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Monoclonal antibodies against excretory/secretory antigens of Angiostrongylus cantonensis.
Hybridoma (Larchmt). 2010 Oct; 29(5):447-52.H

Abstract

In the present study, four murine monoclonal antibodies (MAbs) were generated against the excretory/secretory (ES) products of Angiostrongylus cantonensis adult worms; two represented IgG1 and two represented IgM MAbs, and they were designated 12D5, 15F8, 21B7 and 14G10, respectively. Immunoblotting revealed that all of the MAbs predominantly recognized a 98 kDa antigen in the ES products of A. cantonensis adult worms, and no cross reactions were found with the whole worm antigens of some other common parasites, namely, Schistosoma japonicum, Clonorchis sinensis, Paragonimus westermani, Ascaris lumbricoides, Trichinella spiralis, Anisakis sp., Echinococcus granulosus, Taenia solium, and Spirometra erinacei. Immunolocalization showed that all of the four MAbs reacted with the cuticle of the adult parasite, the external surface of its intestinal canal and reproductive organs, and its egg and first-stage larvae in the lungs of rats experimentally infected with A. cantonensis. The generation and characterization of four specific MAbs against A. cantonensis ES antigens provide foundation for the development of specific immunological diagnostic techniques for human infections with A. cantonensis.

Authors+Show Affiliations

Department of Parasitology, College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21050048

Citation

Chen, Mu-Xin, et al. "Monoclonal Antibodies Against Excretory/secretory Antigens of Angiostrongylus Cantonensis." Hybridoma (2005), vol. 29, no. 5, 2010, pp. 447-52.
Chen MX, Zhang RL, Chen JX, et al. Monoclonal antibodies against excretory/secretory antigens of Angiostrongylus cantonensis. Hybridoma (Larchmt). 2010;29(5):447-52.
Chen, M. X., Zhang, R. L., Chen, J. X., Chen, S. H., Li, X. H., Gao, S. T., Geng, Y. J., Huang, D. N., Ai, L., Xu, M. J., & Zhu, X. Q. (2010). Monoclonal antibodies against excretory/secretory antigens of Angiostrongylus cantonensis. Hybridoma (2005), 29(5), 447-52. https://doi.org/10.1089/hyb.2010.0008
Chen MX, et al. Monoclonal Antibodies Against Excretory/secretory Antigens of Angiostrongylus Cantonensis. Hybridoma (Larchmt). 2010;29(5):447-52. PubMed PMID: 21050048.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Monoclonal antibodies against excretory/secretory antigens of Angiostrongylus cantonensis. AU - Chen,Mu-Xin, AU - Zhang,Ren-Li, AU - Chen,Jia-Xu, AU - Chen,Shao-Hong, AU - Li,Xiao-Heng, AU - Gao,Shi-Tong, AU - Geng,Yi-Jie, AU - Huang,Da-Na, AU - Ai,Lin, AU - Xu,Min-Jun, AU - Zhu,Xing-Quan, PY - 2010/11/6/entrez PY - 2010/11/6/pubmed PY - 2011/2/25/medline SP - 447 EP - 52 JF - Hybridoma (2005) JO - Hybridoma (Larchmt) VL - 29 IS - 5 N2 - In the present study, four murine monoclonal antibodies (MAbs) were generated against the excretory/secretory (ES) products of Angiostrongylus cantonensis adult worms; two represented IgG1 and two represented IgM MAbs, and they were designated 12D5, 15F8, 21B7 and 14G10, respectively. Immunoblotting revealed that all of the MAbs predominantly recognized a 98 kDa antigen in the ES products of A. cantonensis adult worms, and no cross reactions were found with the whole worm antigens of some other common parasites, namely, Schistosoma japonicum, Clonorchis sinensis, Paragonimus westermani, Ascaris lumbricoides, Trichinella spiralis, Anisakis sp., Echinococcus granulosus, Taenia solium, and Spirometra erinacei. Immunolocalization showed that all of the four MAbs reacted with the cuticle of the adult parasite, the external surface of its intestinal canal and reproductive organs, and its egg and first-stage larvae in the lungs of rats experimentally infected with A. cantonensis. The generation and characterization of four specific MAbs against A. cantonensis ES antigens provide foundation for the development of specific immunological diagnostic techniques for human infections with A. cantonensis. SN - 1557-8348 UR - https://www.unboundmedicine.com/medline/citation/21050048/Monoclonal_antibodies_against_excretory/secretory_antigens_of_Angiostrongylus_cantonensis_ L2 - https://www.liebertpub.com/doi/10.1089/hyb.2010.0008?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -