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Phenotypic characterization of thymic prelymphoma cells of B10 mice treated with split-dose irradiation.
J Immunol. 1990 Feb 01; 144(3):849-53.JI

Abstract

Using an intrathymic injection assay on B10 Thy-1 congenic mice, it was demonstrated that thymic prelymphoma cells first developed within the thymuses from 4 to 8 days after split-dose irradiation and were detected in more than 63% of the test donor thymuses when examined at 21 and 31 days after irradiation. Moreover, some mice (25%) at 2 mo after split-dose irradiation had already developed thymic lymphomas in their thymuses. To characterize these thymic prelymphoma cells, the thymocytes from B10 Thy-1.1 mice 1 mo after irradiation were stained with anti-CD4 and anti-CD8 mAb and were sorted into four subpopulations. These fractionated cells were injected into the recipient thymuses to examine which subpopulation contained thymic prelymphoma cells. The results indicated that thymic prelymphoma cells existed mainly in CD4- CD8- and CD4- CD8+ thymocyte subpopulations and also in CD4+ CD8+ subpopulation. T cell lymphomas derived from CD4- CD8- prelymphoma cells had mainly CD4- CD8- or CD4- CD8+ phenotypes. T cell lymphomas developed from CD4- CD8+ prelymphoma cells mainly expressed CD4- CD8+ or CD4+ CD8+ phenotype. T cell lymphomas originating from CD4+ CD8+ prelymphoma cells were mainly CD4+ CD8+ but some CD4- CD8+ or CD4+ CD8- cells were also present. These thymic prelymphoma cells were further characterized phenotypically in relation to their expression of the marker defined by the mAb against J11d marker and TL-2 (thymus-leukemia) Ag, which is not expressed on normal thymocytes of B10.Thy-1.2 or B10.Thy-1.1 strain, but appears on the thymocytes of lymphomagenic irradiated mice. The results indicated that the prelymphoma cells existed in J11d+, TL-2+ cells.

Authors+Show Affiliations

Division of Physiology and Pathology, National Institute of Radiological Sciences, Chiba, Japan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2104913

Citation

Muto, M, et al. "Phenotypic Characterization of Thymic Prelymphoma Cells of B10 Mice Treated With Split-dose Irradiation." Journal of Immunology (Baltimore, Md. : 1950), vol. 144, no. 3, 1990, pp. 849-53.
Muto M, Kubo E, Kamisaku H, et al. Phenotypic characterization of thymic prelymphoma cells of B10 mice treated with split-dose irradiation. J Immunol. 1990;144(3):849-53.
Muto, M., Kubo, E., Kamisaku, H., & Sado, T. (1990). Phenotypic characterization of thymic prelymphoma cells of B10 mice treated with split-dose irradiation. Journal of Immunology (Baltimore, Md. : 1950), 144(3), 849-53.
Muto M, et al. Phenotypic Characterization of Thymic Prelymphoma Cells of B10 Mice Treated With Split-dose Irradiation. J Immunol. 1990 Feb 1;144(3):849-53. PubMed PMID: 2104913.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phenotypic characterization of thymic prelymphoma cells of B10 mice treated with split-dose irradiation. AU - Muto,M, AU - Kubo,E, AU - Kamisaku,H, AU - Sado,T, PY - 1990/2/1/pubmed PY - 1990/2/1/medline PY - 1990/2/1/entrez SP - 849 EP - 53 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 144 IS - 3 N2 - Using an intrathymic injection assay on B10 Thy-1 congenic mice, it was demonstrated that thymic prelymphoma cells first developed within the thymuses from 4 to 8 days after split-dose irradiation and were detected in more than 63% of the test donor thymuses when examined at 21 and 31 days after irradiation. Moreover, some mice (25%) at 2 mo after split-dose irradiation had already developed thymic lymphomas in their thymuses. To characterize these thymic prelymphoma cells, the thymocytes from B10 Thy-1.1 mice 1 mo after irradiation were stained with anti-CD4 and anti-CD8 mAb and were sorted into four subpopulations. These fractionated cells were injected into the recipient thymuses to examine which subpopulation contained thymic prelymphoma cells. The results indicated that thymic prelymphoma cells existed mainly in CD4- CD8- and CD4- CD8+ thymocyte subpopulations and also in CD4+ CD8+ subpopulation. T cell lymphomas derived from CD4- CD8- prelymphoma cells had mainly CD4- CD8- or CD4- CD8+ phenotypes. T cell lymphomas developed from CD4- CD8+ prelymphoma cells mainly expressed CD4- CD8+ or CD4+ CD8+ phenotype. T cell lymphomas originating from CD4+ CD8+ prelymphoma cells were mainly CD4+ CD8+ but some CD4- CD8+ or CD4+ CD8- cells were also present. These thymic prelymphoma cells were further characterized phenotypically in relation to their expression of the marker defined by the mAb against J11d marker and TL-2 (thymus-leukemia) Ag, which is not expressed on normal thymocytes of B10.Thy-1.2 or B10.Thy-1.1 strain, but appears on the thymocytes of lymphomagenic irradiated mice. The results indicated that the prelymphoma cells existed in J11d+, TL-2+ cells. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/2104913/Phenotypic_characterization_of_thymic_prelymphoma_cells_of_B10_mice_treated_with_split_dose_irradiation_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=2104913 DB - PRIME DP - Unbound Medicine ER -