Tags

Type your tag names separated by a space and hit enter

Rapid electrochemical detection of polyaniline-labeled Escherichia coli O157:H7.
Biosens Bioelectron. 2011 Jan 15; 26(5):2208-14.BB

Abstract

There is a high demand for rapid, sensitive, and field-ready detection methods for Escherichia coli O157:H7, a highly infectious and potentially fatal food and water borne pathogen. In this study, E. coli O157:H7 cells are isolated via immunomagnetic separation (IMS) and labeled with biofunctionalized electroactive polyaniline (immuno-PANI). Labeled cell complexes are deposited onto a disposable screen-printed carbon electrode (SPCE) sensor and pulled to the electrode surface by an external magnetic field, to amplify the electrochemical signal generated by the polyaniline. Cyclic voltammetry is used to detect polyaniline and signal magnitude indicates the presence or absence of E. coli O157:H7. As few as 7CFU of E. coli O157:H7 (corresponding to an original concentration of 70 CFU/ml) were successfully detected on the SPCE sensor. The assay requires 70 min from sampling to detection, giving it a major advantage over standard culture methods in applications requiring high-throughput screening of samples and rapid results. The method can be performed with portable, handheld instrumentation and no biological modification of the sensor surface is required. Potential applications include field-based pathogen detection for food and water safety, environmental monitoring, healthcare, and biodefense.

Links

Publisher Full Text

Authors+Show Affiliations

Setterington EB
Biosystems and Agricultural Engineering, 115 Farrall Hall, Michigan State University, 48824, USA. ebs@msu.edu
Alocilja EC
No affiliation info available

MeSH

Aniline CompoundsBiosensing TechniquesColony Count, MicrobialConductometryEquipment DesignEquipment Failure AnalysisEscherichia coli O157Staining and Labeling

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

20956078

Citation

Setterington, Emma B., and Evangelyn C. Alocilja. "Rapid Electrochemical Detection of Polyaniline-labeled Escherichia Coli O157:H7." Biosensors & Bioelectronics, vol. 26, no. 5, 2011, pp. 2208-14.
Setterington EB, Alocilja EC. Rapid electrochemical detection of polyaniline-labeled Escherichia coli O157:H7. Biosens Bioelectron. 2011;26(5):2208-14.
Setterington, E. B., & Alocilja, E. C. (2011). Rapid electrochemical detection of polyaniline-labeled Escherichia coli O157:H7. Biosensors & Bioelectronics, 26(5), 2208-14. https://doi.org/10.1016/j.bios.2010.09.036
Setterington EB, Alocilja EC. Rapid Electrochemical Detection of Polyaniline-labeled Escherichia Coli O157:H7. Biosens Bioelectron. 2011 Jan 15;26(5):2208-14. PubMed PMID: 20956078.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid electrochemical detection of polyaniline-labeled Escherichia coli O157:H7. AU - Setterington,Emma B, AU - Alocilja,Evangelyn C, Y1 - 2010/09/25/ PY - 2010/08/12/received PY - 2010/09/16/revised PY - 2010/09/18/accepted PY - 2010/10/20/entrez PY - 2010/10/20/pubmed PY - 2011/4/16/medline SP - 2208 EP - 14 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 26 IS - 5 N2 - There is a high demand for rapid, sensitive, and field-ready detection methods for Escherichia coli O157:H7, a highly infectious and potentially fatal food and water borne pathogen. In this study, E. coli O157:H7 cells are isolated via immunomagnetic separation (IMS) and labeled with biofunctionalized electroactive polyaniline (immuno-PANI). Labeled cell complexes are deposited onto a disposable screen-printed carbon electrode (SPCE) sensor and pulled to the electrode surface by an external magnetic field, to amplify the electrochemical signal generated by the polyaniline. Cyclic voltammetry is used to detect polyaniline and signal magnitude indicates the presence or absence of E. coli O157:H7. As few as 7CFU of E. coli O157:H7 (corresponding to an original concentration of 70 CFU/ml) were successfully detected on the SPCE sensor. The assay requires 70 min from sampling to detection, giving it a major advantage over standard culture methods in applications requiring high-throughput screening of samples and rapid results. The method can be performed with portable, handheld instrumentation and no biological modification of the sensor surface is required. Potential applications include field-based pathogen detection for food and water safety, environmental monitoring, healthcare, and biodefense. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/20956078/Rapid_electrochemical_detection_of_polyaniline_labeled_Escherichia_coli_O157:H7_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(10)00650-0 DB - PRIME DP - Unbound Medicine ER -