Tags

Type your tag names separated by a space and hit enter

Effect of Candidatus Liberibacter asiaticus infection on susceptibility of Asian citrus psyllid, Diaphorina citri, to selected insecticides.
Pest Manag Sci. 2011 Jan; 67(1):94-9.PM

Abstract

BACKGROUND

In the present investigation, the effect of Candidatus Liberibacter asiaticus (Las), a bacterium considered to be responsible for causing huanglongbing (HLB) disease in citrus, on the physiology of its vector, the Asian citrus psyllid (ACP) Diaphorina citri Kuwayama, was determined. Specifically, the effects of Las infection on the susceptibility of ACP to selected insecticides were determined. Furthermore, total protein content and general esterase activity were quantified in Las-infected and uninfected ACP to gain insight into the possible mechanism(s) responsible for altered susceptibility to insecticides owing to Las infection.

RESULTS

LC(50) values were significantly lower in Las-infected than in uninfected ACP adults for chlorpyrifos and spinetoram. Furthermore, there was a general trend towards lower LC(50) values for three other insecticides for Las-infected ACP; however, the differences were not statistically significant. Total protein content (µg mL(-1)) was significantly lower in Las-infected (23.5 ± 1.3 in head + thorax; 27.7 ± 1.9 in abdomen) than in uninfected (29.7 ± 2.1 in head + thorax; 35.0 ± 2.3 in abdomen) ACP. Likewise, general esterase enzyme activity (nmol min(-1) mg(-1) protein) was significantly lower in Las-infected (111.6 ± 4.5 in head + thorax; 109.5 ± 3.7 in abdomen) than in uninfected (135.9 ± 7.5 in head + thorax; 206.1 ± 23.7 in abdomen) ACP.

CONCLUSION

Susceptibility of ACP to selected insecticides from five major chemistries was greater in Las-infected than in uninfected ACP. The lower total protein content and reduced general esterase activity in Las-infected than in uninfected ACP may partly explain the observed higher insecticide susceptibility of Las-infected ACP.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Tiwari S
Entomology and Nematology Department, Citrus Research and Education Center, University of Florida, Lake Alfred, FL 33850, USA.
Pelz-Stelinski K
No affiliation info available
Stelinski LL
No affiliation info available

MeSH

AnimalsEsterasesHemipteraInsect ProteinsInsecticide ResistanceInsecticidesRhizobiaceae

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20960471

Citation

Tiwari, Siddharth, et al. "Effect of Candidatus Liberibacter Asiaticus Infection On Susceptibility of Asian Citrus Psyllid, Diaphorina Citri, to Selected Insecticides." Pest Management Science, vol. 67, no. 1, 2011, pp. 94-9.
Tiwari S, Pelz-Stelinski K, Stelinski LL. Effect of Candidatus Liberibacter asiaticus infection on susceptibility of Asian citrus psyllid, Diaphorina citri, to selected insecticides. Pest Manag Sci. 2011;67(1):94-9.
Tiwari, S., Pelz-Stelinski, K., & Stelinski, L. L. (2011). Effect of Candidatus Liberibacter asiaticus infection on susceptibility of Asian citrus psyllid, Diaphorina citri, to selected insecticides. Pest Management Science, 67(1), 94-9. https://doi.org/10.1002/ps.2038
Tiwari S, Pelz-Stelinski K, Stelinski LL. Effect of Candidatus Liberibacter Asiaticus Infection On Susceptibility of Asian Citrus Psyllid, Diaphorina Citri, to Selected Insecticides. Pest Manag Sci. 2011;67(1):94-9. PubMed PMID: 20960471.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of Candidatus Liberibacter asiaticus infection on susceptibility of Asian citrus psyllid, Diaphorina citri, to selected insecticides. AU - Tiwari,Siddharth, AU - Pelz-Stelinski,Kirsten, AU - Stelinski,Lukasz L, Y1 - 2010/10/19/ PY - 2010/02/03/received PY - 2010/04/01/revised PY - 2010/07/18/accepted PY - 2010/10/21/entrez PY - 2010/10/21/pubmed PY - 2011/4/26/medline SP - 94 EP - 9 JF - Pest management science JO - Pest Manag Sci VL - 67 IS - 1 N2 - BACKGROUND: In the present investigation, the effect of Candidatus Liberibacter asiaticus (Las), a bacterium considered to be responsible for causing huanglongbing (HLB) disease in citrus, on the physiology of its vector, the Asian citrus psyllid (ACP) Diaphorina citri Kuwayama, was determined. Specifically, the effects of Las infection on the susceptibility of ACP to selected insecticides were determined. Furthermore, total protein content and general esterase activity were quantified in Las-infected and uninfected ACP to gain insight into the possible mechanism(s) responsible for altered susceptibility to insecticides owing to Las infection. RESULTS: LC(50) values were significantly lower in Las-infected than in uninfected ACP adults for chlorpyrifos and spinetoram. Furthermore, there was a general trend towards lower LC(50) values for three other insecticides for Las-infected ACP; however, the differences were not statistically significant. Total protein content (µg mL(-1)) was significantly lower in Las-infected (23.5 ± 1.3 in head + thorax; 27.7 ± 1.9 in abdomen) than in uninfected (29.7 ± 2.1 in head + thorax; 35.0 ± 2.3 in abdomen) ACP. Likewise, general esterase enzyme activity (nmol min(-1) mg(-1) protein) was significantly lower in Las-infected (111.6 ± 4.5 in head + thorax; 109.5 ± 3.7 in abdomen) than in uninfected (135.9 ± 7.5 in head + thorax; 206.1 ± 23.7 in abdomen) ACP. CONCLUSION: Susceptibility of ACP to selected insecticides from five major chemistries was greater in Las-infected than in uninfected ACP. The lower total protein content and reduced general esterase activity in Las-infected than in uninfected ACP may partly explain the observed higher insecticide susceptibility of Las-infected ACP. SN - 1526-4998 UR - https://www.unboundmedicine.com/medline/citation/20960471/Effect_of_Candidatus_Liberibacter_asiaticus_infection_on_susceptibility_of_Asian_citrus_psyllid_Diaphorina_citri_to_selected_insecticides_ L2 - https://doi.org/10.1002/ps.2038 DB - PRIME DP - Unbound Medicine ER -
Grapherence [↓11]

Related Citations

More