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Assessments of tight junction proteins occludin, claudin 5 and scaffold proteins ZO1 and ZO2 in endothelial cells of the rat blood-brain barrier: cellular responses to neurotoxicants malathion and lead acetate.
Neurotoxicology 2011; 32(1):58-67N

Abstract

The blood-brain barrier (BBB) is essential for central nervous system (CNS) normal function. It is formed by endothelial cells with special characteristics, which confer the BBB with low permeability and high transendothelial electrical resistance (TEER). We previously demonstrated that malathion and lead, two neurotoxicants widely present in the environment, decrease TEER and increase permeability in in vitro models of the BBB. In this study we assessed tight junction disruption at the protein and gene expression levels using a rat brain microvascular endothelial cell line (RBE4) exposed to lead acetate at 10(-5)M and 10(-6)M, malathion at 10(-5)M, malaoxon at 10(-6)M, and their combinations. Cells were incubated with treatments for 2h, 4h, 8h, 16h, and 24h periods. Immunoblotting assessments demonstrated that protein levels of tight junction proteins occludin and claudin 5, and scaffold proteins ZO1 and ZO2 were decreased after treatments. Gene expression determinations did not correlate with the decreases in protein, indicating that the effects on these proteins were post-translational.

Authors+Show Affiliations

Virginia-Maryland Regional College of Veterinary Medicine, 1 Duck Pond Drive, Virginia Tech, Blacksburg, VA, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20970449

Citation

Balbuena, Pergentino, et al. "Assessments of Tight Junction Proteins Occludin, Claudin 5 and Scaffold Proteins ZO1 and ZO2 in Endothelial Cells of the Rat Blood-brain Barrier: Cellular Responses to Neurotoxicants Malathion and Lead Acetate." Neurotoxicology, vol. 32, no. 1, 2011, pp. 58-67.
Balbuena P, Li W, Ehrich M. Assessments of tight junction proteins occludin, claudin 5 and scaffold proteins ZO1 and ZO2 in endothelial cells of the rat blood-brain barrier: cellular responses to neurotoxicants malathion and lead acetate. Neurotoxicology. 2011;32(1):58-67.
Balbuena, P., Li, W., & Ehrich, M. (2011). Assessments of tight junction proteins occludin, claudin 5 and scaffold proteins ZO1 and ZO2 in endothelial cells of the rat blood-brain barrier: cellular responses to neurotoxicants malathion and lead acetate. Neurotoxicology, 32(1), pp. 58-67. doi:10.1016/j.neuro.2010.10.004.
Balbuena P, Li W, Ehrich M. Assessments of Tight Junction Proteins Occludin, Claudin 5 and Scaffold Proteins ZO1 and ZO2 in Endothelial Cells of the Rat Blood-brain Barrier: Cellular Responses to Neurotoxicants Malathion and Lead Acetate. Neurotoxicology. 2011;32(1):58-67. PubMed PMID: 20970449.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Assessments of tight junction proteins occludin, claudin 5 and scaffold proteins ZO1 and ZO2 in endothelial cells of the rat blood-brain barrier: cellular responses to neurotoxicants malathion and lead acetate. AU - Balbuena,Pergentino, AU - Li,Wen, AU - Ehrich,Marion, Y1 - 2010/10/21/ PY - 2010/07/16/received PY - 2010/10/12/revised PY - 2010/10/15/accepted PY - 2010/10/26/entrez PY - 2010/10/26/pubmed PY - 2011/12/14/medline SP - 58 EP - 67 JF - Neurotoxicology JO - Neurotoxicology VL - 32 IS - 1 N2 - The blood-brain barrier (BBB) is essential for central nervous system (CNS) normal function. It is formed by endothelial cells with special characteristics, which confer the BBB with low permeability and high transendothelial electrical resistance (TEER). We previously demonstrated that malathion and lead, two neurotoxicants widely present in the environment, decrease TEER and increase permeability in in vitro models of the BBB. In this study we assessed tight junction disruption at the protein and gene expression levels using a rat brain microvascular endothelial cell line (RBE4) exposed to lead acetate at 10(-5)M and 10(-6)M, malathion at 10(-5)M, malaoxon at 10(-6)M, and their combinations. Cells were incubated with treatments for 2h, 4h, 8h, 16h, and 24h periods. Immunoblotting assessments demonstrated that protein levels of tight junction proteins occludin and claudin 5, and scaffold proteins ZO1 and ZO2 were decreased after treatments. Gene expression determinations did not correlate with the decreases in protein, indicating that the effects on these proteins were post-translational. SN - 1872-9711 UR - https://www.unboundmedicine.com/medline/citation/20970449/Assessments_of_tight_junction_proteins_occludin_claudin_5_and_scaffold_proteins_ZO1_and_ZO2_in_endothelial_cells_of_the_rat_blood_brain_barrier:_cellular_responses_to_neurotoxicants_malathion_and_lead_acetate_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0161-813X(10)00189-0 DB - PRIME DP - Unbound Medicine ER -