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Is an orthorhombic lateral packing and a proper lamellar organization important for the skin barrier function?
Biochim Biophys Acta. 2011 Jun; 1808(6):1529-37.BB

Abstract

The lipid organization in the stratum corneum (SC), plays an important role in the barrier function of the skin. SC lipids form two lamellar phases with a predominantly orthorhombic packing. In previous publications a lipid model was presented, referred to as the stratum corneum substitute (SCS), that closely mimics the SC lipid organization and barrier function. Therefore, the SCS serves as a unique tool to relate lipid organization with barrier function. In the present study we examined the effect of the orthorhombic to hexagonal phase transition on the barrier function of human SC and SCS. In addition, the SCS was modified by changing the free fatty acid composition, resulting in a hexagonal packing and perturbed lamellar organization. By measuring the permeability to benzoic acid as function of temperature, Arrhenius plots were constructed from which activation energies were calculated. The results suggest that the change from orthorhombic to hexagonal packing in human SC and SCS, does not have an effect on the permeability. However, the modified SCS revealed an increased permeability to benzoic acid, which we related to its perturbed lamellar organization. Thus, a proper lamellar organization is more crucial for a competent barrier function than the presence of an orthorhombic lateral packing.

Authors+Show Affiliations

Leiden/Amsterdam Center for Drug Research, Department of Drug Delivery Technology, Gorlaeus Laboratories, University of Leiden, Leiden, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21036143

Citation

Groen, Daniël, et al. "Is an Orthorhombic Lateral Packing and a Proper Lamellar Organization Important for the Skin Barrier Function?" Biochimica Et Biophysica Acta, vol. 1808, no. 6, 2011, pp. 1529-37.
Groen D, Poole DS, Gooris GS, et al. Is an orthorhombic lateral packing and a proper lamellar organization important for the skin barrier function? Biochim Biophys Acta. 2011;1808(6):1529-37.
Groen, D., Poole, D. S., Gooris, G. S., & Bouwstra, J. A. (2011). Is an orthorhombic lateral packing and a proper lamellar organization important for the skin barrier function? Biochimica Et Biophysica Acta, 1808(6), 1529-37. https://doi.org/10.1016/j.bbamem.2010.10.015
Groen D, et al. Is an Orthorhombic Lateral Packing and a Proper Lamellar Organization Important for the Skin Barrier Function. Biochim Biophys Acta. 2011;1808(6):1529-37. PubMed PMID: 21036143.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Is an orthorhombic lateral packing and a proper lamellar organization important for the skin barrier function? AU - Groen,Daniël, AU - Poole,Dana S, AU - Gooris,Gert S, AU - Bouwstra,Joke A, Y1 - 2010/10/28/ PY - 2010/07/29/received PY - 2010/10/18/revised PY - 2010/10/22/accepted PY - 2010/11/2/entrez PY - 2010/11/3/pubmed PY - 2011/7/28/medline SP - 1529 EP - 37 JF - Biochimica et biophysica acta JO - Biochim Biophys Acta VL - 1808 IS - 6 N2 - The lipid organization in the stratum corneum (SC), plays an important role in the barrier function of the skin. SC lipids form two lamellar phases with a predominantly orthorhombic packing. In previous publications a lipid model was presented, referred to as the stratum corneum substitute (SCS), that closely mimics the SC lipid organization and barrier function. Therefore, the SCS serves as a unique tool to relate lipid organization with barrier function. In the present study we examined the effect of the orthorhombic to hexagonal phase transition on the barrier function of human SC and SCS. In addition, the SCS was modified by changing the free fatty acid composition, resulting in a hexagonal packing and perturbed lamellar organization. By measuring the permeability to benzoic acid as function of temperature, Arrhenius plots were constructed from which activation energies were calculated. The results suggest that the change from orthorhombic to hexagonal packing in human SC and SCS, does not have an effect on the permeability. However, the modified SCS revealed an increased permeability to benzoic acid, which we related to its perturbed lamellar organization. Thus, a proper lamellar organization is more crucial for a competent barrier function than the presence of an orthorhombic lateral packing. SN - 0006-3002 UR - https://www.unboundmedicine.com/medline/citation/21036143/Is_an_orthorhombic_lateral_packing_and_a_proper_lamellar_organization_important_for_the_skin_barrier_function L2 - https://linkinghub.elsevier.com/retrieve/pii/S0005-2736(10)00369-X DB - PRIME DP - Unbound Medicine ER -