protein) and among patients with stage III/IV disease (9.7 ± 2.3 µMol/g protein) compared to patients with stage I/II disease (8.2 ± 1.0 µMol/g protein). No significant differences in serum malondialdehyde levels were observed between groups.

CONCLUSIONS

we demonstrated a positive association between infertility related to endometriosis, advanced disease stage and increased serum hydroxyperoxide levels, suggesting an increased production of reactive species in women with endometriosis. These data, taken together with the reduction of serum vitamin E and glutathione levels, suggest the occurrence of systemic oxidative stress in women with infertility associated with endometriosis. The reproductive and metabolic implications of oxidative stress should be assessed in future studies.

Authors+Show Affiliations

Departamento de Ginecologia e Obstetrícia, Faculdade de Medicina de Ribeirão Preto, USP, Ribeirão Preto, SP, Brasil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

por

PubMed ID

20945013

Citation

Andrade, Aline Zyman de, et al. "[Serum Markers of Oxidative Stress in Infertile Women With Endometriosis]." Revista Brasileira De Ginecologia E Obstetricia : Revista Da Federacao Brasileira Das Sociedades De Ginecologia E Obstetricia, vol. 32, no. 6, 2010, pp. 279-85.
Andrade AZ, Rodrigues JK, Dib LA, et al. [Serum markers of oxidative stress in infertile women with endometriosis]. Rev Bras Ginecol Obstet. 2010;32(6):279-85.
Andrade, A. Z., Rodrigues, J. K., Dib, L. A., Romão, G. S., Ferriani, R. A., Jordão Junior, A. A., & Navarro, P. A. (2010). [Serum markers of oxidative stress in infertile women with endometriosis]. Revista Brasileira De Ginecologia E Obstetricia : Revista Da Federacao Brasileira Das Sociedades De Ginecologia E Obstetricia, 32(6), 279-85.
Andrade AZ, et al. [Serum Markers of Oxidative Stress in Infertile Women With Endometriosis]. Rev Bras Ginecol Obstet. 2010;32(6):279-85. PubMed PMID: 20945013.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Serum markers of oxidative stress in infertile women with endometriosis]. AU - Andrade,Aline Zyman de, AU - Rodrigues,Jhenifer Kliemchen, AU - Dib,Luciana Azôr, AU - Romão,Gustavo Salata, AU - Ferriani,Rui Alberto, AU - Jordão Junior,Alceu Afonso, AU - Navarro,Paula Andrea de Albuquerque Salles, PY - 2010/05/06/received PY - 2010/05/31/accepted PY - 2010/10/15/entrez PY - 2010/10/15/pubmed PY - 2011/2/25/medline SP - 279 EP - 85 JF - Revista brasileira de ginecologia e obstetricia : revista da Federacao Brasileira das Sociedades de Ginecologia e Obstetricia JO - Rev Bras Ginecol Obstet VL - 32 IS - 6 N2 - PURPOSE: to compare serum markers of oxidative stress between infertile patients with and without endometriosis and to assess the association of these markers with disease staging. METHODS: this was a prospective study conducted on 112 consecutive infertile, non-obese patients younger than 39 years, divided into two groups: Endometriosis (n=48, 26 with minimal and mild endometriosis - Stage I/II, and 22 with moderate and severe endometriosis - Stage III/IV) and Control (n=64, with tubal and/or male factor infertility). Blood samples were collected during the early follicular phase of the menstrual cycle for the analysis of serum malondialdehyde, glutathione and total hydroxyperoxide levels by spectrophotometry and of vitamin E by high performance liquid chromatography. The results were compared between the endometriosis and control groups, stage I/II endometriosis and control, stage III/IV endometriosis and control, and between the two endometriosis subgroups. The level of significance was set at 5% (p < 0.05) in all analyses. RESULTS: vitamin E and glutathione levels were lower in the serum of infertile women with moderate/severe endometriosis (21.7 ± 6.0 mMol/L and 159.6 ± 77.2 nMol/g protein, respectively) compared to women with minimal and mild endometriosis (28.3 ± 14.4 mMol/L and 199.6 ± 56.1 nMol/g protein, respectively). Total hydroxyperoxide levels were significantly higher in the endometriosis group (8.9 ± 1.8 µMol/g protein) than in the Control Group (8.0 ± 2 µMol/g protein) and among patients with stage III/IV disease (9.7 ± 2.3 µMol/g protein) compared to patients with stage I/II disease (8.2 ± 1.0 µMol/g protein). No significant differences in serum malondialdehyde levels were observed between groups. CONCLUSIONS: we demonstrated a positive association between infertility related to endometriosis, advanced disease stage and increased serum hydroxyperoxide levels, suggesting an increased production of reactive species in women with endometriosis. These data, taken together with the reduction of serum vitamin E and glutathione levels, suggest the occurrence of systemic oxidative stress in women with infertility associated with endometriosis. The reproductive and metabolic implications of oxidative stress should be assessed in future studies. SN - 1806-9339 UR - https://www.unboundmedicine.com/medline/citation/20945013/[Serum_markers_of_oxidative_stress_in_infertile_women_with_endometriosis]_ L2 - http://www.diseaseinfosearch.org/result/2537 DB - PRIME DP - Unbound Medicine ER -