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Novel electrochemical sensor based on functionalized graphene for simultaneous determination of adenine and guanine in DNA.
Colloids Surf B Biointerfaces. 2011 Feb 01; 82(2):543-9.CS

Abstract

A nano-material carboxylic acid functionalized graphene (graphene-COOH) was prepared and used to construct a novel biosensor for the simultaneous detection of adenine and guanine. The direct electrooxidation behaviors of adenine and guanine on the graphene-COOH modified glassy carbon electrode (graphene-COOH/GCE) were carefully investigated by cyclic voltammetry and differential pulse voltammetry. The results indicated that both adenine and guanine showed the increase of the oxidation peak currents with the negative shift of the oxidation peak potentials in contrast to that on the bare glassy carbon electrode. The electrochemical parameters of adenine and guanine on the graphene-COOH/GCE were calculated and a simple and reliable electroanalytical method was developed for the detection of adenine and guanine, respectively. The modified electrode exhibited good behaviors in the simultaneous detection of adenine and guanine with the peak separation as 0.334V. The detection limit for individual determination of guanine and adenine was 5.0×10(-8)M and 2.5×10(-8)M (S/N=3), respectively. Furthermore, the measurements of thermally denatured single-stranded DNA were carried out and the value of (G+C)/(A+T) of single-stranded DNA was calculated as 0.80. The biosensor exhibited some advantages, such as simplicity, rapidity, high sensitivity, good reproducibility and long-term stability.

Authors+Show Affiliations

College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China. kejinghuang@163.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21050729

Citation

Huang, Ke-Jing, et al. "Novel Electrochemical Sensor Based On Functionalized Graphene for Simultaneous Determination of Adenine and Guanine in DNA." Colloids and Surfaces. B, Biointerfaces, vol. 82, no. 2, 2011, pp. 543-9.
Huang KJ, Niu DJ, Sun JY, et al. Novel electrochemical sensor based on functionalized graphene for simultaneous determination of adenine and guanine in DNA. Colloids Surf B Biointerfaces. 2011;82(2):543-9.
Huang, K. J., Niu, D. J., Sun, J. Y., Han, C. H., Wu, Z. W., Li, Y. L., & Xiong, X. Q. (2011). Novel electrochemical sensor based on functionalized graphene for simultaneous determination of adenine and guanine in DNA. Colloids and Surfaces. B, Biointerfaces, 82(2), 543-9. https://doi.org/10.1016/j.colsurfb.2010.10.014
Huang KJ, et al. Novel Electrochemical Sensor Based On Functionalized Graphene for Simultaneous Determination of Adenine and Guanine in DNA. Colloids Surf B Biointerfaces. 2011 Feb 1;82(2):543-9. PubMed PMID: 21050729.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Novel electrochemical sensor based on functionalized graphene for simultaneous determination of adenine and guanine in DNA. AU - Huang,Ke-Jing, AU - Niu,De-Jun, AU - Sun,Jun-Yong, AU - Han,Cong-Hui, AU - Wu,Zhi-Wei, AU - Li,Yan-Li, AU - Xiong,Xiao-Qin, Y1 - 2010/10/15/ PY - 2010/06/27/received PY - 2010/09/14/revised PY - 2010/10/07/accepted PY - 2010/11/6/entrez PY - 2010/11/6/pubmed PY - 2011/4/13/medline SP - 543 EP - 9 JF - Colloids and surfaces. B, Biointerfaces JO - Colloids Surf B Biointerfaces VL - 82 IS - 2 N2 - A nano-material carboxylic acid functionalized graphene (graphene-COOH) was prepared and used to construct a novel biosensor for the simultaneous detection of adenine and guanine. The direct electrooxidation behaviors of adenine and guanine on the graphene-COOH modified glassy carbon electrode (graphene-COOH/GCE) were carefully investigated by cyclic voltammetry and differential pulse voltammetry. The results indicated that both adenine and guanine showed the increase of the oxidation peak currents with the negative shift of the oxidation peak potentials in contrast to that on the bare glassy carbon electrode. The electrochemical parameters of adenine and guanine on the graphene-COOH/GCE were calculated and a simple and reliable electroanalytical method was developed for the detection of adenine and guanine, respectively. The modified electrode exhibited good behaviors in the simultaneous detection of adenine and guanine with the peak separation as 0.334V. The detection limit for individual determination of guanine and adenine was 5.0×10(-8)M and 2.5×10(-8)M (S/N=3), respectively. Furthermore, the measurements of thermally denatured single-stranded DNA were carried out and the value of (G+C)/(A+T) of single-stranded DNA was calculated as 0.80. The biosensor exhibited some advantages, such as simplicity, rapidity, high sensitivity, good reproducibility and long-term stability. SN - 1873-4367 UR - https://www.unboundmedicine.com/medline/citation/21050729/Novel_electrochemical_sensor_based_on_functionalized_graphene_for_simultaneous_determination_of_adenine_and_guanine_in_DNA_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0927-7765(10)00579-5 DB - PRIME DP - Unbound Medicine ER -