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Acetylcholine prevents angiotensin II-induced oxidative stress and apoptosis in H9c2 cells.
Apoptosis. 2011 Jan; 16(1):94-103.A

Abstract

Apoptosis of cardiomyocytes plays an important role in the development of cardiovascular diseases (CVD). Numerous studies have shown that generation of reactive oxygen species (ROS) induced by the renin-angiotensin system (RAS) is involved in this pathological process. Recent studies also suggested that acetylcholine (ACh) prevented the hypoxia-induced apoptosis of mouse ES cells by inhibiting the ROS production. However, whether ACh can inhibit the action of angiotensin II (Ang II) and subsequently prevent CVD development remains unclear. In this study, H9c2 cells were stimulated by 10(-6) M Ang II for 24 h with or without 10(-5) M ACh, 10(-5) M ACh + 10(-4) M atropine respectively. The results demonstrated that Ang II increased apoptosis index by fourfold (vs. the control group, P < 0.01), which were significantly diminished by ACh. However, the atropine (ACh receptor [AChR] inhibitor) treatment blocked the protective effect of ACh. Subsequently, Ang II significantly increases the expression and activity of NADPH oxidase so that ROS production is increased by sevenfold (vs. control group, P < 0.01). The activity and expression of caspase-3 along with the Bax/Bcl2 ratio and the levels of p38 mitogen activated protein kinase (MAPK) phosphorylation also appeared to follow a similar trend. Furthermore, we observed that ACh could reduce up-regulation of AT1 receptor expression induced by Ang II. However, all these effects of ACh were inhibited by atropine. In conclusion, ACh prevents Ang II-induced H9c2 cells apoptosis through down-regulation of the AT1 receptor and inhibition of ROS-mediated p38 MAPK activation as well as regulation of Bcl-2, Bax and caspase-3.

Authors+Show Affiliations

Department of Pharmacology, Xi'an Jiaotong University, College of Medicine, Xi'an, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20963497

Citation

Liu, Jin-Jun, et al. "Acetylcholine Prevents Angiotensin II-induced Oxidative Stress and Apoptosis in H9c2 Cells." Apoptosis : an International Journal On Programmed Cell Death, vol. 16, no. 1, 2011, pp. 94-103.
Liu JJ, Li DL, Zhou J, et al. Acetylcholine prevents angiotensin II-induced oxidative stress and apoptosis in H9c2 cells. Apoptosis. 2011;16(1):94-103.
Liu, J. J., Li, D. L., Zhou, J., Sun, L., Zhao, M., Kong, S. S., Wang, Y. H., Yu, X. J., Zhou, J., & Zang, W. J. (2011). Acetylcholine prevents angiotensin II-induced oxidative stress and apoptosis in H9c2 cells. Apoptosis : an International Journal On Programmed Cell Death, 16(1), 94-103. https://doi.org/10.1007/s10495-010-0549-x
Liu JJ, et al. Acetylcholine Prevents Angiotensin II-induced Oxidative Stress and Apoptosis in H9c2 Cells. Apoptosis. 2011;16(1):94-103. PubMed PMID: 20963497.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Acetylcholine prevents angiotensin II-induced oxidative stress and apoptosis in H9c2 cells. AU - Liu,Jin-Jun, AU - Li,Dong-Ling, AU - Zhou,Juan, AU - Sun,Lei, AU - Zhao,Ming, AU - Kong,Shan-Shan, AU - Wang,You-Hua, AU - Yu,Xiao-Jiang, AU - Zhou,Jun, AU - Zang,Wei-Jin, PY - 2010/10/22/entrez PY - 2010/10/22/pubmed PY - 2011/7/6/medline SP - 94 EP - 103 JF - Apoptosis : an international journal on programmed cell death JO - Apoptosis VL - 16 IS - 1 N2 - Apoptosis of cardiomyocytes plays an important role in the development of cardiovascular diseases (CVD). Numerous studies have shown that generation of reactive oxygen species (ROS) induced by the renin-angiotensin system (RAS) is involved in this pathological process. Recent studies also suggested that acetylcholine (ACh) prevented the hypoxia-induced apoptosis of mouse ES cells by inhibiting the ROS production. However, whether ACh can inhibit the action of angiotensin II (Ang II) and subsequently prevent CVD development remains unclear. In this study, H9c2 cells were stimulated by 10(-6) M Ang II for 24 h with or without 10(-5) M ACh, 10(-5) M ACh + 10(-4) M atropine respectively. The results demonstrated that Ang II increased apoptosis index by fourfold (vs. the control group, P < 0.01), which were significantly diminished by ACh. However, the atropine (ACh receptor [AChR] inhibitor) treatment blocked the protective effect of ACh. Subsequently, Ang II significantly increases the expression and activity of NADPH oxidase so that ROS production is increased by sevenfold (vs. control group, P < 0.01). The activity and expression of caspase-3 along with the Bax/Bcl2 ratio and the levels of p38 mitogen activated protein kinase (MAPK) phosphorylation also appeared to follow a similar trend. Furthermore, we observed that ACh could reduce up-regulation of AT1 receptor expression induced by Ang II. However, all these effects of ACh were inhibited by atropine. In conclusion, ACh prevents Ang II-induced H9c2 cells apoptosis through down-regulation of the AT1 receptor and inhibition of ROS-mediated p38 MAPK activation as well as regulation of Bcl-2, Bax and caspase-3. SN - 1573-675X UR - https://www.unboundmedicine.com/medline/citation/20963497/Acetylcholine_prevents_angiotensin_II_induced_oxidative_stress_and_apoptosis_in_H9c2_cells_ L2 - https://doi.org/10.1007/s10495-010-0549-x DB - PRIME DP - Unbound Medicine ER -