Tags

Type your tag names separated by a space and hit enter

The soy isoflavone genistein reverses oxidative and inflammatory state, neuropathic pain, neurotrophic and vasculature deficits in diabetes mouse model.
Eur J Pharmacol 2011; 650(2-3):694-702EJ

Abstract

Treatment of diabetes complications remains a substantial challenge. The aim of this study was to explore the ability of the soy isoflavone genistein in attenuating the signs that follow diabetes onset: nociceptive hypersensitivity, oxidative and inflammatory state, nerve growth factor (NGF) decrease and vascular dysfunctions. Genistein (3 and 6 mg/kg) was administered to C57BL/6J streptozotocin diabetic mice from the 2nd till the 5th week after disease induction. The hind paw withdrawal threshold to mechanical stimulation (tactile allodynia) was evaluated by a von Frey filament. The oxidative stress was assessed measuring: reactive oxygen species by fluorimetric analysis, both the lipoperoxide content, as malondialdehyde, the antioxidant enzymatic activities spectrophotometrically and the glutathione content spectrofluorimetrically. Proinflammatory cytokines and NGF were measured in the sciatic nerve by enzyme-linked immunosorbent assay. Aortic inducible (iNOS) and endothelial nitric oxide synthase (eNOS) protein content was measured by western immunoblotting. Genistein relieved diabetic peripheral painful neuropathy, reverted the proinflammatory cytokine and reactive oxygen species overproduction, and restored the NGF content in diabetic sciatic nerve. Furthermore it restored the GSH content and the GSH and GSSG ratio, improved the antioxidant enzymes activities, decreased reactive oxygen species and lipoperoxide level in the brain and liver. Finally it restored the iNOS and eNOS content and the superoxide dismutase activity in thoracic aorta. Hyperglycaemia and weight decrease were not affected. Genistein is able to reverse a diabetes established condition of allodynia, oxidative stress and inflammation, ameliorates NGF content and the vascular dysfunction, thus suggesting its possible therapeutic use for diabetes complications.

Authors+Show Affiliations

Dipartimento di Farmacologia, Chemioterapia e Tossicologia medica, Università degli Studi di Milano, via Vanvitelli 32, 20129 Milano, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21050844

Citation

Valsecchi, Anna Elisa, et al. "The Soy Isoflavone Genistein Reverses Oxidative and Inflammatory State, Neuropathic Pain, Neurotrophic and Vasculature Deficits in Diabetes Mouse Model." European Journal of Pharmacology, vol. 650, no. 2-3, 2011, pp. 694-702.
Valsecchi AE, Franchi S, Panerai AE, et al. The soy isoflavone genistein reverses oxidative and inflammatory state, neuropathic pain, neurotrophic and vasculature deficits in diabetes mouse model. Eur J Pharmacol. 2011;650(2-3):694-702.
Valsecchi, A. E., Franchi, S., Panerai, A. E., Rossi, A., Sacerdote, P., & Colleoni, M. (2011). The soy isoflavone genistein reverses oxidative and inflammatory state, neuropathic pain, neurotrophic and vasculature deficits in diabetes mouse model. European Journal of Pharmacology, 650(2-3), pp. 694-702. doi:10.1016/j.ejphar.2010.10.060.
Valsecchi AE, et al. The Soy Isoflavone Genistein Reverses Oxidative and Inflammatory State, Neuropathic Pain, Neurotrophic and Vasculature Deficits in Diabetes Mouse Model. Eur J Pharmacol. 2011 Jan 15;650(2-3):694-702. PubMed PMID: 21050844.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The soy isoflavone genistein reverses oxidative and inflammatory state, neuropathic pain, neurotrophic and vasculature deficits in diabetes mouse model. AU - Valsecchi,Anna Elisa, AU - Franchi,Silvia, AU - Panerai,Alberto Emilio, AU - Rossi,Alice, AU - Sacerdote,Paola, AU - Colleoni,Mariapia, Y1 - 2010/11/02/ PY - 2010/09/21/received PY - 2010/10/26/accepted PY - 2010/11/6/entrez PY - 2010/11/6/pubmed PY - 2011/5/3/medline SP - 694 EP - 702 JF - European journal of pharmacology JO - Eur. J. Pharmacol. VL - 650 IS - 2-3 N2 - Treatment of diabetes complications remains a substantial challenge. The aim of this study was to explore the ability of the soy isoflavone genistein in attenuating the signs that follow diabetes onset: nociceptive hypersensitivity, oxidative and inflammatory state, nerve growth factor (NGF) decrease and vascular dysfunctions. Genistein (3 and 6 mg/kg) was administered to C57BL/6J streptozotocin diabetic mice from the 2nd till the 5th week after disease induction. The hind paw withdrawal threshold to mechanical stimulation (tactile allodynia) was evaluated by a von Frey filament. The oxidative stress was assessed measuring: reactive oxygen species by fluorimetric analysis, both the lipoperoxide content, as malondialdehyde, the antioxidant enzymatic activities spectrophotometrically and the glutathione content spectrofluorimetrically. Proinflammatory cytokines and NGF were measured in the sciatic nerve by enzyme-linked immunosorbent assay. Aortic inducible (iNOS) and endothelial nitric oxide synthase (eNOS) protein content was measured by western immunoblotting. Genistein relieved diabetic peripheral painful neuropathy, reverted the proinflammatory cytokine and reactive oxygen species overproduction, and restored the NGF content in diabetic sciatic nerve. Furthermore it restored the GSH content and the GSH and GSSG ratio, improved the antioxidant enzymes activities, decreased reactive oxygen species and lipoperoxide level in the brain and liver. Finally it restored the iNOS and eNOS content and the superoxide dismutase activity in thoracic aorta. Hyperglycaemia and weight decrease were not affected. Genistein is able to reverse a diabetes established condition of allodynia, oxidative stress and inflammation, ameliorates NGF content and the vascular dysfunction, thus suggesting its possible therapeutic use for diabetes complications. SN - 1879-0712 UR - https://www.unboundmedicine.com/medline/citation/21050844/The_soy_isoflavone_genistein_reverses_oxidative_and_inflammatory_state_neuropathic_pain_neurotrophic_and_vasculature_deficits_in_diabetes_mouse_model_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(10)01087-3 DB - PRIME DP - Unbound Medicine ER -