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Diets rich in n-9, n-6 and n-3 fatty acids differentially affect the generation of inositol phosphates and of thromboxane by stimulated platelets, in the rabbit.
Biochem Pharmacol. 1990 Jan 01; 39(1):129-33.BP

Abstract

We have studied the effects of semi-synthetic diets rich in either n-9 (olive oil, OO) or n-6 (corn oil, CO), or n-3 (fish oil, FO, as MaxEPA) fatty acids on the levels of major PUFA in platelet lipids, on the generation of inositol phosphates by [3H]inositol labelled platelets after stimulation with thrombin and of thromboxane B2 (TxB2) by platelet rich plasma (PRP) after stimulation with collagen. The predicted elevations of oleic (OA), linoleic (LA) and eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids were observed in platelet lipids of each animal group, but in the MaxEPA fed group accumulation of EPA was associated with depletion of linoleic acid (LA) rather than of arachidonic acid (AA). Basal levels of inositol-tris-phosphate (IP3) in platelets were lowest in the OO group and highest in the CO group, whereas the increment after thrombin stimulation (1 unit/ml NIH) was maximal in the OO group and minimal in the FO group. Instead, when generation of TxB2 by stimulated platelets was evaluated, no appreciable difference among the various groups could be detected, in accordance with the limited modifications of platelet AA content induced by the diets. The overall data indicate that dietary fatty acids modulate the pathway of inositol phosphate generation in rabbit platelets, independently of modifications of TxB2 production.

Authors+Show Affiliations

Institute of Pharmacological Sciences, University of Milan, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

2105092

Citation

Medini, L, et al. "Diets Rich in N-9, N-6 and N-3 Fatty Acids Differentially Affect the Generation of Inositol Phosphates and of Thromboxane By Stimulated Platelets, in the Rabbit." Biochemical Pharmacology, vol. 39, no. 1, 1990, pp. 129-33.
Medini L, Colli S, Mosconi C, et al. Diets rich in n-9, n-6 and n-3 fatty acids differentially affect the generation of inositol phosphates and of thromboxane by stimulated platelets, in the rabbit. Biochem Pharmacol. 1990;39(1):129-33.
Medini, L., Colli, S., Mosconi, C., Tremoli, E., & Galli, C. (1990). Diets rich in n-9, n-6 and n-3 fatty acids differentially affect the generation of inositol phosphates and of thromboxane by stimulated platelets, in the rabbit. Biochemical Pharmacology, 39(1), 129-33.
Medini L, et al. Diets Rich in N-9, N-6 and N-3 Fatty Acids Differentially Affect the Generation of Inositol Phosphates and of Thromboxane By Stimulated Platelets, in the Rabbit. Biochem Pharmacol. 1990 Jan 1;39(1):129-33. PubMed PMID: 2105092.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diets rich in n-9, n-6 and n-3 fatty acids differentially affect the generation of inositol phosphates and of thromboxane by stimulated platelets, in the rabbit. AU - Medini,L, AU - Colli,S, AU - Mosconi,C, AU - Tremoli,E, AU - Galli,C, PY - 1990/1/1/pubmed PY - 1990/1/1/medline PY - 1990/1/1/entrez SP - 129 EP - 33 JF - Biochemical pharmacology JO - Biochem Pharmacol VL - 39 IS - 1 N2 - We have studied the effects of semi-synthetic diets rich in either n-9 (olive oil, OO) or n-6 (corn oil, CO), or n-3 (fish oil, FO, as MaxEPA) fatty acids on the levels of major PUFA in platelet lipids, on the generation of inositol phosphates by [3H]inositol labelled platelets after stimulation with thrombin and of thromboxane B2 (TxB2) by platelet rich plasma (PRP) after stimulation with collagen. The predicted elevations of oleic (OA), linoleic (LA) and eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids were observed in platelet lipids of each animal group, but in the MaxEPA fed group accumulation of EPA was associated with depletion of linoleic acid (LA) rather than of arachidonic acid (AA). Basal levels of inositol-tris-phosphate (IP3) in platelets were lowest in the OO group and highest in the CO group, whereas the increment after thrombin stimulation (1 unit/ml NIH) was maximal in the OO group and minimal in the FO group. Instead, when generation of TxB2 by stimulated platelets was evaluated, no appreciable difference among the various groups could be detected, in accordance with the limited modifications of platelet AA content induced by the diets. The overall data indicate that dietary fatty acids modulate the pathway of inositol phosphate generation in rabbit platelets, independently of modifications of TxB2 production. SN - 0006-2952 UR - https://www.unboundmedicine.com/medline/citation/2105092/Diets_rich_in_n_9_n_6_and_n_3_fatty_acids_differentially_affect_the_generation_of_inositol_phosphates_and_of_thromboxane_by_stimulated_platelets_in_the_rabbit_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0006-2952(90)90656-6 DB - PRIME DP - Unbound Medicine ER -