Tags

Type your tag names separated by a space and hit enter

Analysis of iridoids, secoiridoids and xanthones in Centaurium erythraea, Frasera caroliniensis and Gentiana lutea using LC-MS and RP-HPLC.
J Pharm Biomed Anal. 2011 Feb 20; 54(3):517-25.JP

Abstract

This study presents a new and validated HPLC method for the simultaneous determination of bioactive compounds in Centaurium erythraea, Frasera caroliniensis and Gentiana lutea. The iridoid loganic acid, four secoiridoids and 29 xanthones were separated on a RP-18 column, using aqueous o-phosphoric acid (0.085%, v/v) and acetonitrile as mobile phase. Phytochemical investigation of C. erythraea herb and F. caroliniensis roots resulted into isolation of 25 xanthones and three secoiridoids the structure of which was elucidated by spectroscopic means (NMR, MS and UV). 1,3,8-Trihydroxy-5,6-dimethoxyxanthone, isolated from C. erythraea, turned out to be a novel xanthone. The stability of the analytes was tested by subjecting samples to light, moisture and different temperatures. After six months of storage, decomposition of gentiopicroside and sweroside was observed. The swertiamarin content was nearly unchanged when stored at room temperature or in the refrigerator, but high temperature conditions reduced the content to 85%. In contrast, xanthones were stable under long-term, refrigerated and accelerated conditions. The established chromatographic method has been successfully applied for the quantification of the bioactive compounds in the three plants. The presence and distribution of polyoxygenated xanthones within the three members of the Gentianaceae family and their significance as analytical markers are discussed.

Links

Publisher Full Text
Aggregator Full Text

Authors+Show Affiliations

Aberham A
Institute of Pharmacy/Pharmacognosy, Center of Molecular Biosciences Innsbruck, University of Innsbruck, Innrain 52c, 6020 Innsbruck, Austria.
Pieri V
No affiliation info available
Croom EM
No affiliation info available
Ellmerer E
No affiliation info available
Stuppner H
No affiliation info available

MeSH

CentauriumChromatography, High Pressure LiquidChromatography, LiquidGentianaGentianaceaeHumansIridoid GlucosidesIridoidsMass SpectrometryPhytotherapyPlant ExtractsPlant RootsPyronesReproducibility of ResultsXanthones

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21050691

Citation

Aberham, Anita, et al. "Analysis of Iridoids, Secoiridoids and Xanthones in Centaurium Erythraea, Frasera Caroliniensis and Gentiana Lutea Using LC-MS and RP-HPLC." Journal of Pharmaceutical and Biomedical Analysis, vol. 54, no. 3, 2011, pp. 517-25.
Aberham A, Pieri V, Croom EM, et al. Analysis of iridoids, secoiridoids and xanthones in Centaurium erythraea, Frasera caroliniensis and Gentiana lutea using LC-MS and RP-HPLC. J Pharm Biomed Anal. 2011;54(3):517-25.
Aberham, A., Pieri, V., Croom, E. M., Ellmerer, E., & Stuppner, H. (2011). Analysis of iridoids, secoiridoids and xanthones in Centaurium erythraea, Frasera caroliniensis and Gentiana lutea using LC-MS and RP-HPLC. Journal of Pharmaceutical and Biomedical Analysis, 54(3), 517-25. https://doi.org/10.1016/j.jpba.2010.09.030
Aberham A, et al. Analysis of Iridoids, Secoiridoids and Xanthones in Centaurium Erythraea, Frasera Caroliniensis and Gentiana Lutea Using LC-MS and RP-HPLC. J Pharm Biomed Anal. 2011 Feb 20;54(3):517-25. PubMed PMID: 21050691.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of iridoids, secoiridoids and xanthones in Centaurium erythraea, Frasera caroliniensis and Gentiana lutea using LC-MS and RP-HPLC. AU - Aberham,Anita, AU - Pieri,Valerio, AU - Croom,Edward M,Jr AU - Ellmerer,Ernst, AU - Stuppner,Hermann, Y1 - 2010/10/01/ PY - 2010/06/19/received PY - 2010/09/17/revised PY - 2010/09/22/accepted PY - 2010/11/6/entrez PY - 2010/11/6/pubmed PY - 2011/5/21/medline SP - 517 EP - 25 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 54 IS - 3 N2 - This study presents a new and validated HPLC method for the simultaneous determination of bioactive compounds in Centaurium erythraea, Frasera caroliniensis and Gentiana lutea. The iridoid loganic acid, four secoiridoids and 29 xanthones were separated on a RP-18 column, using aqueous o-phosphoric acid (0.085%, v/v) and acetonitrile as mobile phase. Phytochemical investigation of C. erythraea herb and F. caroliniensis roots resulted into isolation of 25 xanthones and three secoiridoids the structure of which was elucidated by spectroscopic means (NMR, MS and UV). 1,3,8-Trihydroxy-5,6-dimethoxyxanthone, isolated from C. erythraea, turned out to be a novel xanthone. The stability of the analytes was tested by subjecting samples to light, moisture and different temperatures. After six months of storage, decomposition of gentiopicroside and sweroside was observed. The swertiamarin content was nearly unchanged when stored at room temperature or in the refrigerator, but high temperature conditions reduced the content to 85%. In contrast, xanthones were stable under long-term, refrigerated and accelerated conditions. The established chromatographic method has been successfully applied for the quantification of the bioactive compounds in the three plants. The presence and distribution of polyoxygenated xanthones within the three members of the Gentianaceae family and their significance as analytical markers are discussed. SN - 1873-264X UR - https://www.unboundmedicine.com/medline/citation/21050691/Analysis_of_iridoids_secoiridoids_and_xanthones_in_Centaurium_erythraea_Frasera_caroliniensis_and_Gentiana_lutea_using_LC_MS_and_RP_HPLC_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(10)00564-9 DB - PRIME DP - Unbound Medicine ER -