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Agrobacterium-mediated plant transformation by novel mini-T vectors in conjunction with a high-copy vir region helper plasmid.
Plant Mol Biol 1990; 15(2):245-56PM

Abstract

A new binary vector system for Agrobacterium-mediated plant transformation was developed. A set of four mini-T vectors comprised of T-DNA border sequences from nopaline-type Ti-plasmid pTiC58 flanking a chimaeric hygromycin-resistance gene for selection of transformants and up to eight unique restriction sites for cloning foreign DNA was constructed on a broad-host replicon containing the oriV of plasmid pSa. In two of the constructs these multiple cloning sites are flanked by a strong promoter to activate transcription of inserted DNA in planta. High-efficiency transformation was prompted by a high-copy, stable virulence helper plasmid pUCD2614, which contains a cloned virulence region of pTiC58 and tandem copies of the par locus of plasmid pTAR. Southern blot hybridization and genetic analyses of the progeny of transformed plants showed that the hygromycin resistance gene was stably inherited.

Authors+Show Affiliations

Department of Plant Pathology, University of California, Davis 95616.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2103448

Citation

Zyprian, E, and C I. Kado. "Agrobacterium-mediated Plant Transformation By Novel mini-T Vectors in Conjunction With a High-copy Vir Region Helper Plasmid." Plant Molecular Biology, vol. 15, no. 2, 1990, pp. 245-56.
Zyprian E, Kado CI. Agrobacterium-mediated plant transformation by novel mini-T vectors in conjunction with a high-copy vir region helper plasmid. Plant Mol Biol. 1990;15(2):245-56.
Zyprian, E., & Kado, C. I. (1990). Agrobacterium-mediated plant transformation by novel mini-T vectors in conjunction with a high-copy vir region helper plasmid. Plant Molecular Biology, 15(2), pp. 245-56.
Zyprian E, Kado CI. Agrobacterium-mediated Plant Transformation By Novel mini-T Vectors in Conjunction With a High-copy Vir Region Helper Plasmid. Plant Mol Biol. 1990;15(2):245-56. PubMed PMID: 2103448.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Agrobacterium-mediated plant transformation by novel mini-T vectors in conjunction with a high-copy vir region helper plasmid. AU - Zyprian,E, AU - Kado,C I, PY - 1990/8/1/pubmed PY - 1990/8/1/medline PY - 1990/8/1/entrez SP - 245 EP - 56 JF - Plant molecular biology JO - Plant Mol. Biol. VL - 15 IS - 2 N2 - A new binary vector system for Agrobacterium-mediated plant transformation was developed. A set of four mini-T vectors comprised of T-DNA border sequences from nopaline-type Ti-plasmid pTiC58 flanking a chimaeric hygromycin-resistance gene for selection of transformants and up to eight unique restriction sites for cloning foreign DNA was constructed on a broad-host replicon containing the oriV of plasmid pSa. In two of the constructs these multiple cloning sites are flanked by a strong promoter to activate transcription of inserted DNA in planta. High-efficiency transformation was prompted by a high-copy, stable virulence helper plasmid pUCD2614, which contains a cloned virulence region of pTiC58 and tandem copies of the par locus of plasmid pTAR. Southern blot hybridization and genetic analyses of the progeny of transformed plants showed that the hygromycin resistance gene was stably inherited. SN - 0167-4412 UR - https://www.unboundmedicine.com/medline/citation/2103448/Agrobacterium_mediated_plant_transformation_by_novel_mini_T_vectors_in_conjunction_with_a_high_copy_vir_region_helper_plasmid_ L2 - https://www.lens.org/lens/search?q=citation_id:2103448 DB - PRIME DP - Unbound Medicine ER -