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Effects of insulin-like growth factor-I and platelet-rich plasma on sciatic nerve crush injury in a rat model.
J Neurosurg 2011; 114(2):522-8JN

Abstract

OBJECT

Local administration of insulin-like growth factor-I (IGF-I) has been shown to increase the rate of axon regeneration in crush-injured and freeze-injured rat sciatic nerves. Local administration of platelet-rich plasma (PRP) has been also shown to have a measurable effect on facial nerve regeneration after transection in a rat model. The objective of the study was to compare the effects of locally administered IGF-I and PRP on the parameters of the Sciatic Function Index (SFI), sensory function (SF), axon count, and myelin thickness/axon diameter ratio (G-ratio) in a rat model of crush-injured sciatic nerves.

METHODS

The right sciatic nerve of Wistar albino rats (24 animals) was crushed using a Yasargil-Phynox aneurysm clip for 45 minutes. All animals were randomly divided into 3 groups: Group 1 (control group) was treated with saline, Group 2 was treated with IGF-I, and Group 3 was treated with PRP. Injections were performed using the tissue expander's injection port with a connecting tube directed at the crush-injured site. Functional recovery was assessed with improvement in the SFI. Recovery of sensory function was using the pinch test. Histopathological examination was performed 3 months after the injury.

RESULTS

The SFI showed an improved functional recovery in the IGF-I-treated animals (Group 2) compared with the saline-treated animals (Group 1) 30 days after the injury. In IGF-I-treated rats, sensory function returned to the baseline level significantly faster than in saline-treated and PRP-treated rats as shown in values between SF-2 and SF-7. The G-ratios were found to be significantly higher in both experimental groups than in the control group.

CONCLUSIONS

This study suggests that the application of IGF-I to the crush-injured site may expedite the functional recovery of paralyzed muscle by increasing the rate of axon regeneration.

Authors+Show Affiliations

Department of Neurosurgery, Vakif Gureba Hospital, Istanbul, Turkey.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21029038

Citation

Emel, Erhan, et al. "Effects of Insulin-like Growth factor-I and Platelet-rich Plasma On Sciatic Nerve Crush Injury in a Rat Model." Journal of Neurosurgery, vol. 114, no. 2, 2011, pp. 522-8.
Emel E, Ergün SS, Kotan D, et al. Effects of insulin-like growth factor-I and platelet-rich plasma on sciatic nerve crush injury in a rat model. J Neurosurg. 2011;114(2):522-8.
Emel, E., Ergün, S. S., Kotan, D., Gürsoy, E. B., Parman, Y., Zengin, A., & Nurten, A. (2011). Effects of insulin-like growth factor-I and platelet-rich plasma on sciatic nerve crush injury in a rat model. Journal of Neurosurgery, 114(2), pp. 522-8. doi:10.3171/2010.9.JNS091928.
Emel E, et al. Effects of Insulin-like Growth factor-I and Platelet-rich Plasma On Sciatic Nerve Crush Injury in a Rat Model. J Neurosurg. 2011;114(2):522-8. PubMed PMID: 21029038.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of insulin-like growth factor-I and platelet-rich plasma on sciatic nerve crush injury in a rat model. AU - Emel,Erhan, AU - Ergün,Selma Sönmez, AU - Kotan,Dilcan, AU - Gürsoy,Esra Başar, AU - Parman,Yeşim, AU - Zengin,Asli, AU - Nurten,Asiye, Y1 - 2010/10/29/ PY - 2010/10/30/entrez PY - 2010/10/30/pubmed PY - 2011/3/11/medline SP - 522 EP - 8 JF - Journal of neurosurgery JO - J. Neurosurg. VL - 114 IS - 2 N2 - OBJECT: Local administration of insulin-like growth factor-I (IGF-I) has been shown to increase the rate of axon regeneration in crush-injured and freeze-injured rat sciatic nerves. Local administration of platelet-rich plasma (PRP) has been also shown to have a measurable effect on facial nerve regeneration after transection in a rat model. The objective of the study was to compare the effects of locally administered IGF-I and PRP on the parameters of the Sciatic Function Index (SFI), sensory function (SF), axon count, and myelin thickness/axon diameter ratio (G-ratio) in a rat model of crush-injured sciatic nerves. METHODS: The right sciatic nerve of Wistar albino rats (24 animals) was crushed using a Yasargil-Phynox aneurysm clip for 45 minutes. All animals were randomly divided into 3 groups: Group 1 (control group) was treated with saline, Group 2 was treated with IGF-I, and Group 3 was treated with PRP. Injections were performed using the tissue expander's injection port with a connecting tube directed at the crush-injured site. Functional recovery was assessed with improvement in the SFI. Recovery of sensory function was using the pinch test. Histopathological examination was performed 3 months after the injury. RESULTS: The SFI showed an improved functional recovery in the IGF-I-treated animals (Group 2) compared with the saline-treated animals (Group 1) 30 days after the injury. In IGF-I-treated rats, sensory function returned to the baseline level significantly faster than in saline-treated and PRP-treated rats as shown in values between SF-2 and SF-7. The G-ratios were found to be significantly higher in both experimental groups than in the control group. CONCLUSIONS: This study suggests that the application of IGF-I to the crush-injured site may expedite the functional recovery of paralyzed muscle by increasing the rate of axon regeneration. SN - 1933-0693 UR - https://www.unboundmedicine.com/medline/citation/21029038/Effects_of_insulin_like_growth_factor_I_and_platelet_rich_plasma_on_sciatic_nerve_crush_injury_in_a_rat_model_ L2 - https://thejns.org/doi/10.3171/2010.9.JNS091928 DB - PRIME DP - Unbound Medicine ER -