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Whey and casein labeled with L-[1-13C]leucine and muscle protein synthesis: effect of resistance exercise and protein ingestion.
Am J Physiol Endocrinol Metab. 2011 Jan; 300(1):E231-42.AJ

Abstract

Muscle protein turnover following resistance exercise and amino acid availability are relatively well described. By contrast, the beneficial effects of different sources of intact proteins in relation to exercise need further investigation. Our objective was to compare muscle anabolic responses to a single bolus intake of whey or casein after performance of heavy resistance exercise. Young male individuals were randomly assigned to participate in two protein trials (n = 9) or one control trial (n = 8). Infusion of l-[1-(13)C]leucine was carried out, and either whey, casein (0.3 g/kg lean body mass), or a noncaloric control drink was ingested immediately after exercise. l-[1-(13)C]leucine-labeled whey and casein were used while muscle protein synthesis (MPS) was assessed. Blood and muscle tissue samples were collected to measure systemic hormone and amino acid concentrations, tracer enrichments, and myofibrillar protein synthesis. Western blots were used to investigate the Akt signaling pathway. Plasma insulin and branched-chain amino acid concentrations increased to a greater extent after ingestion of whey compared with casein. Myofibrillar protein synthesis was equally increased 1-6 h postexercise after whey and casein intake, both of which were higher compared with control (P < 0.05). Phosphorylation of Akt and p70(S6K) was increased after exercise and protein intake (P < 0.05), but no differences were observed between the types of protein except for total 4E-BP1, which was higher after whey intake than after casein intake (P < 0.05). In conclusion, whey and casein intake immediately after resistance exercise results in an overall equal MPS response despite temporal differences in insulin and amino acid concentrations and 4E-BP1.

Authors+Show Affiliations

Institute of Sports Medicine Copenhagen, Department of Orthopedic Surgery M, Bispebjerg Hospital and Center for Healthy Aging, University of Copenhagen, Copenhagen NV, Denmark. s.reitelseder@gmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21045172

Citation

Reitelseder, Søren, et al. "Whey and Casein Labeled With L-[1-13C]leucine and Muscle Protein Synthesis: Effect of Resistance Exercise and Protein Ingestion." American Journal of Physiology. Endocrinology and Metabolism, vol. 300, no. 1, 2011, pp. E231-42.
Reitelseder S, Agergaard J, Doessing S, et al. Whey and casein labeled with L-[1-13C]leucine and muscle protein synthesis: effect of resistance exercise and protein ingestion. Am J Physiol Endocrinol Metab. 2011;300(1):E231-42.
Reitelseder, S., Agergaard, J., Doessing, S., Helmark, I. C., Lund, P., Kristensen, N. B., Frystyk, J., Flyvbjerg, A., Schjerling, P., van Hall, G., Kjaer, M., & Holm, L. (2011). Whey and casein labeled with L-[1-13C]leucine and muscle protein synthesis: effect of resistance exercise and protein ingestion. American Journal of Physiology. Endocrinology and Metabolism, 300(1), E231-42. https://doi.org/10.1152/ajpendo.00513.2010
Reitelseder S, et al. Whey and Casein Labeled With L-[1-13C]leucine and Muscle Protein Synthesis: Effect of Resistance Exercise and Protein Ingestion. Am J Physiol Endocrinol Metab. 2011;300(1):E231-42. PubMed PMID: 21045172.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Whey and casein labeled with L-[1-13C]leucine and muscle protein synthesis: effect of resistance exercise and protein ingestion. AU - Reitelseder,Søren, AU - Agergaard,Jakob, AU - Doessing,Simon, AU - Helmark,Ida C, AU - Lund,Peter, AU - Kristensen,Niels B, AU - Frystyk,Jan, AU - Flyvbjerg,Allan, AU - Schjerling,Peter, AU - van Hall,Gerrit, AU - Kjaer,Michael, AU - Holm,Lars, Y1 - 2010/11/02/ PY - 2010/11/4/entrez PY - 2010/11/4/pubmed PY - 2011/2/1/medline SP - E231 EP - 42 JF - American journal of physiology. Endocrinology and metabolism JO - Am J Physiol Endocrinol Metab VL - 300 IS - 1 N2 - Muscle protein turnover following resistance exercise and amino acid availability are relatively well described. By contrast, the beneficial effects of different sources of intact proteins in relation to exercise need further investigation. Our objective was to compare muscle anabolic responses to a single bolus intake of whey or casein after performance of heavy resistance exercise. Young male individuals were randomly assigned to participate in two protein trials (n = 9) or one control trial (n = 8). Infusion of l-[1-(13)C]leucine was carried out, and either whey, casein (0.3 g/kg lean body mass), or a noncaloric control drink was ingested immediately after exercise. l-[1-(13)C]leucine-labeled whey and casein were used while muscle protein synthesis (MPS) was assessed. Blood and muscle tissue samples were collected to measure systemic hormone and amino acid concentrations, tracer enrichments, and myofibrillar protein synthesis. Western blots were used to investigate the Akt signaling pathway. Plasma insulin and branched-chain amino acid concentrations increased to a greater extent after ingestion of whey compared with casein. Myofibrillar protein synthesis was equally increased 1-6 h postexercise after whey and casein intake, both of which were higher compared with control (P < 0.05). Phosphorylation of Akt and p70(S6K) was increased after exercise and protein intake (P < 0.05), but no differences were observed between the types of protein except for total 4E-BP1, which was higher after whey intake than after casein intake (P < 0.05). In conclusion, whey and casein intake immediately after resistance exercise results in an overall equal MPS response despite temporal differences in insulin and amino acid concentrations and 4E-BP1. SN - 1522-1555 UR - https://www.unboundmedicine.com/medline/citation/21045172/Whey_and_casein_labeled_with_L_[1_13C]leucine_and_muscle_protein_synthesis:_effect_of_resistance_exercise_and_protein_ingestion_ L2 - https://journals.physiology.org/doi/10.1152/ajpendo.00513.2010?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -