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Long term bed rest with and without vibration exercise countermeasures: effects on human muscle protein dysregulation.
Proteomics. 2010 Nov; 10(21):3756-74.P

Abstract

The present investigation, the first in the field, was aimed at analyzing differentially, on individual samples, the effects of 55 days of horizontal bed rest, a model for microgravity, on myosin heavy and myosin light chain isoforms distribution (by SDS) and on the proteome (by 2-D DIGE and MS) in the vastus lateralis (VL), a mixed type II/I (∼50:50%) head of the quadriceps and in the calf soleus (SOL), a predominantly slow (∼35:65%) twitch muscle. Two separate studies were performed on six subjects without (BR) and six with resistive vibration exercise (RVE) countermeasures, respectively. Both VL and SOL underwent in BR decrements of ∼15% in cross-sectional area and of ∼22% in maximal torque that were prevented by RVE. Myosin heavy chain distribution showed increased type I and decreased type IIA in BR both in VL and in SOL, the opposite with RVE. A substantial downregulation of proteins involved in aerobic metabolism characterized both in SOL and VL in BR. RVE reversed the pattern more in VL than in SOL, whereas proteins involved in anaerobic glycolysis were upregulated. Proteins from the Z-disk region and from costamers were differently dysregulated during bed rest (both BR and RVE), particularly in VL.

Authors+Show Affiliations

Dipartimento di Scienze e Tecnologie Biomediche, Università degli Studi di Milano, Milano, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20957755

Citation

Moriggi, Manuela, et al. "Long Term Bed Rest With and Without Vibration Exercise Countermeasures: Effects On Human Muscle Protein Dysregulation." Proteomics, vol. 10, no. 21, 2010, pp. 3756-74.
Moriggi M, Vasso M, Fania C, et al. Long term bed rest with and without vibration exercise countermeasures: effects on human muscle protein dysregulation. Proteomics. 2010;10(21):3756-74.
Moriggi, M., Vasso, M., Fania, C., Capitanio, D., Bonifacio, G., Salanova, M., Blottner, D., Rittweger, J., Felsenberg, D., Cerretelli, P., & Gelfi, C. (2010). Long term bed rest with and without vibration exercise countermeasures: effects on human muscle protein dysregulation. Proteomics, 10(21), 3756-74. https://doi.org/10.1002/pmic.200900817
Moriggi M, et al. Long Term Bed Rest With and Without Vibration Exercise Countermeasures: Effects On Human Muscle Protein Dysregulation. Proteomics. 2010;10(21):3756-74. PubMed PMID: 20957755.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Long term bed rest with and without vibration exercise countermeasures: effects on human muscle protein dysregulation. AU - Moriggi,Manuela, AU - Vasso,Michele, AU - Fania,Chiara, AU - Capitanio,Daniele, AU - Bonifacio,Gaetano, AU - Salanova,Michele, AU - Blottner,Dieter, AU - Rittweger,Jörn, AU - Felsenberg,Dieter, AU - Cerretelli,Paolo, AU - Gelfi,Cecilia, PY - 2010/10/20/entrez PY - 2010/10/20/pubmed PY - 2011/3/1/medline SP - 3756 EP - 74 JF - Proteomics JO - Proteomics VL - 10 IS - 21 N2 - The present investigation, the first in the field, was aimed at analyzing differentially, on individual samples, the effects of 55 days of horizontal bed rest, a model for microgravity, on myosin heavy and myosin light chain isoforms distribution (by SDS) and on the proteome (by 2-D DIGE and MS) in the vastus lateralis (VL), a mixed type II/I (∼50:50%) head of the quadriceps and in the calf soleus (SOL), a predominantly slow (∼35:65%) twitch muscle. Two separate studies were performed on six subjects without (BR) and six with resistive vibration exercise (RVE) countermeasures, respectively. Both VL and SOL underwent in BR decrements of ∼15% in cross-sectional area and of ∼22% in maximal torque that were prevented by RVE. Myosin heavy chain distribution showed increased type I and decreased type IIA in BR both in VL and in SOL, the opposite with RVE. A substantial downregulation of proteins involved in aerobic metabolism characterized both in SOL and VL in BR. RVE reversed the pattern more in VL than in SOL, whereas proteins involved in anaerobic glycolysis were upregulated. Proteins from the Z-disk region and from costamers were differently dysregulated during bed rest (both BR and RVE), particularly in VL. SN - 1615-9861 UR - https://www.unboundmedicine.com/medline/citation/20957755/Long_term_bed_rest_with_and_without_vibration_exercise_countermeasures:_effects_on_human_muscle_protein_dysregulation_ L2 - https://doi.org/10.1002/pmic.200900817 DB - PRIME DP - Unbound Medicine ER -