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Rosmarinic acid antagonized 1-methyl-4-phenylpyridinium (MPP+)-induced neurotoxicity in MES23.5 dopaminergic cells.
Int J Toxicol. 2010 Dec; 29(6):625-33.IJ

Abstract

Rosmarinic acid (RA) is a naturally occurring polyphenolic compound found in various plant families. We previously reported that RA exerted protective effects against 6-hydroxydopamine (6-OHDA)-induced neurotoxicity through antioxidative properties. In this study, we investigated whether RA could prevent effects of 1-methyl-4-phenylpyridinium (MPP(+))-induced insult in MES23.5 dopamineric cells. 1-Methyl-4-phenylpyridinium treatment decreased cell viability and dopamine content, as well as caused apoptotic morphological changes. 1-Methyl-4-phenylpyridinium-induced mitochondrial dysfunction, indicated by inhibition of activity associated with mitochondrial respiratory chain complex I, suggested mitochondrial transmembrane potential collapse and generation of reactive oxygen species. Decreased Bcl-2/Bax ratio and caspase 3 activation were also observed. Rosmarinic acid pretreatment restored the complex I activity of the mitochondrial respiratory chain and partially reversed the other damaging effects of MPP(+). Our results indicate that RA plays a neuroprotective role by ameliorating mitochondrial dysfunction against MPP(+)-induced cell apoptosis and suggest that RA has the potential to be considered an aid for prevention of Parkinson's disease.

Authors+Show Affiliations

Department of Physiology, Shandong Provincial Key Laboratory of Pathogenesis and Prevention of Neurological Disorders and State Key Disciplines: Physiology, Medical College of Qingdao University, Qingdao, 266071, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20966113

Citation

Du, Tingting, et al. "Rosmarinic Acid Antagonized 1-methyl-4-phenylpyridinium (MPP+)-induced Neurotoxicity in MES23.5 Dopaminergic Cells." International Journal of Toxicology, vol. 29, no. 6, 2010, pp. 625-33.
Du T, Li L, Song N, et al. Rosmarinic acid antagonized 1-methyl-4-phenylpyridinium (MPP+)-induced neurotoxicity in MES23.5 dopaminergic cells. Int J Toxicol. 2010;29(6):625-33.
Du, T., Li, L., Song, N., Xie, J., & Jiang, H. (2010). Rosmarinic acid antagonized 1-methyl-4-phenylpyridinium (MPP+)-induced neurotoxicity in MES23.5 dopaminergic cells. International Journal of Toxicology, 29(6), 625-33. https://doi.org/10.1177/1091581810383705
Du T, et al. Rosmarinic Acid Antagonized 1-methyl-4-phenylpyridinium (MPP+)-induced Neurotoxicity in MES23.5 Dopaminergic Cells. Int J Toxicol. 2010;29(6):625-33. PubMed PMID: 20966113.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rosmarinic acid antagonized 1-methyl-4-phenylpyridinium (MPP+)-induced neurotoxicity in MES23.5 dopaminergic cells. AU - Du,Tingting, AU - Li,Lu, AU - Song,Ning, AU - Xie,Junxia, AU - Jiang,Hong, Y1 - 2010/10/21/ PY - 2010/10/23/entrez PY - 2010/10/23/pubmed PY - 2011/7/20/medline SP - 625 EP - 33 JF - International journal of toxicology JO - Int J Toxicol VL - 29 IS - 6 N2 - Rosmarinic acid (RA) is a naturally occurring polyphenolic compound found in various plant families. We previously reported that RA exerted protective effects against 6-hydroxydopamine (6-OHDA)-induced neurotoxicity through antioxidative properties. In this study, we investigated whether RA could prevent effects of 1-methyl-4-phenylpyridinium (MPP(+))-induced insult in MES23.5 dopamineric cells. 1-Methyl-4-phenylpyridinium treatment decreased cell viability and dopamine content, as well as caused apoptotic morphological changes. 1-Methyl-4-phenylpyridinium-induced mitochondrial dysfunction, indicated by inhibition of activity associated with mitochondrial respiratory chain complex I, suggested mitochondrial transmembrane potential collapse and generation of reactive oxygen species. Decreased Bcl-2/Bax ratio and caspase 3 activation were also observed. Rosmarinic acid pretreatment restored the complex I activity of the mitochondrial respiratory chain and partially reversed the other damaging effects of MPP(+). Our results indicate that RA plays a neuroprotective role by ameliorating mitochondrial dysfunction against MPP(+)-induced cell apoptosis and suggest that RA has the potential to be considered an aid for prevention of Parkinson's disease. SN - 1092-874X UR - https://www.unboundmedicine.com/medline/citation/20966113/Rosmarinic_acid_antagonized_1_methyl_4_phenylpyridinium__MPP+__induced_neurotoxicity_in_MES23_5_dopaminergic_cells_ DB - PRIME DP - Unbound Medicine ER -