Tags

Type your tag names separated by a space and hit enter

Selenium in water enhances antioxidant defenses and protects against copper-induced DNA damage in the blue mussel Mytilus edulis.
Aquat Toxicol. 2011 Jan 17; 101(1):64-71.AT

Abstract

Selenium and copper are naturally occurring elements in the environment that have important roles in cellular function. Selenium is known for its role in antioxidant defense, whereas copper is a redox-active metal capable of acting as a pro-oxidant. We investigated the effects of short term selenium (Na(2)SeO(3)) supplementation (4 μg/L for 3 days) on antioxidant parameters of the blue mussel, Mytilus edulis, and its possible protective effects against a subsequent copper (CuSO(4)) exposure (56 μg/L for 3 days). Selenium supplementation caused a 4-fold increase in glutathione levels in gills. The activity of selenium-dependent glutathione peroxidase was modulated by selenium in gills (2-fold increase) and also in cell-free haemolymph (40% increase). Copper exposure produced decreases in protein thiol levels (35%) and in thioredoxin reductase activity (60%) in gills and induced an increase in DNA damage in haemocytes (70% increase in % tail DNA observed using the comet assay). The decrease in thioredoxin reductase activity may constitute a mechanism of copper toxicity in bivalves, warranting further investigation. Pre-treatment with selenium largely prevented these deleterious effects of copper on protein thiols, thioredoxin reductase activity and DNA damage. The results suggest that induction of key antioxidant defenses such as glutathione and selenium-dependent glutathione peroxidase, as a result of selenium supplementation, may play an important role in protection of aquatic organisms against oxidative stress.

Authors+Show Affiliations

Departamento de Bioquímica, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20947183

Citation

Trevisan, Rafael, et al. "Selenium in Water Enhances Antioxidant Defenses and Protects Against Copper-induced DNA Damage in the Blue Mussel Mytilus Edulis." Aquatic Toxicology (Amsterdam, Netherlands), vol. 101, no. 1, 2011, pp. 64-71.
Trevisan R, Mello DF, Fisher AS, et al. Selenium in water enhances antioxidant defenses and protects against copper-induced DNA damage in the blue mussel Mytilus edulis. Aquat Toxicol. 2011;101(1):64-71.
Trevisan, R., Mello, D. F., Fisher, A. S., Schuwerack, P. M., Dafre, A. L., & Moody, A. J. (2011). Selenium in water enhances antioxidant defenses and protects against copper-induced DNA damage in the blue mussel Mytilus edulis. Aquatic Toxicology (Amsterdam, Netherlands), 101(1), 64-71. https://doi.org/10.1016/j.aquatox.2010.09.003
Trevisan R, et al. Selenium in Water Enhances Antioxidant Defenses and Protects Against Copper-induced DNA Damage in the Blue Mussel Mytilus Edulis. Aquat Toxicol. 2011 Jan 17;101(1):64-71. PubMed PMID: 20947183.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Selenium in water enhances antioxidant defenses and protects against copper-induced DNA damage in the blue mussel Mytilus edulis. AU - Trevisan,Rafael, AU - Mello,Danielle Ferraz, AU - Fisher,Andrew S, AU - Schuwerack,Petra-Manuela, AU - Dafre,Alcir Luiz, AU - Moody,A John, Y1 - 2010/09/16/ PY - 2010/07/19/received PY - 2010/08/31/revised PY - 2010/09/04/accepted PY - 2010/10/16/entrez PY - 2010/10/16/pubmed PY - 2011/3/30/medline SP - 64 EP - 71 JF - Aquatic toxicology (Amsterdam, Netherlands) JO - Aquat Toxicol VL - 101 IS - 1 N2 - Selenium and copper are naturally occurring elements in the environment that have important roles in cellular function. Selenium is known for its role in antioxidant defense, whereas copper is a redox-active metal capable of acting as a pro-oxidant. We investigated the effects of short term selenium (Na(2)SeO(3)) supplementation (4 μg/L for 3 days) on antioxidant parameters of the blue mussel, Mytilus edulis, and its possible protective effects against a subsequent copper (CuSO(4)) exposure (56 μg/L for 3 days). Selenium supplementation caused a 4-fold increase in glutathione levels in gills. The activity of selenium-dependent glutathione peroxidase was modulated by selenium in gills (2-fold increase) and also in cell-free haemolymph (40% increase). Copper exposure produced decreases in protein thiol levels (35%) and in thioredoxin reductase activity (60%) in gills and induced an increase in DNA damage in haemocytes (70% increase in % tail DNA observed using the comet assay). The decrease in thioredoxin reductase activity may constitute a mechanism of copper toxicity in bivalves, warranting further investigation. Pre-treatment with selenium largely prevented these deleterious effects of copper on protein thiols, thioredoxin reductase activity and DNA damage. The results suggest that induction of key antioxidant defenses such as glutathione and selenium-dependent glutathione peroxidase, as a result of selenium supplementation, may play an important role in protection of aquatic organisms against oxidative stress. SN - 1879-1514 UR - https://www.unboundmedicine.com/medline/citation/20947183/Selenium_in_water_enhances_antioxidant_defenses_and_protects_against_copper_induced_DNA_damage_in_the_blue_mussel_Mytilus_edulis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-445X(10)00370-X DB - PRIME DP - Unbound Medicine ER -