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Mechanism of fluorescent cocoon sex identification for silkworms Bombyx mori.
Sci China Life Sci. 2010 Nov; 53(11):1330-9.SC

Abstract

By using silkworms, Bombyx mori, fluorescent cocoon sex identification (FCSI) as an experimental material, direct fluorescence spectrometry of the cocoon surface indicates that the fluorescent color of silkworm cocoons is made up of two peaks of yellow and blue-purple fluorescence emission. The fluorescent difference between male and female cocoons is attributed to the differential absorption of yellow fluorescent substances by the midgut tissue of 5th instar female silkworms. Thin layer chromatography (TLC) and fluorescent spectra indicate that blue-purple fluorescent substances are composed of at least five blue-purple fluorescent pigments, and yellow fluorescent substances are made up of at least three. UV spectra and AlCl₃ color reaction show that the three fluorescent yellow pigments are flavonoids or their glycosides. Silkworm FCSI is due to selective absorption or accumulation of the yellow fluorescent pigments by the posterior midgut cells of female 5th instar larvae. The cells of the FCSI silkworm midgut, especially the cylinder intestinal cells of the posterior midgut have a component which is a yellow fluorescent pigment-specific binding protein that may be vigorously expressed in the 5th instar larvae.

Authors+Show Affiliations

State Engineering Laboratory of Modern Silk, Soochow University, Suzhou 215123, China. yqzhang@public1.sz.js.cnNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21046325

Citation

Zhang, YuQing, et al. "Mechanism of Fluorescent Cocoon Sex Identification for Silkworms Bombyx Mori." Science China. Life Sciences, vol. 53, no. 11, 2010, pp. 1330-9.
Zhang Y, Yu X, Shen W, et al. Mechanism of fluorescent cocoon sex identification for silkworms Bombyx mori. Sci China Life Sci. 2010;53(11):1330-9.
Zhang, Y., Yu, X., Shen, W., Ma, Y., Zhou, L., Xu, N., & Yi, S. (2010). Mechanism of fluorescent cocoon sex identification for silkworms Bombyx mori. Science China. Life Sciences, 53(11), 1330-9. https://doi.org/10.1007/s11427-010-4084-3
Zhang Y, et al. Mechanism of Fluorescent Cocoon Sex Identification for Silkworms Bombyx Mori. Sci China Life Sci. 2010;53(11):1330-9. PubMed PMID: 21046325.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mechanism of fluorescent cocoon sex identification for silkworms Bombyx mori. AU - Zhang,YuQing, AU - Yu,XiaoHua, AU - Shen,WeiDe, AU - Ma,YongLei, AU - Zhou,LiXia, AU - Xu,NaiXi, AU - Yi,ShuQian, Y1 - 2010/11/03/ PY - 2009/06/26/received PY - 2009/09/21/accepted PY - 2010/11/4/entrez PY - 2010/11/4/pubmed PY - 2011/2/9/medline SP - 1330 EP - 9 JF - Science China. Life sciences JO - Sci China Life Sci VL - 53 IS - 11 N2 - By using silkworms, Bombyx mori, fluorescent cocoon sex identification (FCSI) as an experimental material, direct fluorescence spectrometry of the cocoon surface indicates that the fluorescent color of silkworm cocoons is made up of two peaks of yellow and blue-purple fluorescence emission. The fluorescent difference between male and female cocoons is attributed to the differential absorption of yellow fluorescent substances by the midgut tissue of 5th instar female silkworms. Thin layer chromatography (TLC) and fluorescent spectra indicate that blue-purple fluorescent substances are composed of at least five blue-purple fluorescent pigments, and yellow fluorescent substances are made up of at least three. UV spectra and AlCl₃ color reaction show that the three fluorescent yellow pigments are flavonoids or their glycosides. Silkworm FCSI is due to selective absorption or accumulation of the yellow fluorescent pigments by the posterior midgut cells of female 5th instar larvae. The cells of the FCSI silkworm midgut, especially the cylinder intestinal cells of the posterior midgut have a component which is a yellow fluorescent pigment-specific binding protein that may be vigorously expressed in the 5th instar larvae. SN - 1869-1889 UR - https://www.unboundmedicine.com/medline/citation/21046325/Mechanism_of_fluorescent_cocoon_sex_identification_for_silkworms_Bombyx_mori_ DB - PRIME DP - Unbound Medicine ER -