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Antiparkinsonian effects of aqueous methanolic extract of Hyoscyamus niger seeds result from its monoamine oxidase inhibitory and hydroxyl radical scavenging potency.
Neurochem Res 2011; 36(1):177-86NR

Abstract

Hyoscyamus species is one of the four plants used in Ayurveda for the treatment of Parkinson's disease (PD). Since Hyoscyamus niger was found to contain negligible levels of L-DOPA, we evaluated neuroprotective potential, if any, of characterized petroleum ether and aqueous methanol extracts of its seeds in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) model of PD in mice. Air dried authenticated H. niger seeds were sequentially extracted using petroleum ether and aqueous methanol and were characterized employing HPLC-electrochemistry and LCMS. Parkinsonian mice were treated daily twice with the extracts (125-500 mg/kg, p.o.) for two days and motor functions and striatal dopamine levels were assayed. Administration of the aqueous methanol extract (containing 0.03% w/w of L-DOPA), but not petroleum ether extract, significantly attenuated motor disabilities (akinesia, catalepsy and reduced swim score) and striatal dopamine loss in MPTP treated mice. Since the extract caused significant inhibition of monoamine oxidase activity and attenuated 1-methyl-4-phenyl pyridinium (MPP+)-induced hydroxyl radical (·OH) generation in isolated mitochondria, it is possible that the methanolic extract of Hyoscyamus niger seeds protects against parkinsonism in mice by means of its ability to inhibit increased ·OH generated in the mitochondria.

Authors+Show Affiliations

Division of Cell Biology & Physiology, Laboratory of Clinical & Experimental Neuroscience, Indian Institute of Chemical Biology (CSIR), 4 Raja S. C. Mullick Road, Jadavpur, Kolkata 700032, India.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20972705

Citation

Sengupta, T, et al. "Antiparkinsonian Effects of Aqueous Methanolic Extract of Hyoscyamus Niger Seeds Result From Its Monoamine Oxidase Inhibitory and Hydroxyl Radical Scavenging Potency." Neurochemical Research, vol. 36, no. 1, 2011, pp. 177-86.
Sengupta T, Vinayagam J, Nagashayana N, et al. Antiparkinsonian effects of aqueous methanolic extract of Hyoscyamus niger seeds result from its monoamine oxidase inhibitory and hydroxyl radical scavenging potency. Neurochem Res. 2011;36(1):177-86.
Sengupta, T., Vinayagam, J., Nagashayana, N., Gowda, B., Jaisankar, P., & Mohanakumar, K. P. (2011). Antiparkinsonian effects of aqueous methanolic extract of Hyoscyamus niger seeds result from its monoamine oxidase inhibitory and hydroxyl radical scavenging potency. Neurochemical Research, 36(1), pp. 177-86. doi:10.1007/s11064-010-0289-x.
Sengupta T, et al. Antiparkinsonian Effects of Aqueous Methanolic Extract of Hyoscyamus Niger Seeds Result From Its Monoamine Oxidase Inhibitory and Hydroxyl Radical Scavenging Potency. Neurochem Res. 2011;36(1):177-86. PubMed PMID: 20972705.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antiparkinsonian effects of aqueous methanolic extract of Hyoscyamus niger seeds result from its monoamine oxidase inhibitory and hydroxyl radical scavenging potency. AU - Sengupta,T, AU - Vinayagam,J, AU - Nagashayana,N, AU - Gowda,B, AU - Jaisankar,P, AU - Mohanakumar,K P, Y1 - 2010/10/23/ PY - 2010/09/29/accepted PY - 2010/10/26/entrez PY - 2010/10/26/pubmed PY - 2011/4/19/medline SP - 177 EP - 86 JF - Neurochemical research JO - Neurochem. Res. VL - 36 IS - 1 N2 - Hyoscyamus species is one of the four plants used in Ayurveda for the treatment of Parkinson's disease (PD). Since Hyoscyamus niger was found to contain negligible levels of L-DOPA, we evaluated neuroprotective potential, if any, of characterized petroleum ether and aqueous methanol extracts of its seeds in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) model of PD in mice. Air dried authenticated H. niger seeds were sequentially extracted using petroleum ether and aqueous methanol and were characterized employing HPLC-electrochemistry and LCMS. Parkinsonian mice were treated daily twice with the extracts (125-500 mg/kg, p.o.) for two days and motor functions and striatal dopamine levels were assayed. Administration of the aqueous methanol extract (containing 0.03% w/w of L-DOPA), but not petroleum ether extract, significantly attenuated motor disabilities (akinesia, catalepsy and reduced swim score) and striatal dopamine loss in MPTP treated mice. Since the extract caused significant inhibition of monoamine oxidase activity and attenuated 1-methyl-4-phenyl pyridinium (MPP+)-induced hydroxyl radical (·OH) generation in isolated mitochondria, it is possible that the methanolic extract of Hyoscyamus niger seeds protects against parkinsonism in mice by means of its ability to inhibit increased ·OH generated in the mitochondria. SN - 1573-6903 UR - https://www.unboundmedicine.com/medline/citation/20972705/Antiparkinsonian_effects_of_aqueous_methanolic_extract_of_Hyoscyamus_niger_seeds_result_from_its_monoamine_oxidase_inhibitory_and_hydroxyl_radical_scavenging_potency L2 - https://doi.org/10.1007/s11064-010-0289-x DB - PRIME DP - Unbound Medicine ER -