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Effects of chemically or technologically treated linseed products and docosahexaenoic acid addition to linseed oil on biohydrogenation of C18:3n-3 in vitro.
J Dairy Sci 2010; 93(11):5286-99JD

Abstract

Rumen biohydrogenation kinetics of C18:3n-3 from several chemically or technologically treated linseed products and docosahexaenoic acid (DHA; C22:6n-3) addition to linseed oil were evaluated in vitro. Linseed products evaluated were linseed oil, crushed linseed, formaldehyde treated crushed linseed, sodium hydroxide/formaldehyde treated crushed linseed, extruded whole linseed (2 processing variants), extruded crushed linseed (2 processing variants), micronized crushed linseed, commercially available extruded linseed, lipid encapsulated linseed oil, and DHA addition to linseed oil. Each product was incubated with rumen liquid using equal amounts of supplemented C18:3n-3 and fermentable substrate (freeze-dried total mixed ration) for 0, 0.5, 1, 2, 4, 6, 12, and 24h using a batch culture technique. Disappearance of C18:3n-3 was measured to estimate the fractional biohydrogenation rate and lag time according to an exponential model and to calculate effective biohydrogenation of C18:3n-3, assuming a fractional passage rate of 0.060/h. Treatments showed no differences in rumen fermentation parameters, including gas production rate and volatile fatty acid concentration. Technological pretreatment (crushing) followed by chemical treatment applied as formaldehyde of linseed resulted in effective protection of C18:3n-3 against biohydrogenation. Additional chemical pretreatment (sodium hydroxide) before applying formaldehyde treatment did not further improve the effectiveness of protection. Extrusion of whole linseed compared with extrusion of crushed linseed was effective in reducing C18:3n-3 biohydrogenation, whereas the processing variants were not different in C18:3n-3 biohydrogenation. Crushed linseed, micronized crushed linseed, lipid encapsulated linseed oil, and DHA addition to linseed oil did not reduce C18:3n-3 biohydrogenation. Compared with the other treatments, docosahexaenoic acid addition to linseed oil resulted in a comparable trans11,cis15-C18:2 biohydrogenation but a lesser trans10+11-C18:1 biohydrogenation. This suggests that addition of DHA in combination with linseed oil was effective only in inhibiting the last step of biohydrogenation from trans10+11-C18:1 to C18:0.

Authors+Show Affiliations

Animal Nutrition Group, Wageningen University, Wageningen, the Netherlands. attje-rieke.sterk@wur.nlNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Studies
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20965345

Citation

Sterk, A, et al. "Effects of Chemically or Technologically Treated Linseed Products and Docosahexaenoic Acid Addition to Linseed Oil On Biohydrogenation of C18:3n-3 in Vitro." Journal of Dairy Science, vol. 93, no. 11, 2010, pp. 5286-99.
Sterk A, Hovenier R, Vlaeminck B, et al. Effects of chemically or technologically treated linseed products and docosahexaenoic acid addition to linseed oil on biohydrogenation of C18:3n-3 in vitro. J Dairy Sci. 2010;93(11):5286-99.
Sterk, A., Hovenier, R., Vlaeminck, B., van Vuuren, A. M., Hendriks, W. H., & Dijkstra, J. (2010). Effects of chemically or technologically treated linseed products and docosahexaenoic acid addition to linseed oil on biohydrogenation of C18:3n-3 in vitro. Journal of Dairy Science, 93(11), pp. 5286-99. doi:10.3168/jds.2010-3144.
Sterk A, et al. Effects of Chemically or Technologically Treated Linseed Products and Docosahexaenoic Acid Addition to Linseed Oil On Biohydrogenation of C18:3n-3 in Vitro. J Dairy Sci. 2010;93(11):5286-99. PubMed PMID: 20965345.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of chemically or technologically treated linseed products and docosahexaenoic acid addition to linseed oil on biohydrogenation of C18:3n-3 in vitro. AU - Sterk,A, AU - Hovenier,R, AU - Vlaeminck,B, AU - van Vuuren,A M, AU - Hendriks,W H, AU - Dijkstra,J, PY - 2010/02/08/received PY - 2010/07/08/accepted PY - 2010/10/23/entrez PY - 2010/10/23/pubmed PY - 2011/1/28/medline SP - 5286 EP - 99 JF - Journal of dairy science JO - J. Dairy Sci. VL - 93 IS - 11 N2 - Rumen biohydrogenation kinetics of C18:3n-3 from several chemically or technologically treated linseed products and docosahexaenoic acid (DHA; C22:6n-3) addition to linseed oil were evaluated in vitro. Linseed products evaluated were linseed oil, crushed linseed, formaldehyde treated crushed linseed, sodium hydroxide/formaldehyde treated crushed linseed, extruded whole linseed (2 processing variants), extruded crushed linseed (2 processing variants), micronized crushed linseed, commercially available extruded linseed, lipid encapsulated linseed oil, and DHA addition to linseed oil. Each product was incubated with rumen liquid using equal amounts of supplemented C18:3n-3 and fermentable substrate (freeze-dried total mixed ration) for 0, 0.5, 1, 2, 4, 6, 12, and 24h using a batch culture technique. Disappearance of C18:3n-3 was measured to estimate the fractional biohydrogenation rate and lag time according to an exponential model and to calculate effective biohydrogenation of C18:3n-3, assuming a fractional passage rate of 0.060/h. Treatments showed no differences in rumen fermentation parameters, including gas production rate and volatile fatty acid concentration. Technological pretreatment (crushing) followed by chemical treatment applied as formaldehyde of linseed resulted in effective protection of C18:3n-3 against biohydrogenation. Additional chemical pretreatment (sodium hydroxide) before applying formaldehyde treatment did not further improve the effectiveness of protection. Extrusion of whole linseed compared with extrusion of crushed linseed was effective in reducing C18:3n-3 biohydrogenation, whereas the processing variants were not different in C18:3n-3 biohydrogenation. Crushed linseed, micronized crushed linseed, lipid encapsulated linseed oil, and DHA addition to linseed oil did not reduce C18:3n-3 biohydrogenation. Compared with the other treatments, docosahexaenoic acid addition to linseed oil resulted in a comparable trans11,cis15-C18:2 biohydrogenation but a lesser trans10+11-C18:1 biohydrogenation. This suggests that addition of DHA in combination with linseed oil was effective only in inhibiting the last step of biohydrogenation from trans10+11-C18:1 to C18:0. SN - 1525-3198 UR - https://www.unboundmedicine.com/medline/citation/20965345/Effects_of_chemically_or_technologically_treated_linseed_products_and_docosahexaenoic_acid_addition_to_linseed_oil_on_biohydrogenation_of_C18:3n_3_in_vitro_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-0302(10)00571-0 DB - PRIME DP - Unbound Medicine ER -