TY - JOUR T1 - The use of carboxyfluorescein diacetate succinimidyl ester (CFSE) to monitor lymphocyte proliferation. AU - Quah,Benjamin J C, AU - Parish,Christopher R, Y1 - 2010/10/12/ PY - 2010/10/26/entrez PY - 2010/10/26/pubmed PY - 2010/12/14/medline JF - Journal of visualized experiments : JoVE JO - J Vis Exp IS - 44 N2 - Carboxyfluorescein succinimidyl ester (CFSE) is an effective and popular means to monitor lymphocyte division. CFSE covalently labels long-lived intracellular molecules with the fluorescent dye, carboxyfluorescein. Thus, when a CFSE-labeled cell divides, its progeny are endowed with half the number of carboxyfluorescein-tagged molecules and thus each cell division can be assessed by measuring the corresponding decrease in cell fluorescence via Flow cytometry. The capacity of CFSE to label lymphocyte populations with a high fluorescent intensity of exceptionally low variance, coupled with its low cell toxicity, make it an ideal dye to measure cell division. Since it is a fluorescein-based dye it is also compatible with a broad range of other fluorochromes making it applicable to multi-color flow cytometry. This article describes the procedures typically used for labeling mouse lymphocytes for the purpose of monitoring up to 8 cell divisions. These labeled cells can be used both for in vitro and in vivo studies. SN - 1940-087X UR - https://www.unboundmedicine.com/medline/citation/20972413/The_use_of_carboxyfluorescein_diacetate_succinimidyl_ester__CFSE__to_monitor_lymphocyte_proliferation_ L2 - https://doi.org/10.3791/2259 DB - PRIME DP - Unbound Medicine ER -