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Enteral arginase II provides ornithine for citrulline synthesis.
Am J Physiol Endocrinol Metab 2011; 300(1):E188-94AJ

Abstract

The synthesis of citrulline from arginine in the small intestine depends on the provision of ornithine. To test the hypothesis that arginase II plays a central role in the supply of ornithine for citrulline synthesis, the contribution of dietary arginine, glutamine, and proline was determined by utilizing multitracer stable isotope protocols in arginase II knockout (AII(-/-)) and wild-type (WT) mice. The lack of arginase II resulted in a lower citrulline rate of appearance (121 vs. 137 μmol·kg(-1)·h(-1)) due to a reduced availability of ornithine; ornithine supplementation was able to restore the rate of citrulline production in AII(-/-) to levels comparable with WT mice. There were significant differences in the utilization of dietary citrulline precursors. The contribution of dietary arginine to the synthesis of citrulline was reduced from 45 to 10 μmol·kg(-1)·h(-1) due to the lack of arginase II. No enteral utilization of arginine was observed in AII(-/-) mice (WT = 25 μmol·kg(-1)·h(-1)), and the contribution of dietary arginine through plasma ornithine was reduced in the transgenic mice (20 vs. 13 μmol·kg(-1)·h(-1)). Dietary glutamine and proline utilization were greater in AII(-/-) than in WT mice (20 vs. 13 and 1.4 vs. 3.7 μmol·kg(-1)·h(-1), respectively). Most of the contribution of glutamine and proline was enteral rather than through plasma ornithine. The arginase isoform present in the small intestinal mucosa has the role of providing ornithine for citrulline synthesis. The lack of arginase II results in a greater contribution of plasma ornithine and dietary glutamine and proline to the synthesis of citrulline.

Authors+Show Affiliations

US Department of Agriculture/Agricultural Research Service Children's Nutrition Research Center, Department of Pediatrics, Houston, TX, USA. marini@bcm.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

20978229

Citation

Marini, Juan C., et al. "Enteral Arginase II Provides Ornithine for Citrulline Synthesis." American Journal of Physiology. Endocrinology and Metabolism, vol. 300, no. 1, 2011, pp. E188-94.
Marini JC, Keller B, Didelija IC, et al. Enteral arginase II provides ornithine for citrulline synthesis. Am J Physiol Endocrinol Metab. 2011;300(1):E188-94.
Marini, J. C., Keller, B., Didelija, I. C., Castillo, L., & Lee, B. (2011). Enteral arginase II provides ornithine for citrulline synthesis. American Journal of Physiology. Endocrinology and Metabolism, 300(1), pp. E188-94. doi:10.1152/ajpendo.00413.2010.
Marini JC, et al. Enteral Arginase II Provides Ornithine for Citrulline Synthesis. Am J Physiol Endocrinol Metab. 2011;300(1):E188-94. PubMed PMID: 20978229.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enteral arginase II provides ornithine for citrulline synthesis. AU - Marini,Juan C, AU - Keller,Bettina, AU - Didelija,Inka Cajo, AU - Castillo,Leticia, AU - Lee,Brendan, Y1 - 2010/10/26/ PY - 2010/10/28/entrez PY - 2010/10/28/pubmed PY - 2011/2/1/medline SP - E188 EP - 94 JF - American journal of physiology. Endocrinology and metabolism JO - Am. J. Physiol. Endocrinol. Metab. VL - 300 IS - 1 N2 - The synthesis of citrulline from arginine in the small intestine depends on the provision of ornithine. To test the hypothesis that arginase II plays a central role in the supply of ornithine for citrulline synthesis, the contribution of dietary arginine, glutamine, and proline was determined by utilizing multitracer stable isotope protocols in arginase II knockout (AII(-/-)) and wild-type (WT) mice. The lack of arginase II resulted in a lower citrulline rate of appearance (121 vs. 137 μmol·kg(-1)·h(-1)) due to a reduced availability of ornithine; ornithine supplementation was able to restore the rate of citrulline production in AII(-/-) to levels comparable with WT mice. There were significant differences in the utilization of dietary citrulline precursors. The contribution of dietary arginine to the synthesis of citrulline was reduced from 45 to 10 μmol·kg(-1)·h(-1) due to the lack of arginase II. No enteral utilization of arginine was observed in AII(-/-) mice (WT = 25 μmol·kg(-1)·h(-1)), and the contribution of dietary arginine through plasma ornithine was reduced in the transgenic mice (20 vs. 13 μmol·kg(-1)·h(-1)). Dietary glutamine and proline utilization were greater in AII(-/-) than in WT mice (20 vs. 13 and 1.4 vs. 3.7 μmol·kg(-1)·h(-1), respectively). Most of the contribution of glutamine and proline was enteral rather than through plasma ornithine. The arginase isoform present in the small intestinal mucosa has the role of providing ornithine for citrulline synthesis. The lack of arginase II results in a greater contribution of plasma ornithine and dietary glutamine and proline to the synthesis of citrulline. SN - 1522-1555 UR - https://www.unboundmedicine.com/medline/citation/20978229/Enteral_arginase_II_provides_ornithine_for_citrulline_synthesis_ L2 - http://www.physiology.org/doi/full/10.1152/ajpendo.00413.2010?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -