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Molecular characterization and comparative analysis of pandemic H1N1/2009 strains with co-circulating seasonal H1N1/2009 strains from eastern India.
Arch Virol. 2011 Feb; 156(2):207-17.AV

Abstract

During the peak outbreak (July-September 2009), a total 1886 patients were screened in eastern India, of which 139 (7.37%) and 52 (2.76%) were positive for pH1N1 and seasonal H1N1, respectively. Full-length HA1, NA, NS1 and PB1-F2 genes of representative strains were sequenced. Phylogenetic analysis of deduced amino acid sequences of pH1N1 strains revealed HA1 and NS1 to be of North American swine lineage, and the NA gene of Eurasian swine lineage. Consistent with previous reports, the PB1-F2 gene of pH1N1 strains was unique due to a mutation resulting in a truncated protein of 11 aa. The HA, NA and NS1 genes of H1N1/2009 strains clustered with H1N1 strains of 2000-2009, whereas a subset of strains contained a pH1N1-like truncated PB1-F2. The truncated PB1-F2 may confer the advantage of lower pathogenicity but higher replication and infectivity to the human H1N1 strains. This is the first report of seasonal H1N1/2009 strains with a pH1N1/2009-like gene segment.

Authors+Show Affiliations

Division of Virology, National Institute of Cholera and Enteric Diseases, P-33 C.I.T. Road, Scheme XM, Beliaghata, Kolkata, West Bengal, India.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20981560

Citation

Sarkar, Mehuli, et al. "Molecular Characterization and Comparative Analysis of Pandemic H1N1/2009 Strains With Co-circulating Seasonal H1N1/2009 Strains From Eastern India." Archives of Virology, vol. 156, no. 2, 2011, pp. 207-17.
Sarkar M, Agrawal AS, Sharma Dey R, et al. Molecular characterization and comparative analysis of pandemic H1N1/2009 strains with co-circulating seasonal H1N1/2009 strains from eastern India. Arch Virol. 2011;156(2):207-17.
Sarkar, M., Agrawal, A. S., Sharma Dey, R., Chattopadhyay, S., Mullick, R., De, P., Chakrabarti, S., & Chawla-Sarkar, M. (2011). Molecular characterization and comparative analysis of pandemic H1N1/2009 strains with co-circulating seasonal H1N1/2009 strains from eastern India. Archives of Virology, 156(2), 207-17. https://doi.org/10.1007/s00705-010-0842-6
Sarkar M, et al. Molecular Characterization and Comparative Analysis of Pandemic H1N1/2009 Strains With Co-circulating Seasonal H1N1/2009 Strains From Eastern India. Arch Virol. 2011;156(2):207-17. PubMed PMID: 20981560.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular characterization and comparative analysis of pandemic H1N1/2009 strains with co-circulating seasonal H1N1/2009 strains from eastern India. AU - Sarkar,Mehuli, AU - Agrawal,Anurodh S, AU - Sharma Dey,Rakhi, AU - Chattopadhyay,Shiladitya, AU - Mullick,Ranajoy, AU - De,Papiya, AU - Chakrabarti,Sekhar, AU - Chawla-Sarkar,Mamta, Y1 - 2010/10/28/ PY - 2010/06/18/received PY - 2010/10/15/accepted PY - 2010/10/29/entrez PY - 2010/10/29/pubmed PY - 2011/3/16/medline SP - 207 EP - 17 JF - Archives of virology JO - Arch Virol VL - 156 IS - 2 N2 - During the peak outbreak (July-September 2009), a total 1886 patients were screened in eastern India, of which 139 (7.37%) and 52 (2.76%) were positive for pH1N1 and seasonal H1N1, respectively. Full-length HA1, NA, NS1 and PB1-F2 genes of representative strains were sequenced. Phylogenetic analysis of deduced amino acid sequences of pH1N1 strains revealed HA1 and NS1 to be of North American swine lineage, and the NA gene of Eurasian swine lineage. Consistent with previous reports, the PB1-F2 gene of pH1N1 strains was unique due to a mutation resulting in a truncated protein of 11 aa. The HA, NA and NS1 genes of H1N1/2009 strains clustered with H1N1 strains of 2000-2009, whereas a subset of strains contained a pH1N1-like truncated PB1-F2. The truncated PB1-F2 may confer the advantage of lower pathogenicity but higher replication and infectivity to the human H1N1 strains. This is the first report of seasonal H1N1/2009 strains with a pH1N1/2009-like gene segment. SN - 1432-8798 UR - https://www.unboundmedicine.com/medline/citation/20981560/Molecular_characterization_and_comparative_analysis_of_pandemic_H1N1/2009_strains_with_co_circulating_seasonal_H1N1/2009_strains_from_eastern_India_ L2 - https://dx.doi.org/10.1007/s00705-010-0842-6 DB - PRIME DP - Unbound Medicine ER -