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Detection of Plasmodia in acridine orange stained capillary tubes (the QBC system).
Southeast Asian J Trop Med Public Health. 1990 Dec; 21(4):534-40.SA

Abstract

The sensitivity, specificity and convenience of carrying out malaria diagnosis in acridine orange stained capillary tubes using a fluorescent microscope (the QBC system) was compared to screening for Plasmodia on conventional Giemsa stained thick smears. A dilution study revealed that the QBC is able to detect Plasmodia in as low a dilution as 5 organisms per ul. The QBC system was evaluated at a district hospital in Thailand. A preliminary study of 186 patients compared the QBC system to the routine malaria screening procedure (screening up to 30 microscopic fields on a thick smear). The sensitivity of the QBC was found to be 98.9% with a specificity of 94.4%. A second combined series of 465 febrile subjects were screened by thick smear and these results were compared to the QBC. 202 were positive for malaria on both QBC and thick smear. Sensitivity in this study was found to be 99.5% (202/203) and the specificity was 94.6% (248/262). When both series were combined, there were 14 QBC malaria positives that were not detected on thick smear, and 2 QBC malaria false negatives among the 651 patients studied. The parasite densities in these cases were between 10 and 320,000 organisms/microliters. The QBC system provided only a crude estimate of the level of parasitemia. The species of Plasmodia (P. falciparum and P. vivax) were correctly identified on QBC in 78% of cases.

Authors+Show Affiliations

Queen Saovabha Memorial Institute, Thai Red Cross Society, Bangkok.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

2098913

Citation

Pornsilapatip, J, et al. "Detection of Plasmodia in Acridine Orange Stained Capillary Tubes (the QBC System)." The Southeast Asian Journal of Tropical Medicine and Public Health, vol. 21, no. 4, 1990, pp. 534-40.
Pornsilapatip J, Namsiripongpun V, Wilde H, et al. Detection of Plasmodia in acridine orange stained capillary tubes (the QBC system). Southeast Asian J Trop Med Public Health. 1990;21(4):534-40.
Pornsilapatip, J., Namsiripongpun, V., Wilde, H., Hanvanich, M., & Chutivongse, S. (1990). Detection of Plasmodia in acridine orange stained capillary tubes (the QBC system). The Southeast Asian Journal of Tropical Medicine and Public Health, 21(4), 534-40.
Pornsilapatip J, et al. Detection of Plasmodia in Acridine Orange Stained Capillary Tubes (the QBC System). Southeast Asian J Trop Med Public Health. 1990;21(4):534-40. PubMed PMID: 2098913.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of Plasmodia in acridine orange stained capillary tubes (the QBC system). AU - Pornsilapatip,J, AU - Namsiripongpun,V, AU - Wilde,H, AU - Hanvanich,M, AU - Chutivongse,S, PY - 1990/12/1/pubmed PY - 1990/12/1/medline PY - 1990/12/1/entrez SP - 534 EP - 40 JF - The Southeast Asian journal of tropical medicine and public health JO - Southeast Asian J Trop Med Public Health VL - 21 IS - 4 N2 - The sensitivity, specificity and convenience of carrying out malaria diagnosis in acridine orange stained capillary tubes using a fluorescent microscope (the QBC system) was compared to screening for Plasmodia on conventional Giemsa stained thick smears. A dilution study revealed that the QBC is able to detect Plasmodia in as low a dilution as 5 organisms per ul. The QBC system was evaluated at a district hospital in Thailand. A preliminary study of 186 patients compared the QBC system to the routine malaria screening procedure (screening up to 30 microscopic fields on a thick smear). The sensitivity of the QBC was found to be 98.9% with a specificity of 94.4%. A second combined series of 465 febrile subjects were screened by thick smear and these results were compared to the QBC. 202 were positive for malaria on both QBC and thick smear. Sensitivity in this study was found to be 99.5% (202/203) and the specificity was 94.6% (248/262). When both series were combined, there were 14 QBC malaria positives that were not detected on thick smear, and 2 QBC malaria false negatives among the 651 patients studied. The parasite densities in these cases were between 10 and 320,000 organisms/microliters. The QBC system provided only a crude estimate of the level of parasitemia. The species of Plasmodia (P. falciparum and P. vivax) were correctly identified on QBC in 78% of cases. SN - 0125-1562 UR - https://www.unboundmedicine.com/medline/citation/2098913/Detection_of_Plasmodia_in_acridine_orange_stained_capillary_tubes__the_QBC_system__ DB - PRIME DP - Unbound Medicine ER -