TY - JOUR T1 - Collaborative non-self recognition system in S-RNase-based self-incompatibility. AU - Kubo,Ken-ichi, AU - Entani,Tetsuyuki, AU - Takara,Akie, AU - Wang,Ning, AU - Fields,Allison M, AU - Hua,Zhihua, AU - Toyoda,Mamiko, AU - Kawashima,Shin-ichi, AU - Ando,Toshio, AU - Isogai,Akira, AU - Kao,Teh-hui, AU - Takayama,Seiji, PY - 2010/11/6/entrez PY - 2010/11/6/pubmed PY - 2010/12/14/medline SP - 796 EP - 9 JF - Science (New York, N.Y.) JO - Science VL - 330 IS - 6005 N2 - Self-incompatibility in flowering plants prevents inbreeding and promotes outcrossing to generate genetic diversity. In Solanaceae, a multiallelic gene, S-locus F-box (SLF), was previously shown to encode the pollen determinant in self-incompatibility. It was postulated that an SLF allelic product specifically detoxifies its non-self S-ribonucleases (S-RNases), allelic products of the pistil determinant, inside pollen tubes via the ubiquitin-26S-proteasome system, thereby allowing compatible pollinations. However, it remained puzzling how SLF, with much lower allelic sequence diversity than S-RNase, might have the capacity to recognize a large repertoire of non-self S-RNases. We used in vivo functional assays and protein interaction assays to show that in Petunia, at least three types of divergent SLF proteins function as the pollen determinant, each recognizing a subset of non-self S-RNases. Our findings reveal a collaborative non-self recognition system in plants. SN - 1095-9203 UR - https://www.unboundmedicine.com/medline/citation/21051632/Collaborative_non_self_recognition_system_in_S_RNase_based_self_incompatibility_ L2 - https://www.sciencemag.org/cgi/pmidlookup?view=long&pmid=21051632 DB - PRIME DP - Unbound Medicine ER -