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[Fosinopril up-regulates and ameliorates the Ang II induced down-expression of klotho gene in NRK-52E].
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2010 Aug; 26(3):348-51.ZY

Abstract

OBJECTIVE

To investigate the effect of fosinopril (Fos) on regulating klotho gene expression and elucidate the mechanism of Fos regulating the Angiotensin II (AngII) -induced down-expression of klotho gene.

METHODS

Culture cells, NRK-52E, were incubated with media either AngII or Fos or both of all. Experimental groups incubated with Fos (10(-5) mol/L) were divided according to variant points of time for 0 (control), 3, 6, 12, 24 h. Different concentration of Fos was selected to incubated with culture cells for 0 (control), 10(-9) 10(-8), 10(-7), 10(-6), 10(-5) mol/L at the optimal time point (24 h). Five groups, which were A: control; B: AngII (10(-7) mol/L); C: Fos(10(-5) mol/L); D: AngII (10(-7) mol/L) + Fos(10(-5) mol/L) and E: Cells pretreated with Fos(10(-5) mol/L)12 h incubated with AngII (10(-7) mol/L) were divided to observe the effect of Fos on expression of klotho induced by AngII. RT-PCR and immunohistochemistry (IHC) were applied to evaluate the klotho mRNA and protein expression, respectively.

RESULTS

Fos up-regulated klotho mRNA in time-dependent manner, and independent of dose-dependent manner; AngII obviously decreased the levels of kloltho mRNA and protein expression in NRK-52E as compared to the control (P < 0.05), the down-regulating effect was reversed by incubating both with AngII and Fos (P < 0.05), and Fos could inhibit the down-regulated expression of klotho gene induced by Ang II in NRK-52E.

CONCLUSION

Fosinopril up-regulates klotho mRNA in time-dependent manner, and inhibits the down-regulated expression of klotho gene induced by Ang II.

Authors+Show Affiliations

Department of Nephrology, Hainan Province Hospital, Haikou 570001, China. linsd0110@163.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

21038689

Citation

Lin, Shu-Dian, et al. "[Fosinopril Up-regulates and Ameliorates the Ang II Induced Down-expression of Klotho Gene in NRK-52E]." Zhongguo Ying Yong Sheng Li Xue Za Zhi = Zhongguo Yingyong Shenglixue Zazhi = Chinese Journal of Applied Physiology, vol. 26, no. 3, 2010, pp. 348-51.
Lin SD, Zhou QL, Zhan F, et al. [Fosinopril up-regulates and ameliorates the Ang II induced down-expression of klotho gene in NRK-52E]. Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2010;26(3):348-51.
Lin, S. D., Zhou, Q. L., Zhan, F., Chen, D. J., & Li, W. N. (2010). [Fosinopril up-regulates and ameliorates the Ang II induced down-expression of klotho gene in NRK-52E]. Zhongguo Ying Yong Sheng Li Xue Za Zhi = Zhongguo Yingyong Shenglixue Zazhi = Chinese Journal of Applied Physiology, 26(3), 348-51.
Lin SD, et al. [Fosinopril Up-regulates and Ameliorates the Ang II Induced Down-expression of Klotho Gene in NRK-52E]. Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2010;26(3):348-51. PubMed PMID: 21038689.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Fosinopril up-regulates and ameliorates the Ang II induced down-expression of klotho gene in NRK-52E]. AU - Lin,Shu-Dian, AU - Zhou,Qiao-Ling, AU - Zhan,Feng, AU - Chen,Dao-Jun, AU - Li,Wen-Ning, PY - 2010/11/3/entrez PY - 2010/11/3/pubmed PY - 2012/6/5/medline SP - 348 EP - 51 JF - Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology JO - Zhongguo Ying Yong Sheng Li Xue Za Zhi VL - 26 IS - 3 N2 - OBJECTIVE: To investigate the effect of fosinopril (Fos) on regulating klotho gene expression and elucidate the mechanism of Fos regulating the Angiotensin II (AngII) -induced down-expression of klotho gene. METHODS: Culture cells, NRK-52E, were incubated with media either AngII or Fos or both of all. Experimental groups incubated with Fos (10(-5) mol/L) were divided according to variant points of time for 0 (control), 3, 6, 12, 24 h. Different concentration of Fos was selected to incubated with culture cells for 0 (control), 10(-9) 10(-8), 10(-7), 10(-6), 10(-5) mol/L at the optimal time point (24 h). Five groups, which were A: control; B: AngII (10(-7) mol/L); C: Fos(10(-5) mol/L); D: AngII (10(-7) mol/L) + Fos(10(-5) mol/L) and E: Cells pretreated with Fos(10(-5) mol/L)12 h incubated with AngII (10(-7) mol/L) were divided to observe the effect of Fos on expression of klotho induced by AngII. RT-PCR and immunohistochemistry (IHC) were applied to evaluate the klotho mRNA and protein expression, respectively. RESULTS: Fos up-regulated klotho mRNA in time-dependent manner, and independent of dose-dependent manner; AngII obviously decreased the levels of kloltho mRNA and protein expression in NRK-52E as compared to the control (P < 0.05), the down-regulating effect was reversed by incubating both with AngII and Fos (P < 0.05), and Fos could inhibit the down-regulated expression of klotho gene induced by Ang II in NRK-52E. CONCLUSION: Fosinopril up-regulates klotho mRNA in time-dependent manner, and inhibits the down-regulated expression of klotho gene induced by Ang II. SN - 1000-6834 UR - https://www.unboundmedicine.com/medline/citation/21038689/[Fosinopril_up_regulates_and_ameliorates_the_Ang_II_induced_down_expression_of_klotho_gene_in_NRK_52E]_ L2 - https://antibodies.cancer.gov/detail/CPTC-FOS-4 DB - PRIME DP - Unbound Medicine ER -