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Label-free and sensitive electrogenerated chemiluminescence aptasensor for the determination of lysozyme.
Biosens Bioelectron. 2011 Jan 15; 26(5):2733-6.BB

Abstract

A novel label-free electrogenerated chemiluminescence (ECL) aptasensor for the determination of lysozyme is designed employing lysozyme binding aptamer (LBA) as molecular recognition element for lysozyme as a model analyte and Ru(bpy)(3)(2+) as an ECL signal compound. This ECL aptasensor was fabricated by self-assembling the thiolated LBA onto the surface of a gold electrode. Using this aptasensor, sensitive quantitative detection of lysozyme is realized on basis of the competition of lysozyme with Ru(bpy)(3)(2+) cation for the binding sites of LBA. In the presence of lysozyme, the aptamer sequence prefers to form the LBA-lysozyme complex, the less negative environment allows Ru(bpy)(3)(2+) cations to be less bound electrostatically to the LBAs on the electrode surface, in conjunction with the generation of a decreased ECL signal. The integrated ECL intensity versus the concentration of lysozyme was linear in the range from 6.4×10(-10) M to 6.4×10(-7) M. The detection limit was 1.2×10(-10) M. This work demonstrates that using the competition of target protein with an ECL signal compound Ru(bpy)(3)(2+) for binding sites of special aptamer confined on the electrode is promising approach for the design of label-free ECL aptasensors for the determination of proteins.

Authors+Show Affiliations

Institute of Analytical Science, Northwest University, Shaanxi Provincial Key Laboratory of Electroanalytical Chemistry, Xi'an, Shaanxi 710069, PR China. yanli@nwu.edu.cnNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21030242

Citation

Li, Yan, et al. "Label-free and Sensitive Electrogenerated Chemiluminescence Aptasensor for the Determination of Lysozyme." Biosensors & Bioelectronics, vol. 26, no. 5, 2011, pp. 2733-6.
Li Y, Qi H, Gao Q, et al. Label-free and sensitive electrogenerated chemiluminescence aptasensor for the determination of lysozyme. Biosens Bioelectron. 2011;26(5):2733-6.
Li, Y., Qi, H., Gao, Q., & Zhang, C. (2011). Label-free and sensitive electrogenerated chemiluminescence aptasensor for the determination of lysozyme. Biosensors & Bioelectronics, 26(5), 2733-6. https://doi.org/10.1016/j.bios.2010.09.048
Li Y, et al. Label-free and Sensitive Electrogenerated Chemiluminescence Aptasensor for the Determination of Lysozyme. Biosens Bioelectron. 2011 Jan 15;26(5):2733-6. PubMed PMID: 21030242.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Label-free and sensitive electrogenerated chemiluminescence aptasensor for the determination of lysozyme. AU - Li,Yan, AU - Qi,Honglan, AU - Gao,Qiang, AU - Zhang,Chengxiao, Y1 - 2010/10/27/ PY - 2010/07/03/received PY - 2010/09/19/revised PY - 2010/09/26/accepted PY - 2010/10/30/entrez PY - 2010/10/30/pubmed PY - 2011/4/16/medline SP - 2733 EP - 6 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 26 IS - 5 N2 - A novel label-free electrogenerated chemiluminescence (ECL) aptasensor for the determination of lysozyme is designed employing lysozyme binding aptamer (LBA) as molecular recognition element for lysozyme as a model analyte and Ru(bpy)(3)(2+) as an ECL signal compound. This ECL aptasensor was fabricated by self-assembling the thiolated LBA onto the surface of a gold electrode. Using this aptasensor, sensitive quantitative detection of lysozyme is realized on basis of the competition of lysozyme with Ru(bpy)(3)(2+) cation for the binding sites of LBA. In the presence of lysozyme, the aptamer sequence prefers to form the LBA-lysozyme complex, the less negative environment allows Ru(bpy)(3)(2+) cations to be less bound electrostatically to the LBAs on the electrode surface, in conjunction with the generation of a decreased ECL signal. The integrated ECL intensity versus the concentration of lysozyme was linear in the range from 6.4×10(-10) M to 6.4×10(-7) M. The detection limit was 1.2×10(-10) M. This work demonstrates that using the competition of target protein with an ECL signal compound Ru(bpy)(3)(2+) for binding sites of special aptamer confined on the electrode is promising approach for the design of label-free ECL aptasensors for the determination of proteins. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/21030242/Label_free_and_sensitive_electrogenerated_chemiluminescence_aptasensor_for_the_determination_of_lysozyme_ DB - PRIME DP - Unbound Medicine ER -