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[Impact of distillage recycling on the glycolysis key enzymes, stress response metabolites and intracelluler components of the self-flocculating yeast].
Sheng Wu Gong Cheng Xue Bao. 2010 Jul; 26(7):1019-24.SW

Abstract

This research aimed to study the effect of distillage recycling on ethanol fermentation, the key glycolytic enzymes and cell composition of the self-flocculating yeast. With the self-flocculating yeast SPSC01 and medium composed of 220 g/L glucose, 8 g/L yeast extract and 6 g/L peptone, continuous ethanol fermentation was carried out at the dilution rate of 0.04 h(-1) with a 1.5 L tank bioreactor. Fermentation broth was collected every 3 days, and ethanol and other volatile byproducts were removed by distillation, but the stillage with high boiling byproducts was recycled to prepare the medium instead of fresh water. The system was run for 20 days, during which ethanol and biomass concentrations in the effluent decreased continuously, indicating the significant inhibition of the high boiling byproducts accumulated within the system. Thus, the activities of the key enzymes of the glycolytic pathway: hexokinase, 6-phosphofructose kinase, and pyruvate kinase were analyzed, and it was observed that all of them were inhibited. Furthermore, the biosynthesis of the stress response metabolites glycerol and trehalose was investigated, and it was found that glycerol production that can protect yeast cells against osmotic pressure stress was enhanced, but trehalose biosynthesis that can protect yeast cells against ethanol inhibition was not improved, correspondingly. And in the meantime, the biosynthesis of the major intracellular components proteins and hydrocarbons was adjusted, correspondingly.

Authors+Show Affiliations

Zi L
School of Bioscience and Bioengineering, Dalian University of Technology, Dalian 116023, China. lihanzi@dlut.edu.cn
Zhang C
No affiliation info available
Ren J
No affiliation info available
Yuan W
No affiliation info available
Chen L
No affiliation info available

MeSH

BioreactorsEthanolFermentationFlocculationGlycerolGlycolysisHexokinaseIndustrial MicrobiologyPhosphofructokinase-1Saccharomyces cerevisiaeSchizosaccharomycesTrehaloseTriticumZea mays

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

20954406

Citation

Zi, Lihan, et al. "[Impact of Distillage Recycling On the Glycolysis Key Enzymes, Stress Response Metabolites and Intracelluler Components of the Self-flocculating Yeast]." Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology, vol. 26, no. 7, 2010, pp. 1019-24.
Zi L, Zhang C, Ren J, et al. [Impact of distillage recycling on the glycolysis key enzymes, stress response metabolites and intracelluler components of the self-flocculating yeast]. Sheng Wu Gong Cheng Xue Bao. 2010;26(7):1019-24.
Zi, L., Zhang, C., Ren, J., Yuan, W., & Chen, L. (2010). [Impact of distillage recycling on the glycolysis key enzymes, stress response metabolites and intracelluler components of the self-flocculating yeast]. Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology, 26(7), 1019-24.
Zi L, et al. [Impact of Distillage Recycling On the Glycolysis Key Enzymes, Stress Response Metabolites and Intracelluler Components of the Self-flocculating Yeast]. Sheng Wu Gong Cheng Xue Bao. 2010;26(7):1019-24. PubMed PMID: 20954406.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Impact of distillage recycling on the glycolysis key enzymes, stress response metabolites and intracelluler components of the self-flocculating yeast]. AU - Zi,Lihan, AU - Zhang,Chunming, AU - Ren,Jiangang, AU - Yuan,Wenjie, AU - Chen,Lijie, PY - 2010/10/20/entrez PY - 2010/10/20/pubmed PY - 2011/9/29/medline SP - 1019 EP - 24 JF - Sheng wu gong cheng xue bao = Chinese journal of biotechnology JO - Sheng Wu Gong Cheng Xue Bao VL - 26 IS - 7 N2 - This research aimed to study the effect of distillage recycling on ethanol fermentation, the key glycolytic enzymes and cell composition of the self-flocculating yeast. With the self-flocculating yeast SPSC01 and medium composed of 220 g/L glucose, 8 g/L yeast extract and 6 g/L peptone, continuous ethanol fermentation was carried out at the dilution rate of 0.04 h(-1) with a 1.5 L tank bioreactor. Fermentation broth was collected every 3 days, and ethanol and other volatile byproducts were removed by distillation, but the stillage with high boiling byproducts was recycled to prepare the medium instead of fresh water. The system was run for 20 days, during which ethanol and biomass concentrations in the effluent decreased continuously, indicating the significant inhibition of the high boiling byproducts accumulated within the system. Thus, the activities of the key enzymes of the glycolytic pathway: hexokinase, 6-phosphofructose kinase, and pyruvate kinase were analyzed, and it was observed that all of them were inhibited. Furthermore, the biosynthesis of the stress response metabolites glycerol and trehalose was investigated, and it was found that glycerol production that can protect yeast cells against osmotic pressure stress was enhanced, but trehalose biosynthesis that can protect yeast cells against ethanol inhibition was not improved, correspondingly. And in the meantime, the biosynthesis of the major intracellular components proteins and hydrocarbons was adjusted, correspondingly. SN - 1000-3061 UR - https://www.unboundmedicine.com/medline/citation/20954406/[Impact_of_distillage_recycling_on_the_glycolysis_key_enzymes_stress_response_metabolites_and_intracelluler_components_of_the_self_flocculating_yeast]_ L2 - https://www.yeastgenome.org/reference/20954406 DB - PRIME DP - Unbound Medicine ER -