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On particle ionization/enrichment of multifunctional nanoprobes: washing/separation-free, acceleration and enrichment of microwave-assisted tryptic digestion of proteins via bare TiO2 nanoparticles in ESI-MS and comparing to MALDI-MS.
J Mass Spectrom. 2010 Dec; 45(12):1402-8.JM

Abstract

A simple, rapid, straightforward and washing/separation free of in-solution digestion method for microwave-assisted tryptic digestion of proteins (cytochrome c, lysozyme and myoglobin) using bare TiO(2) nanoparticles (NPs) prepared in aqueous solution to serve as multifunctional nanoprobes in electrospray ionization mass spectrometry (ESI-MS) was demonstrated. The current approach is termed as 'on particle ionization/enrichment (OPIE)' and it can be applied in ESI-MS, atmospheric pressure-matrix-assisted laser desorption/ionization mass spectrometry (AP-MALDI-MS) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The bare TiO(2) NPs can assist, accelerate and effectively enhance the digestion efficiency, sequence coverage and detection sensitivity of peptides for the microwave-assisted tryptic digestion of proteins in ESI-MS. The reason is attributed to the fact that proteins or partially digested proteins are easily attracted or concentrated onto the surface of TiO(2) NPs, resulting in higher efficiency of digestion reactions in the microwave experiments. Besides, the TiO(2) NPs could act as a microwave absorber to accelerate and enrich the protein fragments in a short period of time (40-60 s) from the microwave experiments in ESI-MS. Furthermore, the bare TiO(2) NPs prepared in aqueous solution exhibit high adsorption capability toward the protein fragments (peptides); thus, the OPIE approach for detecting the digested protein fragments via ESI and MALDI ionization could be achieved. The current technique is also a washing and separation-free technique for accelerating and enriching microwave-assisted tryptic digestion of proteins in the ESI-MS and MALDI-MS. It exhibits potential to be widely applied to biotechnology and proteome research in the near future.

Authors+Show Affiliations

Department of Chemistry, National Sun Yat-Sen University, 70, Lien-Hai Road, Kaohsiung 80424, Taiwan. hwu@faculty.nsysu.edu.twNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20967754

Citation

Wu, Hui-Fen, et al. "On Particle Ionization/enrichment of Multifunctional Nanoprobes: Washing/separation-free, Acceleration and Enrichment of Microwave-assisted Tryptic Digestion of Proteins Via Bare TiO2 Nanoparticles in ESI-MS and Comparing to MALDI-MS." Journal of Mass Spectrometry : JMS, vol. 45, no. 12, 2010, pp. 1402-8.
Wu HF, Agrawal K, Shrivas K, et al. On particle ionization/enrichment of multifunctional nanoprobes: washing/separation-free, acceleration and enrichment of microwave-assisted tryptic digestion of proteins via bare TiO2 nanoparticles in ESI-MS and comparing to MALDI-MS. J Mass Spectrom. 2010;45(12):1402-8.
Wu, H. F., Agrawal, K., Shrivas, K., & Lee, Y. H. (2010). On particle ionization/enrichment of multifunctional nanoprobes: washing/separation-free, acceleration and enrichment of microwave-assisted tryptic digestion of proteins via bare TiO2 nanoparticles in ESI-MS and comparing to MALDI-MS. Journal of Mass Spectrometry : JMS, 45(12), 1402-8. https://doi.org/10.1002/jms.1855
Wu HF, et al. On Particle Ionization/enrichment of Multifunctional Nanoprobes: Washing/separation-free, Acceleration and Enrichment of Microwave-assisted Tryptic Digestion of Proteins Via Bare TiO2 Nanoparticles in ESI-MS and Comparing to MALDI-MS. J Mass Spectrom. 2010;45(12):1402-8. PubMed PMID: 20967754.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - On particle ionization/enrichment of multifunctional nanoprobes: washing/separation-free, acceleration and enrichment of microwave-assisted tryptic digestion of proteins via bare TiO2 nanoparticles in ESI-MS and comparing to MALDI-MS. AU - Wu,Hui-Fen, AU - Agrawal,Kavita, AU - Shrivas,Kamlesh, AU - Lee,Yi-Hsien, PY - 2010/10/23/entrez PY - 2010/10/23/pubmed PY - 2011/4/13/medline SP - 1402 EP - 8 JF - Journal of mass spectrometry : JMS JO - J Mass Spectrom VL - 45 IS - 12 N2 - A simple, rapid, straightforward and washing/separation free of in-solution digestion method for microwave-assisted tryptic digestion of proteins (cytochrome c, lysozyme and myoglobin) using bare TiO(2) nanoparticles (NPs) prepared in aqueous solution to serve as multifunctional nanoprobes in electrospray ionization mass spectrometry (ESI-MS) was demonstrated. The current approach is termed as 'on particle ionization/enrichment (OPIE)' and it can be applied in ESI-MS, atmospheric pressure-matrix-assisted laser desorption/ionization mass spectrometry (AP-MALDI-MS) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The bare TiO(2) NPs can assist, accelerate and effectively enhance the digestion efficiency, sequence coverage and detection sensitivity of peptides for the microwave-assisted tryptic digestion of proteins in ESI-MS. The reason is attributed to the fact that proteins or partially digested proteins are easily attracted or concentrated onto the surface of TiO(2) NPs, resulting in higher efficiency of digestion reactions in the microwave experiments. Besides, the TiO(2) NPs could act as a microwave absorber to accelerate and enrich the protein fragments in a short period of time (40-60 s) from the microwave experiments in ESI-MS. Furthermore, the bare TiO(2) NPs prepared in aqueous solution exhibit high adsorption capability toward the protein fragments (peptides); thus, the OPIE approach for detecting the digested protein fragments via ESI and MALDI ionization could be achieved. The current technique is also a washing and separation-free technique for accelerating and enriching microwave-assisted tryptic digestion of proteins in the ESI-MS and MALDI-MS. It exhibits potential to be widely applied to biotechnology and proteome research in the near future. SN - 1096-9888 UR - https://www.unboundmedicine.com/medline/citation/20967754/On_particle_ionization/enrichment_of_multifunctional_nanoprobes:_washing/separation_free_acceleration_and_enrichment_of_microwave_assisted_tryptic_digestion_of_proteins_via_bare_TiO2_nanoparticles_in_ESI_MS_and_comparing_to_MALDI_MS_ L2 - https://doi.org/10.1002/jms.1855 DB - PRIME DP - Unbound Medicine ER -