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Octopine and nopaline strains of Agrobacterium tumefaciens differ in virulence; molecular characterization of the virF locus.
Plant Mol Biol. 1990 Feb; 14(2):249-59.PM

Abstract

Octopine and nopaline strains of Agrobacterium tumefaciens were found to differ in virulence on Nicotiana glauca. This difference is due to the absence of a functional virF locus, which is necessary for efficient tumorigenesis on N. glauca, from the nopaline Ti plasmids. Genetic studies and DNA sequence analysis of the virF locus revealed that virF embraces one open reading frame coding for a hydrophilic protein with a molecular mass of 22,437 Da. Transcription of virF is directed from left to right, towards the T region, and is strongly induced by the phenolic compound acetosyringone. We established that virA and virG, two genes known to be essential for induction of the vir regulon, are necessary for acetosyringone-induced virF expression, implying that virF is a member of this vir regulon. Agrobacterium virF mutants can be complemented for tumor induction by co-infection with avirulent Agrobacterium 'helper' strains. We found that such 'helper' strains must express not only the virF gene but also the vir operons virA, virB, virD and virG.

Authors+Show Affiliations

Department of Plant Molecular Biology, Leiden University, Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2101693

Citation

Melchers, L S., et al. "Octopine and Nopaline Strains of Agrobacterium Tumefaciens Differ in Virulence; Molecular Characterization of the virF Locus." Plant Molecular Biology, vol. 14, no. 2, 1990, pp. 249-59.
Melchers LS, Maroney MJ, den Dulk-Ras A, et al. Octopine and nopaline strains of Agrobacterium tumefaciens differ in virulence; molecular characterization of the virF locus. Plant Mol Biol. 1990;14(2):249-59.
Melchers, L. S., Maroney, M. J., den Dulk-Ras, A., Thompson, D. V., van Vuuren, H. A., Schilperoort, R. A., & Hooykaas, P. J. (1990). Octopine and nopaline strains of Agrobacterium tumefaciens differ in virulence; molecular characterization of the virF locus. Plant Molecular Biology, 14(2), 249-59.
Melchers LS, et al. Octopine and Nopaline Strains of Agrobacterium Tumefaciens Differ in Virulence; Molecular Characterization of the virF Locus. Plant Mol Biol. 1990;14(2):249-59. PubMed PMID: 2101693.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Octopine and nopaline strains of Agrobacterium tumefaciens differ in virulence; molecular characterization of the virF locus. AU - Melchers,L S, AU - Maroney,M J, AU - den Dulk-Ras,A, AU - Thompson,D V, AU - van Vuuren,H A, AU - Schilperoort,R A, AU - Hooykaas,P J, PY - 1990/2/1/pubmed PY - 1990/2/1/medline PY - 1990/2/1/entrez SP - 249 EP - 59 JF - Plant molecular biology JO - Plant Mol. Biol. VL - 14 IS - 2 N2 - Octopine and nopaline strains of Agrobacterium tumefaciens were found to differ in virulence on Nicotiana glauca. This difference is due to the absence of a functional virF locus, which is necessary for efficient tumorigenesis on N. glauca, from the nopaline Ti plasmids. Genetic studies and DNA sequence analysis of the virF locus revealed that virF embraces one open reading frame coding for a hydrophilic protein with a molecular mass of 22,437 Da. Transcription of virF is directed from left to right, towards the T region, and is strongly induced by the phenolic compound acetosyringone. We established that virA and virG, two genes known to be essential for induction of the vir regulon, are necessary for acetosyringone-induced virF expression, implying that virF is a member of this vir regulon. Agrobacterium virF mutants can be complemented for tumor induction by co-infection with avirulent Agrobacterium 'helper' strains. We found that such 'helper' strains must express not only the virF gene but also the vir operons virA, virB, virD and virG. SN - 0167-4412 UR - https://www.unboundmedicine.com/medline/citation/2101693/Octopine_and_nopaline_strains_of_Agrobacterium_tumefaciens_differ_in_virulence L2 - https://www.lens.org/lens/search?q=citation_id:2101693 DB - PRIME DP - Unbound Medicine ER -