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Genome-wide approaches to monitor pre-mRNA splicing.
Methods Enzymol. 2010; 470:51-75.ME

Abstract

Pre-mRNA processing is an essential control-point in the gene expression pathway of eukaryotic organisms. The budding yeast Saccharomyces cerevisiae offers a powerful opportunity to examine the regulation of this pathway. In this chapter, we will describe methods that have been developed in our lab and others to examine pre-mRNA splicing from a genome-wide perspective in yeast. Our goal is to provide all of the necessary information--from microarray design to experimental setup to data analysis--to facilitate the widespread use of this technology.

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Authors+Show Affiliations

Inada M
Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York, USA.
Pleiss JA
No affiliation info available

MeSH

DNA, ComplementaryGenome, FungalOligonucleotide Array Sequence AnalysisRNA PrecursorsRNA SplicingRNA, FungalSaccharomyces cerevisiae

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20946806

Citation

Inada, Maki, and Jeffrey A. Pleiss. "Genome-wide Approaches to Monitor pre-mRNA Splicing." Methods in Enzymology, vol. 470, 2010, pp. 51-75.
Inada M, Pleiss JA. Genome-wide approaches to monitor pre-mRNA splicing. Methods Enzymol. 2010;470:51-75.
Inada, M., & Pleiss, J. A. (2010). Genome-wide approaches to monitor pre-mRNA splicing. Methods in Enzymology, 470, 51-75. https://doi.org/10.1016/S0076-6879(10)70003-3
Inada M, Pleiss JA. Genome-wide Approaches to Monitor pre-mRNA Splicing. Methods Enzymol. 2010;470:51-75. PubMed PMID: 20946806.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genome-wide approaches to monitor pre-mRNA splicing. AU - Inada,Maki, AU - Pleiss,Jeffrey A, Y1 - 2010/03/01/ PY - 2010/10/16/entrez PY - 2010/10/16/pubmed PY - 2011/1/28/medline SP - 51 EP - 75 JF - Methods in enzymology JO - Methods Enzymol VL - 470 N2 - Pre-mRNA processing is an essential control-point in the gene expression pathway of eukaryotic organisms. The budding yeast Saccharomyces cerevisiae offers a powerful opportunity to examine the regulation of this pathway. In this chapter, we will describe methods that have been developed in our lab and others to examine pre-mRNA splicing from a genome-wide perspective in yeast. Our goal is to provide all of the necessary information--from microarray design to experimental setup to data analysis--to facilitate the widespread use of this technology. SN - 1557-7988 UR - https://www.unboundmedicine.com/medline/citation/20946806/Genome_wide_approaches_to_monitor_pre_mRNA_splicing_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0076-6879(10)70003-3 DB - PRIME DP - Unbound Medicine ER -