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Differential adjustment in gill Na+/K+- and V-ATPase activities and transporter mRNA expression during osmoregulatory acclimation in the cinnamon shrimp Macrobrachium amazonicum (Decapoda, Palaemonidae).
J Exp Biol. 2010 Nov 15; 213(Pt 22):3894-905.JE

Abstract

We evaluate osmotic and chloride (Cl(-)) regulatory capability in the diadromous shrimp Macrobrachium amazonicum, and the accompanying alterations in hemolymph osmolality and [Cl(-)], gill Na(+)/K(+)-ATPase activity, and expression of gill Na(+)/K(+)-ATPase α-subunit and V-ATPase B subunit mRNA during salinity (S) acclimation. We also characterize V-ATPase kinetics and the organization of transport-related membrane systems in the gill epithelium. Macrobrachium amazonicum strongly hyper-regulates hemolymph osmolality and [Cl(-)] in freshwater and in salinities up to 25‰ S. During a 10-day acclimation period to 25‰ S, hemolymph became isosmotic and hypo-chloremic after 5 days, [Cl(-)] alone remaining hyporegulated thereafter. Gill Na(+)/K(+)-ATPase α-subunit mRNA expression increased 6.5 times initial values after 1 h, then decreased to 3 to 4 times initial values by 24 h and to 1.5 times initial values after 10 days at 25‰ S. This increased expression was accompanied by a sharp decrease at 5 h then recovery of initial Na(+)/K(+)-ATPase activity within 24 h, declining again after 5 days, which suggests transient Cl(-) secretion. V-ATPase B-subunit mRNA expression increased 1.5-fold within 1 h, then reduced sharply to 0.3 times initial values by 5 h, and remained unchanged for the remainder of the 10-day period. V-ATPase activity dropped sharply and was negligible after a 10-day acclimation period to 21‰ S, revealing a marked downregulation of ion uptake mechanisms. The gill epithelium consists of thick, apical pillar cell flanges, the perikarya of which are coupled to an intralamellar septum. These two cell types respectively exhibit extensive apical evaginations and deep membrane invaginations, both of which are associated with numerous mitochondria, characterizing an ion transporting epithelium. These changes in Na(+)/K(+)- and V-ATPase activities and in mRNA expression during salinity acclimation appear to underpin ion uptake and Cl(-) secretion by the palaemonid shrimp gill.

Authors+Show Affiliations

Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21037069

Citation

Faleiros, Rogério Oliveira, et al. "Differential Adjustment in Gill Na+/K+- and V-ATPase Activities and Transporter mRNA Expression During Osmoregulatory Acclimation in the Cinnamon Shrimp Macrobrachium Amazonicum (Decapoda, Palaemonidae)." The Journal of Experimental Biology, vol. 213, no. Pt 22, 2010, pp. 3894-905.
Faleiros RO, Goldman MH, Furriel RP, et al. Differential adjustment in gill Na+/K+- and V-ATPase activities and transporter mRNA expression during osmoregulatory acclimation in the cinnamon shrimp Macrobrachium amazonicum (Decapoda, Palaemonidae). J Exp Biol. 2010;213(Pt 22):3894-905.
Faleiros, R. O., Goldman, M. H., Furriel, R. P., & McNamara, J. C. (2010). Differential adjustment in gill Na+/K+- and V-ATPase activities and transporter mRNA expression during osmoregulatory acclimation in the cinnamon shrimp Macrobrachium amazonicum (Decapoda, Palaemonidae). The Journal of Experimental Biology, 213(Pt 22), 3894-905. https://doi.org/10.1242/jeb.046870
Faleiros RO, et al. Differential Adjustment in Gill Na+/K+- and V-ATPase Activities and Transporter mRNA Expression During Osmoregulatory Acclimation in the Cinnamon Shrimp Macrobrachium Amazonicum (Decapoda, Palaemonidae). J Exp Biol. 2010 Nov 15;213(Pt 22):3894-905. PubMed PMID: 21037069.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential adjustment in gill Na+/K+- and V-ATPase activities and transporter mRNA expression during osmoregulatory acclimation in the cinnamon shrimp Macrobrachium amazonicum (Decapoda, Palaemonidae). AU - Faleiros,Rogério Oliveira, AU - Goldman,Maria Helena S, AU - Furriel,Rosa P M, AU - McNamara,John Campbell, PY - 2010/11/2/entrez PY - 2010/11/3/pubmed PY - 2011/2/25/medline SP - 3894 EP - 905 JF - The Journal of experimental biology JO - J Exp Biol VL - 213 IS - Pt 22 N2 - We evaluate osmotic and chloride (Cl(-)) regulatory capability in the diadromous shrimp Macrobrachium amazonicum, and the accompanying alterations in hemolymph osmolality and [Cl(-)], gill Na(+)/K(+)-ATPase activity, and expression of gill Na(+)/K(+)-ATPase α-subunit and V-ATPase B subunit mRNA during salinity (S) acclimation. We also characterize V-ATPase kinetics and the organization of transport-related membrane systems in the gill epithelium. Macrobrachium amazonicum strongly hyper-regulates hemolymph osmolality and [Cl(-)] in freshwater and in salinities up to 25‰ S. During a 10-day acclimation period to 25‰ S, hemolymph became isosmotic and hypo-chloremic after 5 days, [Cl(-)] alone remaining hyporegulated thereafter. Gill Na(+)/K(+)-ATPase α-subunit mRNA expression increased 6.5 times initial values after 1 h, then decreased to 3 to 4 times initial values by 24 h and to 1.5 times initial values after 10 days at 25‰ S. This increased expression was accompanied by a sharp decrease at 5 h then recovery of initial Na(+)/K(+)-ATPase activity within 24 h, declining again after 5 days, which suggests transient Cl(-) secretion. V-ATPase B-subunit mRNA expression increased 1.5-fold within 1 h, then reduced sharply to 0.3 times initial values by 5 h, and remained unchanged for the remainder of the 10-day period. V-ATPase activity dropped sharply and was negligible after a 10-day acclimation period to 21‰ S, revealing a marked downregulation of ion uptake mechanisms. The gill epithelium consists of thick, apical pillar cell flanges, the perikarya of which are coupled to an intralamellar septum. These two cell types respectively exhibit extensive apical evaginations and deep membrane invaginations, both of which are associated with numerous mitochondria, characterizing an ion transporting epithelium. These changes in Na(+)/K(+)- and V-ATPase activities and in mRNA expression during salinity acclimation appear to underpin ion uptake and Cl(-) secretion by the palaemonid shrimp gill. SN - 1477-9145 UR - https://www.unboundmedicine.com/medline/citation/21037069/Differential_adjustment_in_gill_Na+/K+__and_V_ATPase_activities_and_transporter_mRNA_expression_during_osmoregulatory_acclimation_in_the_cinnamon_shrimp_Macrobrachium_amazonicum__Decapoda_Palaemonidae__ L2 - http://jeb.biologists.org/cgi/pmidlookup?view=long&pmid=21037069 DB - PRIME DP - Unbound Medicine ER -