Tags

Type your tag names separated by a space and hit enter

Determination of 3-hydroxy pterocarpan, a novel osteogenic compound in rat plasma by liquid chromatography-tandem mass spectrometry: application to pharmacokinetics study.
Biomed Chromatogr. 2011 Jul; 25(7):843-50.BC

Abstract

A rapid, sensitive and selective LC-MS/MS method for the quantitative analysis of 3-hydroxy pterocarpan (S006-1709) in female rat plasma has been developed and validated. A Discovery RP₁₈ column was used for the chromatographic elution using acetonitrile and 0.1% acetic acid in water as mobile phase (80:20 v/v) at the flow rate of 0.5 mL/min. MS/MS analysis was performed using a triple quadrupole mass spectrometer with electrospray ionization in negative ion mode using biochanin as an internal standard (IS). Extraction of S006-1709 and IS from rat plasma was done by liquid-liquid extraction method using diethyl ether. The LC-MS/MS method was sensitive with 1.95 ng/mL as the limit of detection and 3.9 ng/mL as the lower limit of quantification. The method was linear in the concentration range of 3.9-1000 ng/mL. The percentage bias for intraday and interday accuracy was not greater than 4.2 and the %RSD for intraday and interday precision was not greater than 13.2. The recoveries of S006-1709 and IS were 73.9-79.3 and 85.7%, respectively. S006-1709 was found to be stable in various stability studies. The validated LC-MS/MS method was successfully applied for the oral pharmacokinetics study of S006-1709 at 10 mg/kg in female Sprague-Dawley rats.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Manickavasagam L
Pharmacokinetics and Metabolism Division, Central Drug Research Institute, Lucknow-226001, Uttar Pradesh, India.
Gupta S
No affiliation info available
Mishra S
No affiliation info available
Kumar A
No affiliation info available
Raghuvanshi A
No affiliation info available
Goel A
No affiliation info available
Singh D
No affiliation info available
Jain GK
No affiliation info available

MeSH

Administration, OralAnimalsChemical FractionationChromatography, LiquidDrug StabilityEstrogensFemaleIsoflavonesLinear ModelsPterocarpansRatsRats, Sprague-DawleyReproducibility of ResultsSensitivity and SpecificityTandem Mass Spectrometry

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20963772

Citation

Manickavasagam, Lakshmi, et al. "Determination of 3-hydroxy Pterocarpan, a Novel Osteogenic Compound in Rat Plasma By Liquid Chromatography-tandem Mass Spectrometry: Application to Pharmacokinetics Study." Biomedical Chromatography : BMC, vol. 25, no. 7, 2011, pp. 843-50.
Manickavasagam L, Gupta S, Mishra S, et al. Determination of 3-hydroxy pterocarpan, a novel osteogenic compound in rat plasma by liquid chromatography-tandem mass spectrometry: application to pharmacokinetics study. Biomed Chromatogr. 2011;25(7):843-50.
Manickavasagam, L., Gupta, S., Mishra, S., Kumar, A., Raghuvanshi, A., Goel, A., Singh, D., & Jain, G. K. (2011). Determination of 3-hydroxy pterocarpan, a novel osteogenic compound in rat plasma by liquid chromatography-tandem mass spectrometry: application to pharmacokinetics study. Biomedical Chromatography : BMC, 25(7), 843-50. https://doi.org/10.1002/bmc.1527
Manickavasagam L, et al. Determination of 3-hydroxy Pterocarpan, a Novel Osteogenic Compound in Rat Plasma By Liquid Chromatography-tandem Mass Spectrometry: Application to Pharmacokinetics Study. Biomed Chromatogr. 2011;25(7):843-50. PubMed PMID: 20963772.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of 3-hydroxy pterocarpan, a novel osteogenic compound in rat plasma by liquid chromatography-tandem mass spectrometry: application to pharmacokinetics study. AU - Manickavasagam,Lakshmi, AU - Gupta,Sonal, AU - Mishra,Smriti, AU - Kumar,Amit, AU - Raghuvanshi,Ashutosh, AU - Goel,Atul, AU - Singh,Divya, AU - Jain,Girish Kumar, Y1 - 2010/10/20/ PY - 2010/05/20/received PY - 2010/08/12/revised PY - 2010/08/23/accepted PY - 2010/10/22/entrez PY - 2010/10/22/pubmed PY - 2011/10/8/medline SP - 843 EP - 50 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 25 IS - 7 N2 - A rapid, sensitive and selective LC-MS/MS method for the quantitative analysis of 3-hydroxy pterocarpan (S006-1709) in female rat plasma has been developed and validated. A Discovery RP₁₈ column was used for the chromatographic elution using acetonitrile and 0.1% acetic acid in water as mobile phase (80:20 v/v) at the flow rate of 0.5 mL/min. MS/MS analysis was performed using a triple quadrupole mass spectrometer with electrospray ionization in negative ion mode using biochanin as an internal standard (IS). Extraction of S006-1709 and IS from rat plasma was done by liquid-liquid extraction method using diethyl ether. The LC-MS/MS method was sensitive with 1.95 ng/mL as the limit of detection and 3.9 ng/mL as the lower limit of quantification. The method was linear in the concentration range of 3.9-1000 ng/mL. The percentage bias for intraday and interday accuracy was not greater than 4.2 and the %RSD for intraday and interday precision was not greater than 13.2. The recoveries of S006-1709 and IS were 73.9-79.3 and 85.7%, respectively. S006-1709 was found to be stable in various stability studies. The validated LC-MS/MS method was successfully applied for the oral pharmacokinetics study of S006-1709 at 10 mg/kg in female Sprague-Dawley rats. SN - 1099-0801 UR - https://www.unboundmedicine.com/medline/citation/20963772/Determination_of_3_hydroxy_pterocarpan_a_novel_osteogenic_compound_in_rat_plasma_by_liquid_chromatography_tandem_mass_spectrometry:_application_to_pharmacokinetics_study_ L2 - https://doi.org/10.1002/bmc.1527 DB - PRIME DP - Unbound Medicine ER -