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Gold coated ferric oxide nanoparticles based disposable magnetic genosensors for the detection of DNA hybridization processes.
Biosens Bioelectron. 2011 Jan 15; 26(5):2194-200.BB

Abstract

In this article, a disposable magnetic DNA sensor using an enzymatic amplification strategy for the detection of specific hybridization processes, based on the coupling of streptavidin-peroxidase to biotinylated target sequences, has been developed. A thiolated 19-mer capture probe was attached to gold coated ferric oxide nanoparticles and hybridization with the biotinylated target was allowed to proceed. Then, a streptavidin-peroxide was attached to the biotinylated target and the resulting modified gold coated ferric oxide nanoparticles were captured by a magnetic field on the surface of a home-made carbon screen printed electrode (SPE). Using hydroquinone as a mediator, a square wave voltammetric procedure was chosen to detect the hybridization process after the addition of hydrogen peroxide. Different aspects concerning the assay protocol and nanoparticles fabrication were optimized in order to improve the sensitivity of the developed methodology. A low detection limit (31 pM) with good stability (RSD=7.04%, n=10) was obtained without the need of polymerase chain reaction (PCR) amplification.

Authors+Show Affiliations

Sensors and Photonics Unit, New Materials Department, CIDETEC-Center for Electrochemical Technologies, Parque Tecnológico de San Sebastián, P° Miramon, 196, E-20009 Donostia-San Sebastián, Guipuzcoa, Spain. oloaiza@cidetec.esNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20951565

Citation

Loaiza, Óscar A., et al. "Gold Coated Ferric Oxide Nanoparticles Based Disposable Magnetic Genosensors for the Detection of DNA Hybridization Processes." Biosensors & Bioelectronics, vol. 26, no. 5, 2011, pp. 2194-200.
Loaiza ÓA, Jubete E, Ochoteco E, et al. Gold coated ferric oxide nanoparticles based disposable magnetic genosensors for the detection of DNA hybridization processes. Biosens Bioelectron. 2011;26(5):2194-200.
Loaiza, Ó. A., Jubete, E., Ochoteco, E., Cabañero, G., Grande, H., & Rodríguez, J. (2011). Gold coated ferric oxide nanoparticles based disposable magnetic genosensors for the detection of DNA hybridization processes. Biosensors & Bioelectronics, 26(5), 2194-200. https://doi.org/10.1016/j.bios.2010.09.034
Loaiza ÓA, et al. Gold Coated Ferric Oxide Nanoparticles Based Disposable Magnetic Genosensors for the Detection of DNA Hybridization Processes. Biosens Bioelectron. 2011 Jan 15;26(5):2194-200. PubMed PMID: 20951565.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gold coated ferric oxide nanoparticles based disposable magnetic genosensors for the detection of DNA hybridization processes. AU - Loaiza,Óscar A, AU - Jubete,Elena, AU - Ochoteco,Estibalitz, AU - Cabañero,German, AU - Grande,Hans, AU - Rodríguez,Javier, Y1 - 2010/09/25/ PY - 2010/07/22/received PY - 2010/09/14/revised PY - 2010/09/18/accepted PY - 2010/10/19/entrez PY - 2010/10/19/pubmed PY - 2011/4/16/medline SP - 2194 EP - 200 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 26 IS - 5 N2 - In this article, a disposable magnetic DNA sensor using an enzymatic amplification strategy for the detection of specific hybridization processes, based on the coupling of streptavidin-peroxidase to biotinylated target sequences, has been developed. A thiolated 19-mer capture probe was attached to gold coated ferric oxide nanoparticles and hybridization with the biotinylated target was allowed to proceed. Then, a streptavidin-peroxide was attached to the biotinylated target and the resulting modified gold coated ferric oxide nanoparticles were captured by a magnetic field on the surface of a home-made carbon screen printed electrode (SPE). Using hydroquinone as a mediator, a square wave voltammetric procedure was chosen to detect the hybridization process after the addition of hydrogen peroxide. Different aspects concerning the assay protocol and nanoparticles fabrication were optimized in order to improve the sensitivity of the developed methodology. A low detection limit (31 pM) with good stability (RSD=7.04%, n=10) was obtained without the need of polymerase chain reaction (PCR) amplification. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/20951565/Gold_coated_ferric_oxide_nanoparticles_based_disposable_magnetic_genosensors_for_the_detection_of_DNA_hybridization_processes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(10)00648-2 DB - PRIME DP - Unbound Medicine ER -